Question 1

Match each term with its definition.
-structural genomics

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 2

Which approach would be most helpful in determining whether the gene for a particular protein is the same length in frogs, humans, and trees?

Accepted Answer

A)  use cDNA library construction and agarose gel electrophoresis 
B)  use genomic library construction and PCR 
C)  use DNA cloning and DNA hybridization 
D)  use PCR and agarose gel electrophoresis 
A)  use cDNA library construction and agarose gel electrophoresis 
B)  use genomic library construction and PCR 
C)  use DNA cloning and DNA hybridization 
D)  use PCR and agarose gel electrophoresis

Question 3

Sticky ends are ends on DNA fragments that are complementary to the DNA ends on the open plasmid.

Accepted Answer

A) True 
 B)False

Question 4

If you were to utilize "gene machine," which of the following would you be using?

Accepted Answer

A)  a machine that can create DNA sequences of choice by automated chemical synthesis in the laboratory using a RNA synthesizer responding to computer input 
B)  a machine that can create DNA sequences of choice by automated chemical synthesis in the laboratory using a DNA synthesizer responding to computer input 
C)  a machine that can create DNA sequences of choice by manual chemical synthesis in the laboratory using a DNA synthesizer responding to computer input 
D)  a machine that can create RNA sequences of choice by automated chemical synthesis in the laboratory using a RNA synthesizer responding to computer input 
A)  a machine that can create DNA sequences of choice by automated chemical synthesis in the laboratory using a RNA synthesizer responding to computer input 
B)  a machine that can create DNA sequences of choice by automated chemical synthesis in the laboratory using a DNA synthesizer responding to computer input 
C)  a machine that can create DNA sequences of choice by manual chemical synthesis in the laboratory using a DNA synthesizer responding to computer input 
D)  a machine that can create RNA sequences of choice by automated chemical synthesis in the laboratory using a RNA synthesizer responding to computer input

Question 5

Make an argument for which molecular biology technique discussed in this chapter is the single most important technique for forensic scientists and justify your answer with the benefits of this method compared to the others that are available to you.

Accepted Answer

In forensic science, sample sizes are ve

Question 6

Match each term with its definition.
-restriction endonucleases

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 7

What is the natural function of restriction endonucleases?

Accepted Answer

A)  regulating bacterial gene expression 
B)  DNA recombination in vivo 
C)  defending against viruses that infect bacteria 
D)  DNA manipulation in vitro 
A)  regulating bacterial gene expression 
B)  DNA recombination in vivo 
C)  defending against viruses that infect bacteria 
D)  DNA manipulation in vitro

Question 8

Match the names of the processes with the descriptions.
-This is any technique applied to living organisms that modifies or makes them for a specific purpose.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 9

What is the natural function of the T DNA contained within the Ti plasmid?

Accepted Answer

A)  to increase diversity 
B)  to induce tumours 
C)  to allow for recombination 
D)  to transfer genes 
A)  to increase diversity 
B)  to induce tumours 
C)  to allow for recombination 
D)  to transfer genes

Question 10

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-You would use this method to mass-produce a gene in a bacterial cell that does not normally have this gene.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR

Question 11

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-You could use this technique to see if a DNA sequence is present in an individual's genome without having to use Sanger sequencing.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR

Question 12

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-This technique will let you move a gene into a vector for expression in different cells.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR

Question 13

Match each term with its definition.
-agarose gel electrophoresis

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 14

How is the Ti plasmid used to create transgenic plants?

Accepted Answer

A)  The Ti plasmid inserts the foreign gene directly into the plant's nuclear DNA. 
B)  Once the Rhizobium radiobacter forms a tumour in the plant, the tumour cells can take up the foreign DNA and incorporate it into their chromosomes. 
C)  The Ti plasmid allows all of the plant cells to host the bacteria Rhizobium radiobacter and thus express the foreign gene that was inserted into the bacterial chromosome. 
D)  The Ti plasmid inserts the foreign DNA into the DNA in the chloroplast. 
A)  The Ti plasmid inserts the foreign gene directly into the plant's nuclear DNA. 
B)  Once the Rhizobium radiobacter forms a tumour in the plant, the tumour cells can take up the foreign DNA and incorporate it into their chromosomes. 
C)  The Ti plasmid allows all of the plant cells to host the bacteria Rhizobium radiobacter and thus express the foreign gene that was inserted into the bacterial chromosome. 
D)  The Ti plasmid inserts the foreign DNA into the DNA in the chloroplast.

