Exam 3: Bioenergetics, Enzymes, and Metabolism

An enzyme has a K_M of 20 µM and a V_max of 50 mmoles of product/minute/µg of enzyme.After exposure to an inhibitor and analysis on a Lineweaver - Burk plot the following values are obtained: -1/ K_M = - 0.05 liters/µmole and 1/ V_max = 0.04 (mmoles of product/minute/µg of enzyme)-1.What kind of inhibitor was used in the experiment?

The development of bacterial resistance to antibiotics is an example of what engine of evolution?

How are new compounds typically screened for their effectiveness as antibiotics?

Do the equations below represent coupled reactions? If not,why not? A + B <-> C + D ( $\Delta$ G = +5.4 kcal/mole) D + F <-> G + H ( $\Delta$ G = -4.4 kcal/mole)

Which antibiotic inhibits the enzyme DNA gyrase,which is required for bacterial DNA replication?

You are studying metabolic pathways and discover that two pathways intersect so that the enzyme basinase participates in both of the intersecting pathways,in one case using substrate K and in the other using substrate M.When presented with substrate K in amounts significantly larger than M,basinase converts K to L which leads eventually to the production of the end product R.The activity of the second pathway is depressed simultaneously.In the presence of large amounts of substrate M and lower amounts of substrate K,the second pathway is activated and culminates in the production of that pathway's end product Y.The activity of the first pathway is depressed simultaneously.What are the alternative substrates K and M acting like?

A reaction involving the gain of one or more electrons is a(n)_________ reaction.

Below is a segment of a cell's collection of biochemical pathways.M is a product of one series of these reactions.It is also a regulatory molecule.Look at the pathway below and indicate the position(s)at which M is most likely to act as a feedback inhibitor when its concentration gets too high.