Question 1

Manufactures a 10-12 base segment of RNA.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 2

Which of the following is a restriction placed on DNA polymerase?

Accepted Answer

A)  DNA polymerase can attach new nucleotides only in the 5' to 3' direction. 
B)  DNA polymerases must begin synthesis using an RNA primer. 
C)  DNA polymerases must have a template strand to copy from. 
D)  All of the above are restrictions of DNA polymerase. 
A)  DNA polymerase can attach new nucleotides only in the 5' to 3' direction. 
B)  DNA polymerases must begin synthesis using an RNA primer. 
C)  DNA polymerases must have a template strand to copy from. 
D)  All of the above are restrictions of DNA polymerase.

Question 3

DNA polymerases are unable to bind to what areas of the chromosome?

Accepted Answer

A)  Centromeres 
B)  3' end of telomeres 
C)  Origins of replication 
A)  Centromeres 
B)  3' end of telomeres 
C)  Origins of replication

Question 4

Synthesizes the lagging strand of the DNA.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 5

Okazaki fragments do which of the following?

Accepted Answer

A)  Assist in forming the replication fork 
B)  Bind to the oriC region 
C)  Assist in the synthesis of DNA from the lagging strand 
D)  Reform the double-helix following replication 
E)  None of the answers are correct 
A)  Assist in forming the replication fork 
B)  Bind to the oriC region 
C)  Assist in the synthesis of DNA from the lagging strand 
D)  Reform the double-helix following replication 
E)  None of the answers are correct

Question 6

Which of the following is NOT a reason for the high fidelity of the newly synthesized DNA molecule?

Accepted Answer

A)  The hydrogen bonding between purines and pyrimidines is stable. 
B)  The DNA polymerase is unlikely to form bonds between nucleotides if they are mismatched. 
C)  The DNA polymerase has exonuclease functions. 
D)  The DNA polymerase has the ability to change the structure of the base in order to form the correct bond. 
A)  The hydrogen bonding between purines and pyrimidines is stable. 
B)  The DNA polymerase is unlikely to form bonds between nucleotides if they are mismatched. 
C)  The DNA polymerase has exonuclease functions. 
D)  The DNA polymerase has the ability to change the structure of the base in order to form the correct bond.

Question 7

The movement of the replication fork in bacterial replication is unidirectional.

Accepted Answer

A) True 
 B)False

Question 8

The ability of the DNA polymerase to remove mismatched bases is called exonuclease cleavage
(proofreading).

Accepted Answer

A) True 
 B)False

Question 9

The Meselson-Stahl experiments supported the model of dispersive DNA replication.

Accepted Answer

A) True 
 B)False

Question 10

Responsible for the majority of DNA replication.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 11

Replication usually begins in GC rich regions due to the presence of only 2 hydrogen bonds between
the bases.

Accepted Answer

A) True 
 B)False

Question 12

What was the control in Arthur Kornberg's in vitro replication experiment described in this chapter?

Accepted Answer

A)  The use of radiolabeled nucleotide triphosphates 
B)  The exclusion of template DNA in one test tube 
C)  Adding perchloric acid 
D)  The use of 
E)  coli proteins 
A)  The use of radiolabeled nucleotide triphosphates 
B)  The exclusion of template DNA in one test tube 
C)  Adding perchloric acid 
D)  The use of 
E)  coli proteins

Question 13

Prevents supercoiling ahead the replication fork.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 14

What functions are accomplished by the primosome?

Accepted Answer

A)  Tracking along DNA 
B)  Tracking along DNA, separating double stranded DNA 
C)  Tracking along DNA, separating double stranded DNA, synthesizing RNA primers 
D)  Tracking along DNA, separating double stranded DNA, synthesizing RNA primers, adding nucleotides 
A)  Tracking along DNA 
B)  Tracking along DNA, separating double stranded DNA 
C)  Tracking along DNA, separating double stranded DNA, synthesizing RNA primers 
D)  Tracking along DNA, separating double stranded DNA, synthesizing RNA primers, adding nucleotides

Question 15

Which of the following best describes the double-helix of DNA?

Accepted Answer

A)  It has directionality 
B)  The strands are arranged in an anti-parallel arrangement 
C)  The strands are complementary 
D)  All of the answers are correct 
A)  It has directionality 
B)  The strands are arranged in an anti-parallel arrangement 
C)  The strands are complementary 
D)  All of the answers are correct

Question 16

Which of the following stops the replication of DNA in prokaryotes?

Accepted Answer

A)  Tus proteins 
B)  DNA ligase 
C)  Okazaki fragments 
D)  The end of the chromosome 
A)  Tus proteins 
B)  DNA ligase 
C)  Okazaki fragments 
D)  The end of the chromosome

Question 17

The origin of replication in bacteria is called OriC.
TRUE

Accepted Answer

A) True 
 B)False

Question 18

DNA helicase enzymes move in what direction along the DNA during DNA replication?

Accepted Answer

A)  5' to 3' 
B)  3' to 5' 
C)  They remain stationary 
A)  5' to 3' 
B)  3' to 5' 
C)  They remain stationary

Question 19

DNA polymerase III has an error in replication once every 100 million nucleotides.

Accepted Answer

A) True 
 B)False

Question 20

DNA polymerase III is the main replication enzyme in prokaryotes. What is it in eukaryotes?

Accepted Answer

A)  DNA pol a 
B)  DNA pol d 
C)  DNA pol b 
D)  DNA pol g 
A)  DNA pol a 
B)  DNA pol d 
C)  DNA pol b 
D)  DNA pol g

Exam 11: Dna Replication

Manufactures a 10-12 base segment of RNA.

Which of the following is a restriction placed on DNA polymerase?

DNA polymerases are unable to bind to what areas of the chromosome?

Synthesizes the lagging strand of the DNA.

Okazaki fragments do which of the following?

Which of the following is NOT a reason for the high fidelity of the newly synthesized DNA molecule?

The movement of the replication fork in bacterial replication is unidirectional.

The ability of the DNA polymerase to remove mismatched bases is called exonuclease cleavage (proofreading).

The Meselson-Stahl experiments supported the model of dispersive DNA replication.

Responsible for the majority of DNA replication.

Replication usually begins in GC rich regions due to the presence of only 2 hydrogen bonds between the bases.

What was the control in Arthur Kornberg's in vitro replication experiment described in this chapter?

Prevents supercoiling ahead the replication fork.

What functions are accomplished by the primosome?

Which of the following best describes the double-helix of DNA?

Which of the following stops the replication of DNA in prokaryotes?

The origin of replication in bacteria is called OriC. TRUE

DNA helicase enzymes move in what direction along the DNA during DNA replication?

DNA polymerase III has an error in replication once every 100 million nucleotides.

DNA polymerase III is the main replication enzyme in prokaryotes. What is it in eukaryotes?

Overview of Genetics

Mendelian Inheritance

Reproduction and Chromosome Transmission

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria and Bacteriophages

Gene Regulation in Eukaryotes

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular

Recombinant Dna Technology

Biotechnology

Genomics I: Analysis of Dna

Genomics II: Functional Genomics, Proteomics, and

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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