Question 1

The regulation of the CAP complex using cAMP is an example of inducible genetic regulation.

Accepted Answer

A) True 
 B)False

Question 2

If a bacteria is placed in an environment that contains both glucose and lactose, the regulation of the lac operon will allow which nutrient to be processed first?

Accepted Answer

A)  Glucose 
B)  Lactose 
C)  Both will be processed equally 
D)  Neither will be processed in this environment 
A)  Glucose 
B)  Lactose 
C)  Both will be processed equally 
D)  Neither will be processed in this environment

Question 3

Which of the following is found in an operon?

Accepted Answer

A)  Promoter 
B)  Terminator 
C)  Two or more genes 
D)  Operator 
E)  All of the answers are correct 
A)  Promoter 
B)  Terminator 
C)  Two or more genes 
D)  Operator 
E)  All of the answers are correct

Question 4

A gene is inducible and under negative control. Which of the following pairs will allow expression of this gene?

Accepted Answer

A)  Activator + repressor 
B)  Activator + inhibitor 
C)  Repressor + inducer 
D)  Repressor + co-repressor 
A)  Activator + repressor 
B)  Activator + inhibitor 
C)  Repressor + inducer 
D)  Repressor + co-repressor

Question 5

Allosteric regulation is accomplished by:

Accepted Answer

A)  A small molecule that fits into an enzyme's active site 
B)  A large protein that blocks an enzyme's active site 
C)  A small molecule that fits into a site on the enzyme that is not the active site 
D)  A small molecule that covalently modifies a site on the enzyme that is not the active site 
A)  A small molecule that fits into an enzyme's active site 
B)  A large protein that blocks an enzyme's active site 
C)  A small molecule that fits into a site on the enzyme that is not the active site 
D)  A small molecule that covalently modifies a site on the enzyme that is not the active site

Question 6

What stem-loop conformations favor attenuation?

Accepted Answer

A)  1-2 
B)  1-2 and 2-3 
C)  2-3 
D)  1-2 and 3-4 
A)  1-2 
B)  1-2 and 2-3 
C)  2-3 
D)  1-2 and 3-4

Question 7

In the same experiment, what would have been the conclusion if all four tubes produced a yellow color when b-ONPG was added?

Accepted Answer

A)  Expression of the lac operon is constitutive whether lacI is functional or not 
B)  LacI provides the binding site for the repressor 
C)  LacI encodes a diffusible repressor 
D)  The researcher added too much b-ONPG 
A)  Expression of the lac operon is constitutive whether lacI is functional or not 
B)  LacI provides the binding site for the repressor 
C)  LacI encodes a diffusible repressor 
D)  The researcher added too much b-ONPG

Question 8

A bacterium is transformed with two plasmids, both containing the same gene. In one, the gene is placed next to a strong promoter and in the other, a weak promoter. How would you expect the two transformed bacteria to grow?

Accepted Answer

A)  The bacteria with the strong promoter would grow slower. 
B)  The bacteria with the weak promoter would grow slower. 
C)  Neither bacteria would grow. 
D)  Both bacteria would grow at the same rate. 
A)  The bacteria with the strong promoter would grow slower. 
B)  The bacteria with the weak promoter would grow slower. 
C)  Neither bacteria would grow. 
D)  Both bacteria would grow at the same rate.

Question 9

Which cycle would prevail if there were a mutation in the promoter PRE?

Accepted Answer

A)  Lysogenic 
B)  Lytic 
C)  Neither 
A)  Lysogenic 
B)  Lytic 
C)  Neither

Question 10

If CAP could not bind to its CAP site, then what would be the result? Assume lactose is present in each scenario.

Accepted Answer

A)  Transcription would be difficult to repress in the presence of glucose. 
B)  Transcription would be difficult to activate in the presence of glucose 
C)  Transcription would be difficult to activate in the absence of glucose 
D)  Transcription would be difficult to repress in the presence of glucose 
A)  Transcription would be difficult to repress in the presence of glucose. 
B)  Transcription would be difficult to activate in the presence of glucose 
C)  Transcription would be difficult to activate in the absence of glucose 
D)  Transcription would be difficult to repress in the presence of glucose

Question 11

The term enzyme adaptation is used to describe an enzyme that appears in a living cell following
exposure to a specific substrate.

Accepted Answer

A) True 
 B)False

Question 12

In the lac operon, the operator site is recognized by a activator protein.

Accepted Answer

A) True 
 B)False

Question 13

The form of regulation that involves a physical change in the shape of an enzyme is called allosteric
regulation.

Accepted Answer

A) True 
 B)False

Question 14

Repressor proteins are responsible for negative transcriptional regulation.

Accepted Answer

A) True 
 B)False

Question 15

Which of the following is not associated with positive transcriptional regulation?

Accepted Answer

A)  Activators 
B)  Inducers 
C)  Corepressors 
D)  All of the answers are involved in positive regulation 
A)  Activators 
B)  Inducers 
C)  Corepressors 
D)  All of the answers are involved in positive regulation

Question 16

The regulation of protein function, not gene expression is called regulation.

Accepted Answer

A)  Posttranslational 
B)  Transcriptional 
C)  Translational 
D)  Post-transcriptional 
A)  Posttranslational 
B)  Transcriptional 
C)  Translational 
D)  Post-transcriptional

Exam 14: Gene Regulation in Bacteria and Bacteriophages

The regulation of the CAP complex using cAMP is an example of inducible genetic regulation.

If a bacteria is placed in an environment that contains both glucose and lactose, the regulation of the lac operon will allow which nutrient to be processed first?

Which of the following is found in an operon?

A gene is inducible and under negative control. Which of the following pairs will allow expression of this gene?

Allosteric regulation is accomplished by:

What stem-loop conformations favor attenuation?

In the same experiment, what would have been the conclusion if all four tubes produced a yellow color when b-ONPG was added?

A bacterium is transformed with two plasmids, both containing the same gene. In one, the gene is placed next to a strong promoter and in the other, a weak promoter. How would you expect the two transformed bacteria to grow?

Which cycle would prevail if there were a mutation in the promoter PRE?

If CAP could not bind to its CAP site, then what would be the result? Assume lactose is present in each scenario.

The term enzyme adaptation is used to describe an enzyme that appears in a living cell following exposure to a specific substrate.

In the lac operon, the operator site is recognized by a activator protein.

The form of regulation that involves a physical change in the shape of an enzyme is called allosteric regulation.

Repressor proteins are responsible for negative transcriptional regulation.

Which of the following is not associated with positive transcriptional regulation?

The regulation of protein function, not gene expression is called regulation.

Overview of Genetics

Mendelian Inheritance

Reproduction and Chromosome Transmission

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Eukaryotes

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular

Recombinant Dna Technology

Biotechnology

Genomics I: Analysis of Dna

Genomics II: Functional Genomics, Proteomics, and

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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