Question 1

One of the first uses of DNA fingerprinting was in _________ medicine.

Accepted Answer

The answer of One of the first uses of DNA...

Question 2

Genomic _____ are collections of isolated genes maintained in a cloning host.

Accepted Answer

The answer of Genomic _____ are collections of isolated genes...

Question 3

When trying to locate a mutation responsible for a disease, exome sequencing would provide an effective shortcut because

Accepted Answer

A) it targets just protein-encoding sequences. 
B) it targets the sequencing or rRNA and tRNA encoding sequences of DNA. 
C) it provides the entire genome sequence. 
D) it targets the sequencing of mitochondrial DNA. 
A) it targets just protein-encoding sequences. 
B) it targets the sequencing or rRNA and tRNA encoding sequences of DNA. 
C) it provides the entire genome sequence. 
D) it targets the sequencing of mitochondrial DNA.

Question 4

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Accepted Answer

The answer of The two common vectors used to transfer...

Question 5

Which of the following provides the specificity of the CRISPR/Cas gene editing system?

Accepted Answer

A) the amino acids at the amino terminus of the Cas nuclease 
B) the guide RNA (crRN
A)  carried by the Cas nuclease 
C) the amino acids at the carboxyl terminus of the Cas nuclease 
D) an antibody-like region of the Cas nuclease 
A) the amino acids at the amino terminus of the Cas nuclease 
B) the guide RNA (crRN
A)  carried by the Cas nuclease 
C) the amino acids at the carboxyl terminus of the Cas nuclease 
D) an antibody-like region of the Cas nuclease

Question 6

The fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A) DNA. 
B) RNA. 
C) proteins. 
D) recombinant DNA. 
E) specific genetic marker sequence on genes. 
A) DNA. 
B) RNA. 
C) proteins. 
D) recombinant DNA. 
E) specific genetic marker sequence on genes.

Question 7

Although originally discovered in bacteria as a defense against bacteriophage infection, the CRISPR/Cas gene editing system can be used to edit the genome of any cell, whether microbial, plant, fungal, or animal.

Accepted Answer

A) True 
 B)False

Question 8

Two different nucleic acids can _____ by uniting at their complementary sites.

Accepted Answer

The answer of Two different nucleic acids can _____ by...

Question 9

The primers in PCR are

Accepted Answer

A) synthetic DNA oligonucleotides. 
B) bacterial enzymes. 
C) short RNA strands. 
D) DNA polymerases. 
E) reverse transcriptases. 
A) synthetic DNA oligonucleotides. 
B) bacterial enzymes. 
C) short RNA strands. 
D) DNA polymerases. 
E) reverse transcriptases.

Question 10

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 11

When DNA is heated, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 12

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Accepted Answer

A) PCR 
B) genetic engineering 
C) antisense technology 
D) microarray analysis 
E) DNA sequencing 
A) PCR 
B) genetic engineering 
C) antisense technology 
D) microarray analysis 
E) DNA sequencing

Question 13

Transgenic animals

Accepted Answer

A) can be engineered to become factories for manufacturing proteins. 
B) are often obtained from germ line engineering. 
C) will pass the genes on to their offspring. 
D) commonly include mice. 
E) All of these choices are correct. 
A) can be engineered to become factories for manufacturing proteins. 
B) are often obtained from germ line engineering. 
C) will pass the genes on to their offspring. 
D) commonly include mice. 
E) All of these choices are correct.

Question 14

Transformation and transfection are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 15

Transgenic animals are _____ modified organisms.

Accepted Answer

The answer of Transgenic animals are _____ modified organisms....

Question 16

The enzyme___ adds nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated.
A.  DNA polymerase III, 3’→	5’
B.  DNA polymerase I, 3’→	5’
C.  DNA polymerase III, 5’→	3’		
D.  DNA polymerase I, 5’→	3’

Accepted Answer

The answer of The enzyme___ adds nucleotides of DNA in...

Question 17

The commercial product Frostban contains a genetically engineered strain of

Accepted Answer

A) Escherichia coli. 
B) Saccharomyces cerevisiae. 
C) Thermus aquaticus. 
D) Pseudomonas syringae. 
E) Pseudomonas fluorescens. 
A) Escherichia coli. 
B) Saccharomyces cerevisiae. 
C) Thermus aquaticus. 
D) Pseudomonas syringae. 
E) Pseudomonas fluorescens.

Question 18

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 19

The various techniques by which scientists manipulate DNA in the lab are called

Accepted Answer

A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA. 
D) gel electrophoresis. 
E) gene probes. 
A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA. 
D) gel electrophoresis. 
E) gene probes.

Question 20

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

Accepted Answer

A) True 
 B)False

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

One of the first uses of DNA fingerprinting was in _________ medicine.

Genomic _____ are collections of isolated genes maintained in a cloning host.

When trying to locate a mutation responsible for a disease, exome sequencing would provide an effective shortcut because

The two common vectors used to transfer a piece of DNA into a cloning host are _____ and _____.

Which of the following provides the specificity of the CRISPR/Cas gene editing system?

The fluorescent in situ hybridization (FISH) probes are applied to intact cells and observed microscopically for the presence and location of

Although originally discovered in bacteria as a defense against bacteriophage infection, the CRISPR/Cas gene editing system can be used to edit the genome of any cell, whether microbial, plant, fungal, or animal.

Two different nucleic acids can _____ by uniting at their complementary sites.

The primers in PCR are

Restriction endonucleases are obtained from various species of bacteria.

When DNA is heated, the two strands will separate.

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Transgenic animals

Transformation and transfection are methods used to introduce DNA into host cells.

Transgenic animals are _____ modified organisms.

The enzyme___ adds nucleotides of DNA in the ___direction when DNA is being semi-conservatively replicated. A. DNA polymerase III, 3’→ 5’ B. DNA polymerase I, 3’→ 5’ C. DNA polymerase III, 5’→ 3’ D. DNA polymerase I, 5’→ 3’

The commercial product Frostban contains a genetically engineered strain of

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

The various techniques by which scientists manipulate DNA in the lab are called

After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

Filters

The two common vectors used to transfer a piece of DNA into a cloning host are _ and _.

The enzyme_ adds nucleotides of DNA in the _direction when DNA is being semi-conservatively replicated. A. DNA polymerase III, 3’→ 5’ B. DNA polymerase I, 3’→ 5’ C. DNA polymerase III, 5’→ 3’ D. DNA polymerase I, 5’→ 3’