Question 1

Restriction enzymes cut double-stranded DNA

Accepted Answer

A) between genes. 
B) at specific base sequences 
C) between bacterial and viral DNAs. 
D) between purines and pyrimidines. 
E) between promoter and operator DNA sequences. 
A) between genes. 
B) at specific base sequences 
C) between bacterial and viral DNAs. 
D) between purines and pyrimidines. 
E) between promoter and operator DNA sequences.

Question 2

In this step in the identification of the colony that contains a targeted gene,

Accepted Answer

A) bacterial cells are cloned. 
B)  bacterial colonies are transferred. 
C) bacterial cells are ruptured. 
D) DNA is lysed. 
E) cells with the targeted gene are identified. 
A) bacterial cells are cloned. 
B)  bacterial colonies are transferred. 
C) bacterial cells are ruptured. 
D) DNA is lysed. 
E) cells with the targeted gene are identified.

Question 3

RNA can be used as a template for the production of DNA through the action of

Accepted Answer

A) DNA polymerase. 
B) RNA polymerase. 
C) reverse transcriptase. 
D) ligase. 
E) restriction endonuclease. 
A) DNA polymerase. 
B) RNA polymerase. 
C) reverse transcriptase. 
D) ligase. 
E) restriction endonuclease.

Question 4

Because it has no introns, researchers prefer to use __________ when working with eukaryotic genes.

Accepted Answer

A) cDNA 
B) single-stranded DNA 
C) hybridized DNA 
D) bacterial DNA 
E) viral DNA 
A) cDNA 
B) single-stranded DNA 
C) hybridized DNA 
D) bacterial DNA 
E) viral DNA

Question 5

Small circular molecules of "extra" DNA in bacteria

Accepted Answer

A) are plasmids. 
B) are DNA fragments from their main chromosome. 
C) result from the activity of restriction enzymes. 
D) are eventually degraded. 
E) are none of these. 
A) are plasmids. 
B) are DNA fragments from their main chromosome. 
C) result from the activity of restriction enzymes. 
D) are eventually degraded. 
E) are none of these.

Question 6

Bacteria use restriction enzymes to

Accepted Answer

A) integrate viral DNA. 
B) destroy viral DNA. 
C) prevent mutation of their DNA. 
D) copy their genes. 
E) inhibit the expression of some of their genes. 
A) integrate viral DNA. 
B) destroy viral DNA. 
C) prevent mutation of their DNA. 
D) copy their genes. 
E) inhibit the expression of some of their genes.

Question 7

Xenotransplantation refers to

Accepted Answer

A) the substitution of one organ for another. 
B) the transfer of an organ from one individual to another individual of the same species. 
C) transplantation of an organ from an individual of one species to an individual of another species. 
D) transplantation of an organ to augment the function of an otherwise healthy organ. 
E) none of these. 
A) the substitution of one organ for another. 
B) the transfer of an organ from one individual to another individual of the same species. 
C) transplantation of an organ from an individual of one species to an individual of another species. 
D) transplantation of an organ to augment the function of an otherwise healthy organ. 
E) none of these.

Question 8

Genetic engineering has produced which of the following animals?

Accepted Answer

A) goats with heart-healthy milk 
B) low-fat pigs 
C) extra hefty sheep 
D) cows resistant to mad cow disease 
E) all of these 
A) goats with heart-healthy milk 
B) low-fat pigs 
C) extra hefty sheep 
D) cows resistant to mad cow disease 
E) all of these

Question 9

Which statement is true concerning human genetic disorders?

Accepted Answer

A) There are more than 15,000 of them. 
B) Many are rare. 
C) They contribute to many age-related disorders. 
D) They cause 20 to 30 percent of all infant deaths per year. 
E) All of these are true. 
A) There are more than 15,000 of them. 
B) Many are rare. 
C) They contribute to many age-related disorders. 
D) They cause 20 to 30 percent of all infant deaths per year. 
E) All of these are true.

Question 10

Restriction enzymes

Accepted Answer

A) produce staggered cuts in DNA that are often useful in splicing genes. 
B) are very specific in their action. 
C) are natural defense mechanisms in bacteria that destroy foreign DNA introduced by viruses. 
D) are used along with ligases and plasmids to produce DNA libraries. 
E) are all of these. 
A) produce staggered cuts in DNA that are often useful in splicing genes. 
B) are very specific in their action. 
C) are natural defense mechanisms in bacteria that destroy foreign DNA introduced by viruses. 
D) are used along with ligases and plasmids to produce DNA libraries. 
E) are all of these.

Question 11

In automated DNA sequencing, eventually there will be millions of DNA fragments all tagged at

Accepted Answer

A) one end. 
B) both ends. 
C) every nucleotide. 
D) the most numerous nucleotide. 
E) the least numerous nucleotide. 
A) one end. 
B) both ends. 
C) every nucleotide. 
D) the most numerous nucleotide. 
E) the least numerous nucleotide.

Question 12

Sequencing of the human genome was officially completed in 2003 with about __________ percent of the coding regions deciphered with a high degree of accuracy.

Accepted Answer

A) 89 
B) 93 
C) 95 
D) 97 
E) 99 
A) 89 
B) 93 
C) 95 
D) 97 
E) 99

Question 13

DNA chips are used

Accepted Answer

A) to sequence DNA. 
B) in DNA fingerprinting. 
C) to determine which genes are silent or are being expressed. 
D) in PCR. 
E) for all of these. 
A) to sequence DNA. 
B) in DNA fingerprinting. 
C) to determine which genes are silent or are being expressed. 
D) in PCR. 
E) for all of these.