Question 15

Match each term with the most appropriate macromolecule being studied or manipulate
-PCR

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein. 
A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein.

Question 16

Match each term with its definition.
-DNA fingerprinting

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 17

What do restriction endonucleases do?

Accepted Answer

A)  break glycosidic bonds 
B)  break ester bonds 
C)  break phosphodiester bonds 
D)  break peptide bonds 
A)  break glycosidic bonds 
B)  break ester bonds 
C)  break phosphodiester bonds 
D)  break peptide bonds

Question 18

What are restriction fragment length polymorphisms used for?

Accepted Answer

A)  to prepare DNA for further sequence analysis 
B)  to treat sickle cell anemia 
C)  to test the effectiveness of different restriction enzymes on a sequence of DNA 
D)  to compare DNA from two individuals 
A)  to prepare DNA for further sequence analysis 
B)  to treat sickle cell anemia 
C)  to test the effectiveness of different restriction enzymes on a sequence of DNA 
D)  to compare DNA from two individuals

Question 19

Match each term with its definition.
-cDNA library

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 20

Match each term with the most appropriate macromolecule being studied or manipulate
-cDNA library

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein. 
D)  protein 
A) DNA 
B) mRNA/cDNA 
C) protein. 
D)  protein

Exam 14: Dna Technologies

Match each term with its definition. -structural genomics

Which approach would be most helpful in determining whether the gene for a particular protein is the same length in frogs, humans, and trees?

Sticky ends are ends on DNA fragments that are complementary to the DNA ends on the open plasmid.

If you were to utilize "gene machine," which of the following would you be using?

Make an argument for which molecular biology technique discussed in this chapter is the single most important technique for forensic scientists and justify your answer with the benefits of this method compared to the others that are available to you.

Match each term with its definition. -restriction endonucleases

What is the natural function of restriction endonucleases?

Match the names of the processes with the descriptions. -This is any technique applied to living organisms that modifies or makes them for a specific purpose.

What is the natural function of the T DNA contained within the Ti plasmid?

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -You would use this method to mass-produce a gene in a bacterial cell that does not normally have this gene.

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -You could use this technique to see if a DNA sequence is present in an individual's genome without having to use Sanger sequencing.

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -This technique will let you move a gene into a vector for expression in different cells.

Match each term with its definition. -agarose gel electrophoresis

How is the Ti plasmid used to create transgenic plants?

Match each term with the most appropriate macromolecule being studied or manipulate -PCR

Match each term with its definition. -DNA fingerprinting

What do restriction endonucleases do?

What are restriction fragment length polymorphisms used for?

Match each term with its definition. -cDNA library

Match each term with the most appropriate macromolecule being studied or manipulate -cDNA library

Light and Life

The Cell: an Overview

Energy and Enzymes

Cell Membranes and Signaling

Cellular Respiration

Photosynthesis

Cell Cycles

Genetic Recombination

The Chromosomal Basis of Mendelian Inheritance

Genetic Linkage, Sex-Linkage, and Other Non-Mendelian Inheritance Mechanisms

DNA Structure, Replication, and Repair

Gene Structure Expression, and Mutation

Regulation of Gene Expression

Genomes

Evolution: the Development of the Theory

Microevolution: Changes Within Populations

Speciation and Macroevolution

Systematics and Phylogenetics: Revealing the Tree of Life

Humans and Evolution

Defining Life and Its Origins

Viruses, Viroids, and Prions: Infectious Biological Particles

Bacteria and Archaea

Protists

Fungi

Plants

Animals

Conservation of Biodiversity

Population Ecology

Species Interactions and Community Ecology

Ecosystems

Animal Behaviour

Organization of the Plant Body

Transport in Plants

Reproduction and Development in Flowering Plants

Plant Nutrition

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Animal Nutrition

Gas Exchange: the Respiratory System

Internal Transport: the Circulatory System

Regulation of the Internal Environment: Water, Solutes, and Temperature

Control of Animal Processes: Endocrine Control

Animal Reproduction

Control of Animal Processes: Neural Control

Muscles, Skeletons, and Body Movements

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