Exam 3: Exploring Proteins and Proteomes

The unit for sedimentation coefficients is the _____.

What technique would be used to separate proteins with different sedimentation coefficients?

The ratio of enzyme activity relative to total protein is called _____.

How can the amino acid sequences be used to design a DNA probe?

The use of synthetic peptides includes

_____ can be added prior to gel electrophoresis to fully denature proteins.

What technique can be used to determine the mass of a protein without knowing the identity of the molecule?

How can a protein's isoelectric point be used in protein purification (i.e.,isoelectric focusing)?

How can recombinant DNA technology aid in protein purification?

Long proteins are often treated with the enzyme ______________,which cleaves the protein into smaller,easily analyzed peptides.

Polypeptides can be fragmented into smaller peptides by cleavage with trypsin,which hydrolyzes the peptide bond at the C-terminal side of __________________ residues.

What is one advantage of using the recombinant DNA methods to determine protein sequences?

A technique used to identify proteins after gel electrophoresis,which employs antibodies in the detection process,is known as

Why are monoclonal antibodies more useful than polyclonal antibodies?

How are monoclonal antibodies made?

Cyanogen bromide cleaves the peptide bond at

After a protein is purified,what is the next step in determining the structure of a protein by x-ray crystallography?

Dithiothreitol or β mercaptoethanol are commonly used to reduce _______________ bonds.

What is the purpose of determining the specific activity,yield,and purification level of a protein purification protocol?

Molecular exclusion or gel-filtration chromatography separates molecules on the basis of __________________.