Question 14

The term "biotech barnyards" commonly refers to

Accepted Answer

A) the production of human products by farm animals. 
B) the development of new species of farm animals. 
C) the increase of milk, egg, and wool production. 
D) ultramodern methods of rearing more animals on less feed. 
E) creation of transgenic animals that can produce more of their natural products. 
A) the production of human products by farm animals. 
B) the development of new species of farm animals. 
C) the increase of milk, egg, and wool production. 
D) ultramodern methods of rearing more animals on less feed. 
E) creation of transgenic animals that can produce more of their natural products.

Question 15

Which individual associated with the beginning of the Human Genome Project was concerned with its ethical and social implications?

Accepted Answer

A) Walter Gilbert 
B) James Watson 
C) Francis Crick 
D) Francis Collins 
E) Craig Venter 
A) Walter Gilbert 
B) James Watson 
C) Francis Crick 
D) Francis Collins 
E) Craig Venter

Question 16

The primers in PCR are

Accepted Answer

A) fragments of mRNA. 
B)  designed to base pair with mRNA. 
C) free nucleotides. 
D) radioactive. 
E) none of these. 
A) fragments of mRNA. 
B)  designed to base pair with mRNA. 
C) free nucleotides. 
D) radioactive. 
E) none of these.

Question 17

Although all human beings have unique sets of DNA sequences, about __________ percent of human DNA is the same for all humans.

Accepted Answer

A) 50 
B) 60 
C) 75 
D) 90 
E) 99 
A) 50 
B) 60 
C) 75 
D) 90 
E) 99

Question 18

Which of the following enzymes join the paired sticky ends of DNA fragments?

Accepted Answer

A) reverse transcriptases 
B) restriction enzymes 
C) DNA ligases 
D) DNA polymerases 
E) polynucleotide kinases 
A) reverse transcriptases 
B) restriction enzymes 
C) DNA ligases 
D) DNA polymerases 
E) polynucleotide kinases

Question 19

Which of the following statements is true?

Accepted Answer

A) There is no danger involved in recombinant DNA research in humans. 
B) There is no danger involved in recombinant DNA research in bacteria. 
C) There is no danger in releasing recombinant organisms into the environment. 
D) Stringent safety rules make the use of recombinant DNA research possible. 
E) It is safe to conduct recombinant DNA research in plants. 
A) There is no danger involved in recombinant DNA research in humans. 
B) There is no danger involved in recombinant DNA research in bacteria. 
C) There is no danger in releasing recombinant organisms into the environment. 
D) Stringent safety rules make the use of recombinant DNA research possible. 
E) It is safe to conduct recombinant DNA research in plants.

Question 20

According to the World Health Organization, approximately how many children around the world show a vitamin A deficiency?

Accepted Answer

A) 14 million 
B) 24 million 
C) 140 million 
D) 524 million 
E) 724 million 
A) 14 million 
B) 24 million 
C) 140 million 
D) 524 million 
E) 724 million

Exam 16: Recombinant Dna and Genetic Engineering

Restriction enzymes cut double-stranded DNA

In this step in the identification of the colony that contains a targeted gene,

RNA can be used as a template for the production of DNA through the action of

Because it has no introns, researchers prefer to use __________ when working with eukaryotic genes.

Small circular molecules of "extra" DNA in bacteria

Bacteria use restriction enzymes to

Xenotransplantation refers to

Genetic engineering has produced which of the following animals?

Which statement is true concerning human genetic disorders?

Restriction enzymes

In automated DNA sequencing, eventually there will be millions of DNA fragments all tagged at

Sequencing of the human genome was officially completed in 2003 with about __________ percent of the coding regions deciphered with a high degree of accuracy.

DNA chips are used

The term "biotech barnyards" commonly refers to

Which individual associated with the beginning of the Human Genome Project was concerned with its ethical and social implications?

The primers in PCR are

Although all human beings have unique sets of DNA sequences, about __________ percent of human DNA is the same for all humans.

Which of the following enzymes join the paired sticky ends of DNA fragments?

Which of the following statements is true?

According to the World Health Organization, approximately how many children around the world show a vitamin A deficiency?

Invitation to Biology

Lifes Chemical Basis

Molecules of Life

Cell Structure and Function

A Closer Look at Cell Membranes

Ground Rules of Metabolism

Where It Startsphotosynthesis

Energy-Releasing Pathways

How Cells Reproduce

Meiosis and Sexual Reproduction

Observing Patterns in Inherited Traits

Chromosomes and Human Inheritance

Dna Structure and Function

From Dna to Protein

Controls Over Genes

The Macroevolutionary Puzzle

Emergence of Evolutionary Thought

Organizing Information About Species

The Origin and Evolution of Life

Viruses and Prokaryotes

Protists the Simplest Eukaryotes

Plants

Fungi

Animals: the Invertebrates

Animal Evolution the Chordates

Human Evolution: a Case Study

Plant Tissues

Plant Nutrition and Transport

Plant Reproduction

Plant Growth and Development

Animal Tissues and Organ Systems

Neural Control

Sensory Perception

Endocrine Control

Protection, Support, and Movement

Circulation

Immunity

Respiration

Digestion and Human Nutrition

Maintaining the Internal Environment

Animal Reproductive Systems

Animal Development

Animal Behavior

Population Ecology

Community Structure and Biodiversity

Ecosystems

The Biosphere

Human Impacts on the Biosphere

Filters