Question 1

Match each term with the most appropriate macromolecule being studied or manipulate
-sequencing

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein. 
A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein.

Question 2

DNA fingerprinting is limited to human studies.

Accepted Answer

A) True 
 B)False

Question 3

Match each term with its definition.
-genetic engineering

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 4

Match each term with its definition.
-recombinant DNA

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 5

Match each term with its definition.
-Southern blot

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 6

cDNA library is the partial collection of cloned cDNAs made from the mRNAs isolated from a cell.

Accepted Answer

A) True 
 B)False

Question 7

Match each term with its definition.
-functional genomics

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 8

Match each term with its definition.
-PCR

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 9

Match each term with the most appropriate macromolecule being studied or manipulate
-blotting

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein. 
A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein.

Question 10

In which stage is the foreign gene introduced into the developing embryo during the creation of transgenic animals?

Accepted Answer

A)  at the blastocyst stage, via a transgenic cell 
B)  in cell culture, prior to fertilization 
C)  at the sperm stage, via direct microinjection of the new DNA 
D)  at the egg stage, via direct microinjection of the new DNA 
A)  at the blastocyst stage, via a transgenic cell 
B)  in cell culture, prior to fertilization 
C)  at the sperm stage, via direct microinjection of the new DNA 
D)  at the egg stage, via direct microinjection of the new DNA

Question 11

Why is X-gal used in cloning medium?

Accepted Answer

A)  It distinguishes between bacteria that have been transformed with recombinant plasmids and nonrecombinant plasmids by black-white screening. 
B)  It distinguishes between bacteria that have been transformed with recombinant plasmids and nonrecombinant plasmids by blue-white screening. 
C)  It distinguishes between plasmids that have been transformed and those that have not by blue-white screening. 
D)  It distinguishes between plasmids that have been transformed and those that have not by black-white screening. 
A)  It distinguishes between bacteria that have been transformed with recombinant plasmids and nonrecombinant plasmids by black-white screening. 
B)  It distinguishes between bacteria that have been transformed with recombinant plasmids and nonrecombinant plasmids by blue-white screening. 
C)  It distinguishes between plasmids that have been transformed and those that have not by blue-white screening. 
D)  It distinguishes between plasmids that have been transformed and those that have not by black-white screening.

Question 12

Explain the ethical concerns with germ-line gene therapy in humans.

Accepted Answer

If gene therapy were applied to germ-lin

Question 13

Which sequence presents the three stages of a PCR in the correct order?

Accepted Answer

A)  annealing 
B)  extension 
C)  denaturation 
D)  denaturation. 
A)  annealing 
B)  extension 
C)  denaturation 
D)  denaturation.

Question 14

Match each term with its definition.
-proteomics

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 15

Thousands of different restriction enzymes have been identified that can cut DNA at a specific restriction site.

Accepted Answer

A) True 
 B)False

Question 16

Match the names of the processes with the descriptions.
-This is the technique that would be used by a forensic scientist trying to determine if a suspect is the actual rapist.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 17

Match the names of the processes with the descriptions.
-This is the methodology that would be considered by a scientist who wants to generate mice that express a fluorescent protein in every cell of their body.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 18

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-You would use this to cut DNA at a selected site.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR

Question 19

Different methodologies can often be used to obtain the same information about a DNA sequence.

Accepted Answer

A) True 
 B)False

Question 20

Why would researchers want to have both genomic libraries and cDNA libraries from a particular organism?

Accepted Answer

A)  because a cDNA library helps us understand how retroviruses work, but a genomic library does not 
B)  because a genomic library is generally too big to be helpful, while a cDNA library, being smaller, is much easier to study 
C)  because a genomic library will be the same in every cell, while a cDNA library tells researchers which genes are being expressed in individual cell types 
D)  because a cDNA library is a lot easier to clone than a genomic library 
A)  because a cDNA library helps us understand how retroviruses work, but a genomic library does not 
B)  because a genomic library is generally too big to be helpful, while a cDNA library, being smaller, is much easier to study 
C)  because a genomic library will be the same in every cell, while a cDNA library tells researchers which genes are being expressed in individual cell types 
D)  because a cDNA library is a lot easier to clone than a genomic library

Exam 14: Dna Technologies

Match each term with the most appropriate macromolecule being studied or manipulate -sequencing

DNA fingerprinting is limited to human studies.

Match each term with its definition. -genetic engineering

Match each term with its definition. -recombinant DNA

Match each term with its definition. -Southern blot

cDNA library is the partial collection of cloned cDNAs made from the mRNAs isolated from a cell.

Match each term with its definition. -functional genomics

Match each term with its definition. -PCR

Match each term with the most appropriate macromolecule being studied or manipulate -blotting

In which stage is the foreign gene introduced into the developing embryo during the creation of transgenic animals?

Why is X-gal used in cloning medium?

Explain the ethical concerns with germ-line gene therapy in humans.

Which sequence presents the three stages of a PCR in the correct order?

Match each term with its definition. -proteomics

Thousands of different restriction enzymes have been identified that can cut DNA at a specific restriction site.

Match the names of the processes with the descriptions. -This is the technique that would be used by a forensic scientist trying to determine if a suspect is the actual rapist.

Match the names of the processes with the descriptions. -This is the methodology that would be considered by a scientist who wants to generate mice that express a fluorescent protein in every cell of their body.

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -You would use this to cut DNA at a selected site.

Different methodologies can often be used to obtain the same information about a DNA sequence.

Why would researchers want to have both genomic libraries and cDNA libraries from a particular organism?

Light and Life

The Cell: an Overview

Energy and Enzymes

Cell Membranes and Signaling

Cellular Respiration

Photosynthesis

Cell Cycles

Genetic Recombination

The Chromosomal Basis of Mendelian Inheritance

Genetic Linkage, Sex-Linkage, and Other Non-Mendelian Inheritance Mechanisms

DNA Structure, Replication, and Repair

Gene Structure Expression, and Mutation

Regulation of Gene Expression

Genomes

Evolution: the Development of the Theory

Microevolution: Changes Within Populations

Speciation and Macroevolution

Systematics and Phylogenetics: Revealing the Tree of Life

Humans and Evolution

Defining Life and Its Origins

Viruses, Viroids, and Prions: Infectious Biological Particles

Bacteria and Archaea

Protists

Fungi

Plants

Animals

Conservation of Biodiversity

Population Ecology

Species Interactions and Community Ecology

Ecosystems

Animal Behaviour

Organization of the Plant Body

Transport in Plants

Reproduction and Development in Flowering Plants

Plant Nutrition

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Animal Nutrition

Gas Exchange: the Respiratory System

Internal Transport: the Circulatory System

Regulation of the Internal Environment: Water, Solutes, and Temperature

Control of Animal Processes: Endocrine Control

Animal Reproduction

Control of Animal Processes: Neural Control

Muscles, Skeletons, and Body Movements

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