Question 1

Two-dimensional electrophoresis is a combination of what two techniques?

Accepted Answer

A)  ion-exchange chromatography and affinity chromatography 
B)  ion-exchange chromatography and SDS-PAGE 
C)  SDS-PAGE and affinity chromatography 
D)  isoelectric focusing and SDS-PAGE 
E)  isoelectric focusing and ion-exchange chromatography 
A)  ion-exchange chromatography and affinity chromatography 
B)  ion-exchange chromatography and SDS-PAGE 
C)  SDS-PAGE and affinity chromatography 
D)  isoelectric focusing and SDS-PAGE 
E)  isoelectric focusing and ion-exchange chromatography

Question 2

Briefly describe how an ELISA works.

Accepted Answer

In ELISA,the antigen of interest is comp

Question 3

Another name for an antigenic determinant is _____.

Accepted Answer

A)  epitope 
B)  epimer 
C)  epinephrine 
D)  epidemic 
E)  None of the answers is correct. 
A)  epitope 
B)  epimer 
C)  epinephrine 
D)  epidemic 
E)  None of the answers is correct.

Question 4

What types of molecules can serve as antigens?

Accepted Answer

A)  proteins 
B)  polysaccharides 
C)  metal ions 
D)  All of the answers are correct. 
E)  proteins and polysaccharides 
A)  proteins 
B)  polysaccharides 
C)  metal ions 
D)  All of the answers are correct. 
E)  proteins and polysaccharides

Question 5

In the Edman procedure for peptide sequence,phenyl isothiocyanate is used to selectively remove the __________________ residue as a PTH-derivative.

Accepted Answer

The answer of In the Edman procedure for peptide sequence,phenyl...

Question 6

Which of the following techniques can be used to determine mass to charge ratio of a molecule?

Accepted Answer

A)  Edman degradation 
B)  affinity chromatography 
C)  diagonal electrophoresis 
D)  MALDI-TOF 
E)  SDS-PAGE 
A)  Edman degradation 
B)  affinity chromatography 
C)  diagonal electrophoresis 
D)  MALDI-TOF 
E)  SDS-PAGE

Question 7

__________________ gels are often used as the media for electrophoretic techniques such as SDS-PAGE and isoelectric focusing.

Accepted Answer

The answer of __________________ gels are often used as the...

Question 8

_____ is the term to describe an original amino acid sequence for an uncleaved protein.

Accepted Answer

A)  Apoprotein 
B)  Holoprotein 
C)  Nascent 
D)  Protomer 
E)  None of the answers is correct. 
A)  Apoprotein 
B)  Holoprotein 
C)  Nascent 
D)  Protomer 
E)  None of the answers is correct.

Question 9

An ELISA can be used for

Accepted Answer

A)  quantitative analysis. 
B)  size analysis. 
C)  protein sequencing 
D)  All of the answers are correct. 
E)  None of the answers is correct. 
A)  quantitative analysis. 
B)  size analysis. 
C)  protein sequencing 
D)  All of the answers are correct. 
E)  None of the answers is correct.

Question 10

Why is an assay necessary for protein purification studies?

Accepted Answer

An assay allows researchers to accuratel

Exam 3: Exploring Proteins and Proteomes

Two-dimensional electrophoresis is a combination of what two techniques?

Briefly describe how an ELISA works.

Another name for an antigenic determinant is _____.

What types of molecules can serve as antigens?

In the Edman procedure for peptide sequence,phenyl isothiocyanate is used to selectively remove the __________________ residue as a PTH-derivative.

Which of the following techniques can be used to determine mass to charge ratio of a molecule?

__________________ gels are often used as the media for electrophoretic techniques such as SDS-PAGE and isoelectric focusing.

_____ is the term to describe an original amino acid sequence for an uncleaved protein.

An ELISA can be used for

Why is an assay necessary for protein purification studies?

Biochemistry: An Evolving Science

Protein Composition and Structure

DNA, RNA, and the Flow of Genetic Information

Exploring Genes and Genomes

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

Oxidative Phosphorylation

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, repair, and Recombination

RNA Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

Filters