Question 1

All of the following are characteristics that make plasmids good cloning vectors, except

Accepted Answer

A) they are small. 
B) they are well characterized. 
C) they can be transferred into appropriate host cells. 
D) they can carry genetic markers, such as antibiotic resistance genes. 
E) they can accept a relatively large amount of foreign DNA. 
A) they are small. 
B) they are well characterized. 
C) they can be transferred into appropriate host cells. 
D) they can carry genetic markers, such as antibiotic resistance genes. 
E) they can accept a relatively large amount of foreign DNA.

Question 2

Which of the following is often used in forensic science to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci?

Accepted Answer

A) cloning 
B) gene therapy 
C) antisense therapeutic 
D) DNA fingerprinting 
A) cloning 
B) gene therapy 
C) antisense therapeutic 
D) DNA fingerprinting

Question 3

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Accepted Answer

The answer of In recombinant DNA technique, an enzyme called...

Question 4

Compare and contrast recombinant DNA and gene therapy.

Accepted Answer

Recombinant DNA and gene therapy are bot

Question 5

When DNA is heated to just below boiling (90°C-95°C),

Accepted Answer

A) the sugar phosphate backbone of the DNA strands breaks. 
B) the sugar, phosphate, and base come apart in each nucleotide. 
C) the helix unwinds but hydrogen bonds between the bases remain intact. 
D) the two DNA strands separate completely. 
E) nothing happens until the boiling point is reached (100o
C) . 
A) the sugar phosphate backbone of the DNA strands breaks. 
B) the sugar, phosphate, and base come apart in each nucleotide. 
C) the helix unwinds but hydrogen bonds between the bases remain intact. 
D) the two DNA strands separate completely. 
E) nothing happens until the boiling point is reached (100o
C) .

Question 6

All of the following are correct about DNA fingerprinting, except

Accepted Answer

A) it displays the unique pattern of an individual's DNA fragments. 
B) it can be used to analyze evidence when only minute amounts of DNA are found. 
C) it cannot be used on specimens older than five years. 
D) it is routine to use it as trial evidence in the U.S. 
E) it can be used on hair and saliva. 
A) it displays the unique pattern of an individual's DNA fragments. 
B) it can be used to analyze evidence when only minute amounts of DNA are found. 
C) it cannot be used on specimens older than five years. 
D) it is routine to use it as trial evidence in the U.S. 
E) it can be used on hair and saliva.

Question 7

EcoRI and HindIII are

Accepted Answer

A) palindromes. 
B) reverse transcriptases. 
C) restriction endonucleases. 
D) ligases. 
E) DNA polymerases. 
A) palindromes. 
B) reverse transcriptases. 
C) restriction endonucleases. 
D) ligases. 
E) DNA polymerases.

Question 8

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

Accepted Answer

1. Start by growing E. coli cells in a l

Question 9

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Accepted Answer

A) True 
 B)False

Question 10

Some genes in bacteria are repressed by antisense RNA that binds to them.

Accepted Answer

A) True 
 B)False

Question 11

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

Accepted Answer

A) RNA promoters 
B) fluorescent nucleotides 
C) primers 
D) thermophilic polymerases 
E) endonucleases 
A) RNA promoters 
B) fluorescent nucleotides 
C) primers 
D) thermophilic polymerases 
E) endonucleases

Question 12

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Accepted Answer

The answer of The _____ blot method analyzes DNA, while...

Question 13

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Accepted Answer

A) DNA fingerprinting 
B) microarray analysis 
C) gene mapping 
D) Western blot 
E) antisense therapy 
A) DNA fingerprinting 
B) microarray analysis 
C) gene mapping 
D) Western blot 
E) antisense therapy

Question 14

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

Accepted Answer

A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA. 
D) gel electrophoresis. 
E) gene probes. 
A) genetic engineering. 
B) biotechnology. 
C) recombinant DNA. 
D) gel electrophoresis. 
E) gene probes.

Question 15

The size of DNA is often given in the number of _____ that it contains.

Accepted Answer

base pairs

Question 16

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Accepted Answer

A) Human growth hormone 
B) Factor VIII 
C) insulin 
D) tissue plasminogen activator 
E) All of these choices are correct. 
A) Human growth hormone 
B) Factor VIII 
C) insulin 
D) tissue plasminogen activator 
E) All of these choices are correct.

Question 17

Which step involves transformation?

Accepted Answer

A) Target DNA is removed from cells and isolated. 
B) The cloning host takes up a plasmid. 
C) Target DNA and plasmid are treated with the same restriction endonuclease. 
D) The desired protein is produced by the cloning host. 
E) The gene is amplified by multiplication of the cloning host. 
A) Target DNA is removed from cells and isolated. 
B) The cloning host takes up a plasmid. 
C) Target DNA and plasmid are treated with the same restriction endonuclease. 
D) The desired protein is produced by the cloning host. 
E) The gene is amplified by multiplication of the cloning host.

Question 18

To be of value, DNA sequences targeted for analysis for DNA profiling need to be located in coding regions of the genome.

Accepted Answer

A) True 
 B)False

Question 19

Which PCR step synthesizes complementary DNA strands?

Accepted Answer

A) Add DNA polymerase and nucleotides at 72°C. 
B) Cool DNA to between 50°C and 65°C. 
C) Add primers. 
D) Heat target DNA to 94°C. 
E) Repeat the cycle of heating and cooling. 
A) Add DNA polymerase and nucleotides at 72°C. 
B) Cool DNA to between 50°C and 65°C. 
C) Add primers. 
D) Heat target DNA to 94°C. 
E) Repeat the cycle of heating and cooling.

Question 20

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

Accepted Answer

A DNA fingerprint is a unique pattern of

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

All of the following are characteristics that make plasmids good cloning vectors, except

Which of the following is often used in forensic science to distinguish one sequence of DNA from another by comparing the sequence of the strands at specific loci?

In recombinant DNA technique, an enzyme called _____ is needed to seal the sticky ends of genes into plasmids or chromosomes.

Compare and contrast recombinant DNA and gene therapy.

When DNA is heated to just below boiling (90°C-95°C),

All of the following are correct about DNA fingerprinting, except

EcoRI and HindIII are

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E.coli cells growing in a lab culture.

VNTRs and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting.

Some genes in bacteria are repressed by antisense RNA that binds to them.

Both DNA sequencing and polymerase chain reactions require special __________ to initiate synthesis of a new DNA molecule.

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Which of the following methods determines which genes are actively transcribed in a cell under a variety of conditions?

Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are

The size of DNA is often given in the number of _____ that it contains.

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Which step involves transformation?

To be of value, DNA sequences targeted for analysis for DNA profiling need to be located in coding regions of the genome.

Which PCR step synthesizes complementary DNA strands?

Describe what is meant by a DNA fingerprint and how it is used in forensic science.

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

Filters

The _ blot method analyzes DNA, while the _ method analyzes RNA.