Question 1

What has genetic engineering been used for?

Accepted Answer

A)  to create mice with altered genomes so we can model certain human diseases 
B)  to create plants that are more resistant to certain pests or herbicides 
C)  to produce a vaccine against hoof-and-mouth disease 
D)  to induce bacteria to mass-produce human insulin 
A)  to create mice with altered genomes so we can model certain human diseases 
B)  to create plants that are more resistant to certain pests or herbicides 
C)  to produce a vaccine against hoof-and-mouth disease 
D)  to induce bacteria to mass-produce human insulin

Question 2

Figure 14-1  
-According to Figure 14-1, what is the importance of the sticky ends?

Accepted Answer

A)  allowing recombination 
B)  allowing digestion 
C)  preventing digestion 
D)  preventing recombination 
A)  allowing recombination 
B)  allowing digestion 
C)  preventing digestion 
D)  preventing recombination

Question 3

What are the three essential pieces of DNA required for PCR?

Accepted Answer

A)  one primer and a template 
B)  two primers and a template 
C)  one primer and unknown DNA 
D)  two primers and unknown DNA 
A)  one primer and a template 
B)  two primers and a template 
C)  one primer and unknown DNA 
D)  two primers and unknown DNA

Question 4

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-You would use this method to determine the presence or absence of a particular 20 bp nucleotide pattern in a noncoding region of a genome.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR

Question 5

Which of the following can be determined by DNA fingerprinting?

Accepted Answer

A)  the sequence of human genome 
B)  the genome of human fingers 
C)  the species which a sample came from 
D)  the difference between two individuals of the same species 
A)  the sequence of human genome 
B)  the genome of human fingers 
C)  the species which a sample came from 
D)  the difference between two individuals of the same species

Question 6

Match each term with its definition.
-DNA hybridization

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 7

Match each term with the most appropriate macromolecule being studied or manipulate
-transgenics

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein. 
A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein.

Question 8

Which of the following distinguishes DNA cloning from gene cloning?

Accepted Answer

A)  DNA cloning is a method for producing a few copies of a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
B)  DNA cloning is a method for producing a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
C)  Gene cloning is a method for producing many copies of a piece of DNA. DNA cloning is cloning that involves a gene. 
D)  DNA cloning is a method for producing many copies of a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
A)  DNA cloning is a method for producing a few copies of a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
B)  DNA cloning is a method for producing a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
C)  Gene cloning is a method for producing many copies of a piece of DNA. DNA cloning is cloning that involves a gene. 
D)  DNA cloning is a method for producing many copies of a piece of DNA. Gene cloning is DNA cloning that involves a gene.

Question 9

Match each term with the most appropriate macromolecule being studied or manipulate
-polyacrylamide gel electrophoresis

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
D) protein. 
A) DNA 
B) mRNA/cDNA 
C) protein 
D) protein.

Question 10

In what way are basic and applied research studies of a cloned gene different?

Accepted Answer

A)  In basic research, a researcher wants to study the gene's function, including how its expression is regulated, and the nature of the gene's product, while in applied research, the interest is in using cloned genes for private application. 
B)  In basic research, a researcher does not want to study the gene's function, including how its expression is regulated, and the nature of the gene's product, while in applied research, the interest is in using cloned genes for medical application. 
C)  In basic research, a researcher does not learn about the gene's structure, including its DNA sequence and sequences that regulate its expression, while in applied research, the interest is in the structure and function of a gene. 
D)  In basic research, a researcher learns about the gene's structure, including its DNA sequence and sequences that regulate its expression, while in applied research, the interest is not in the structure and function of a gene. 
A)  In basic research, a researcher wants to study the gene's function, including how its expression is regulated, and the nature of the gene's product, while in applied research, the interest is in using cloned genes for private application. 
B)  In basic research, a researcher does not want to study the gene's function, including how its expression is regulated, and the nature of the gene's product, while in applied research, the interest is in using cloned genes for medical application. 
C)  In basic research, a researcher does not learn about the gene's structure, including its DNA sequence and sequences that regulate its expression, while in applied research, the interest is in the structure and function of a gene. 
D)  In basic research, a researcher learns about the gene's structure, including its DNA sequence and sequences that regulate its expression, while in applied research, the interest is not in the structure and function of a gene.

Question 11

Match each term with its definition.
-genomic library

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Exam 14: Dna Technologies

What has genetic engineering been used for?

Figure 14-1 -According to Figure 14-1, what is the importance of the sticky ends?

What are the three essential pieces of DNA required for PCR?

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -You would use this method to determine the presence or absence of a particular 20 bp nucleotide pattern in a noncoding region of a genome.

Which of the following can be determined by DNA fingerprinting?

Match each term with its definition. -DNA hybridization

Match each term with the most appropriate macromolecule being studied or manipulate -transgenics

Which of the following distinguishes DNA cloning from gene cloning?

Match each term with the most appropriate macromolecule being studied or manipulate -polyacrylamide gel electrophoresis

In what way are basic and applied research studies of a cloned gene different?

Match each term with its definition. -genomic library

Light and Life

The Cell: an Overview

Energy and Enzymes

Cell Membranes and Signaling

Cellular Respiration

Photosynthesis

Cell Cycles

Genetic Recombination

The Chromosomal Basis of Mendelian Inheritance

Genetic Linkage, Sex-Linkage, and Other Non-Mendelian Inheritance Mechanisms

DNA Structure, Replication, and Repair

Gene Structure Expression, and Mutation

Regulation of Gene Expression

Genomes

Evolution: the Development of the Theory

Microevolution: Changes Within Populations

Speciation and Macroevolution

Systematics and Phylogenetics: Revealing the Tree of Life

Humans and Evolution

Defining Life and Its Origins

Viruses, Viroids, and Prions: Infectious Biological Particles

Bacteria and Archaea

Protists

Fungi

Plants

Animals

Conservation of Biodiversity

Population Ecology

Species Interactions and Community Ecology

Ecosystems

Animal Behaviour

Organization of the Plant Body

Transport in Plants

Reproduction and Development in Flowering Plants

Plant Nutrition

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Animal Nutrition

Gas Exchange: the Respiratory System

Internal Transport: the Circulatory System

Regulation of the Internal Environment: Water, Solutes, and Temperature

Control of Animal Processes: Endocrine Control

Animal Reproduction

Control of Animal Processes: Neural Control

Muscles, Skeletons, and Body Movements

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