Question 1

A special vector that is designed to clone the coding sequence of a gene correctly is called a structural
vector.

Accepted Answer

A) True 
 B)False

Question 2

RT-PCR is used for which of the following?

Accepted Answer

A)  Conduct a PCR reaction without the use of primers 
B)  Generate cDNA 
C)  Quantify RNA in a cell 
D)  Nonspecific DNA amplification 
E)  None of the answers are correct 
A)  Conduct a PCR reaction without the use of primers 
B)  Generate cDNA 
C)  Quantify RNA in a cell 
D)  Nonspecific DNA amplification 
E)  None of the answers are correct

Question 3

Which of the following individual(s) developed the process of the polymerase chain reaction?

Accepted Answer

A)  Watson and Crick 
B)  McClintock 
C)  Mullis 
D)  Pauling 
E)  None of the answers are correct 
A)  Watson and Crick 
B)  McClintock 
C)  Mullis 
D)  Pauling 
E)  None of the answers are correct

Question 4

Which of the following would a competent cell be able to do?

Accepted Answer

A)  Resist transfection by a viral vector 
B)  Repair DNA strands without the aid of DNA lygase 
C)  Utilize transposons as a vector 
D)  Take up DNA from the external environment 
E)  None of the answers are correct 
A)  Resist transfection by a viral vector 
B)  Repair DNA strands without the aid of DNA lygase 
C)  Utilize transposons as a vector 
D)  Take up DNA from the external environment 
E)  None of the answers are correct

Question 5

In a cloning experiment, you use a vector that contains a lacZ gene as a selectable marker. If the competent cells are grown on X-Gal and IPTG, which colonies would contain chromosomal DNA?

Accepted Answer

A)  The white colonies 
B)  The blue colonies 
C)  Half the total colonies 
D)  None of the colonies 
A)  The white colonies 
B)  The blue colonies 
C)  Half the total colonies 
D)  None of the colonies

Question 6

A plasmid that contains separate origins of replication for two different species is called a .

Accepted Answer

A)  R factor 
B)  Expression vector 
C)  Viral vector 
D)  Shuttle vector 
E)  None of the answers are correct 
A)  R factor 
B)  Expression vector 
C)  Viral vector 
D)  Shuttle vector 
E)  None of the answers are correct

Question 7

A dideoxy nucleotide is missing an -OH group at the 2' end.

Accepted Answer

A) True 
 B)False

Question 8

Cloned genes may be mutated outside of a cell by a process called in vitro site-directed mutagensis.

Accepted Answer

A) True 
 B)False

Question 9

The use of a radioactive probe to screen a DNA library for a gene of interest is called colony
hybridization.

Accepted Answer

A) True 
 B)False

Question 10

What is the origin of restriction endonucleases?

Accepted Answer

A)  They are part of DNA repair mechanisms in eukaryotic cells 
B)  They are a defense mechanism against viruses in bacteria 
C)  They are replication enzymes of yeast 
D)  They are transposable elements of Drosophila 
A)  They are part of DNA repair mechanisms in eukaryotic cells 
B)  They are a defense mechanism against viruses in bacteria 
C)  They are replication enzymes of yeast 
D)  They are transposable elements of Drosophila

Question 11

cDNA is made using what as the starting material?

Accepted Answer

A)  Plasmid vectors 
B)  Viral DNA 
C)  Chromosomal DNA 
D)  RNA 
A)  Plasmid vectors 
B)  Viral DNA 
C)  Chromosomal DNA 
D)  RNA

Question 12

The isolation and copying of a gene, usually in large quantities is called gene cloning.

Accepted Answer

A) True 
 B)False

Question 13

Which of the following is an example of a palindromic DNA sequence?

Accepted Answer

A)  5' - ATCGAC - 3' 3' - TAGCAG - 5' 
B)  5' - ATCATC - 3' 3' - ATCATC - 5' 
C)  5' - GCCGCC - 3' 3' - CGGCGG - 5' 
D)  5' - CTGCAG - 3' 3' - GACGTC - 5' 
A)  5' - ATCGAC - 3' 3' - TAGCAG - 5' 
B)  5' - ATCATC - 3' 3' - ATCATC - 5' 
C)  5' - GCCGCC - 3' 3' - CGGCGG - 5' 
D)  5' - CTGCAG - 3' 3' - GACGTC - 5'

Question 14

What is a DNA library?

Accepted Answer

A)  A collection of vectors, each containing a fragment of the original genome 
B)  A complete sequence of the genetic material in an organism 
C)  An electronic file that may be used for additional genetic analysis 
D)  A collection of mRNA from a given cell 
E)  None of the answers are correct 
A)  A collection of vectors, each containing a fragment of the original genome 
B)  A complete sequence of the genetic material in an organism 
C)  An electronic file that may be used for additional genetic analysis 
D)  A collection of mRNA from a given cell 
E)  None of the answers are correct

Question 15

R factors typically contain genes for antibiotic resistance.

Accepted Answer

A) True 
 B)False

Question 16

Transfection is when a bacterial vector is taken up into the host cell.

Accepted Answer

A) True 
 B)False

Question 17

Southern blotting may be used for which of the following?

Accepted Answer

A)  Identify gene families 
B)  Detect the number of copies of a gene in the genome 
C)  Identify deletions 
D)  Identify homologous genes in different species 
E)  All of the answers are correct 
A)  Identify gene families 
B)  Detect the number of copies of a gene in the genome 
C)  Identify deletions 
D)  Identify homologous genes in different species 
E)  All of the answers are correct

Question 18

Restriction endonucleases are specific in the DNA sequences that they recognize.

Accepted Answer

A) True 
 B)False

Question 19

The polymerase used in PCR reactions is derived from the thermophile organism Thermus aquaticus.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following is used to identify a specific DNA sequence in a library?

Accepted Answer

A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting 
A)  Western blotting 
B)  Northern blotting 
C)  Southern blotting 
D)  Eastern blotting

Exam 18: Recombinant Dna Technology

A special vector that is designed to clone the coding sequence of a gene correctly is called a structural vector.

RT-PCR is used for which of the following?

Which of the following individual(s) developed the process of the polymerase chain reaction?

Which of the following would a competent cell be able to do?

In a cloning experiment, you use a vector that contains a lacZ gene as a selectable marker. If the competent cells are grown on X-Gal and IPTG, which colonies would contain chromosomal DNA?

A plasmid that contains separate origins of replication for two different species is called a .

A dideoxy nucleotide is missing an -OH group at the 2' end.

Cloned genes may be mutated outside of a cell by a process called in vitro site-directed mutagensis.

The use of a radioactive probe to screen a DNA library for a gene of interest is called colony hybridization.

What is the origin of restriction endonucleases?

cDNA is made using what as the starting material?

The isolation and copying of a gene, usually in large quantities is called gene cloning.

Which of the following is an example of a palindromic DNA sequence?

What is a DNA library?

R factors typically contain genes for antibiotic resistance.

Transfection is when a bacterial vector is taken up into the host cell.

Southern blotting may be used for which of the following?

Restriction endonucleases are specific in the DNA sequences that they recognize.

The polymerase used in PCR reactions is derived from the thermophile organism Thermus aquaticus.

Which of the following is used to identify a specific DNA sequence in a library?

Overview of Genetics

Mendelian Inheritance

Reproduction and Chromosome Transmission

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria and Bacteriophages

Gene Regulation in Eukaryotes

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular

Biotechnology

Genomics I: Analysis of Dna

Genomics II: Functional Genomics, Proteomics, and

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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