Question 1

All the following are true in regard to the polymerase chain reaction (PCR) except

Accepted Answer

A) it amplifies low levels of specific DNA sequences. 
B) the target sequence to be amplified can be unknown. 
C) two short DNA primers are used. 
D) oligonucleotides act as a template. 
A) it amplifies low levels of specific DNA sequences. 
B) the target sequence to be amplified can be unknown. 
C) two short DNA primers are used. 
D) oligonucleotides act as a template.

Question 2

Match the technique with an appropriate description (use each answer only once).
-Nucleic acid sequence amplification

Accepted Answer

A) This amplifies target nucleic acid without the use of a thermocycler. A double-stranded DNA fragment is created and becomes the target for exponential amplification. 
B) An isothermal assay that targets either DNA or RNA but generates RNA as its amplified product. 
C) Oligonucleotide pairs hybridize to target sequences within the gene or the cryptic plasmid. 
D) Only RNA is targeted for amplification. 
A) This amplifies target nucleic acid without the use of a thermocycler. A double-stranded DNA fragment is created and becomes the target for exponential amplification. 
B) An isothermal assay that targets either DNA or RNA but generates RNA as its amplified product. 
C) Oligonucleotide pairs hybridize to target sequences within the gene or the cryptic plasmid. 
D) Only RNA is targeted for amplification.

Question 3

Match the molecular technique with the appropriate microorganism identification.
-Transcription-mediated amplification (TMA)

Accepted Answer

A) Cytomegalovirus (CMV) 
B) Mycobacterium tuberculosis 
A) Cytomegalovirus (CMV) 
B) Mycobacterium tuberculosis

Question 4

Multiplex PCR is limited by primer-primer interference.

Accepted Answer

A) True 
 B)False

Question 5

Clinical outcomes of PCR can lead to

Accepted Answer

A) detection of gene mutations that signify the early development of cancer. 
B) identification of viral DNA associated with specific cancers. 
C) detection of genetic mutations associated with various diseases. 
D) All of the above. 
A) detection of gene mutations that signify the early development of cancer. 
B) identification of viral DNA associated with specific cancers. 
C) detection of genetic mutations associated with various diseases. 
D) All of the above.

Question 6

Match the blotting protocol with the clinical application.
-Southern blot

Accepted Answer

A) RNA 
B) DNA 
C) Use of labeled antibodies specific for the protein of interest 
A) RNA 
B) DNA 
C) Use of labeled antibodies specific for the protein of interest

Question 7

DNA can be amplified by

Accepted Answer

A) in vitro amplification. 
B) cloning. 
C) synthesis of proteins. 
D) Both A and B. 
A) in vitro amplification. 
B) cloning. 
C) synthesis of proteins. 
D) Both A and B.

Question 8

Match the technique with an appropriate description (use each answer only once).
-Transcription-mediated amplification

Accepted Answer

A) This amplifies target nucleic acid without the use of a thermocycler. A double-stranded DNA fragment is created and becomes the target for exponential amplification. 
B) An isothermal assay that targets either DNA or RNA but generates RNA as its amplified product. 
C) Oligonucleotide pairs hybridize to target sequences within the gene or the cryptic plasmid. 
D) Only RNA is targeted for amplification. 
A) This amplifies target nucleic acid without the use of a thermocycler. A double-stranded DNA fragment is created and becomes the target for exponential amplification. 
B) An isothermal assay that targets either DNA or RNA but generates RNA as its amplified product. 
C) Oligonucleotide pairs hybridize to target sequences within the gene or the cryptic plasmid. 
D) Only RNA is targeted for amplification.

All the following are true in regard to the polymerase chain reaction (PCR) except

Match the technique with an appropriate description (use each answer only once). -Nucleic acid sequence amplification

Match the molecular technique with the appropriate microorganism identification. -Transcription-mediated amplification (TMA)

Multiplex PCR is limited by primer-primer interference.

Clinical outcomes of PCR can lead to

Match the blotting protocol with the clinical application. -Southern blot

DNA can be amplified by

Match the technique with an appropriate description (use each answer only once). -Transcription-mediated amplification

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Basic Serologic Laboratory Techniques

Rapid Testing

Precipitation and Particle Agglutination Methods

Electrophoresis Techniques

Labeling Techniques in Immunoassay

Flow Cytometry

Infectious Diseases: Traditional and Alternate Diagnostic Techniques

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Primary and Acquired Immune Deficiency Syndromes

Hypersensitivity Reactions

Immunoproliferative Disorders

Tolerance, Autoimmunity, and Autoimmune Diseases

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Transplantation: Hla, Solid Organ, and Hematopoietic Stem Cells

Tumor Immunology and Up-To-Date Applications of Next-Generation Sequencing

Primer on Vaccines

Filters

Exam 14: Molecular Laboratory Techniques

All the following are true in regard to the polymerase chain reaction (PCR) except

Match the technique with an appropriate description (use each answer only once). -Nucleic acid sequence amplification

Match the molecular technique with the appropriate microorganism identification. -Transcription-mediated amplification (TMA)

Multiplex PCR is limited by primer-primer interference.

Clinical outcomes of PCR can lead to

Match the blotting protocol with the clinical application. -Southern blot

DNA can be amplified by

Match the technique with an appropriate description (use each answer only once). -Transcription-mediated amplification

An Overview of Immunology

Antigens and Antibodies

Cells and Cellular Activities of the Immune System: Granulocytes and Mononuclear Cells

Cells and Cellular Activities of the Immune System: Lymphocytes and Plasma Cells

Soluble Mediators of the Immune System

Safety in the Immunology-Serology Laboratory

Quality Assurance and Quality Control

Basic Serologic Laboratory Techniques

Rapid Testing

Precipitation and Particle Agglutination Methods

Electrophoresis Techniques

Labeling Techniques in Immunoassay

Flow Cytometry

Infectious Diseases: Traditional and Alternate Diagnostic Techniques

Streptococcal Infections

Syphilis

Vector-Borne Diseases

Toxoplasmosis

Cytomegalovirus

Infectious Mononucleosis

Viral Hepatitis

Rubella and Rubeola Infections

Primary and Acquired Immune Deficiency Syndromes

Hypersensitivity Reactions

Immunoproliferative Disorders

Tolerance, Autoimmunity, and Autoimmune Diseases

Systemic Lupus Erythematosus

Rheumatoid Arthritis

Transplantation: Hla, Solid Organ, and Hematopoietic Stem Cells

Tumor Immunology and Up-To-Date Applications of Next-Generation Sequencing

Primer on Vaccines

Filters