Question 1

A multiple clonic site is also known as:

Accepted Answer

A)  a polylinker 
B)  an origin of replication 
C)  a restriction enzyme site 
D)  a selectable marker 
A)  a polylinker 
B)  an origin of replication 
C)  a restriction enzyme site 
D)  a selectable marker

Question 2

When fluorescent markers are used for DNA sequencing:

Accepted Answer

A)  the DNA separations are faster 
B)  four different reaction mixtures must be used 
C)  computer analysis can help in the detection of sequencing analysis at a faster rate 
D)  none of these 
A)  the DNA separations are faster 
B)  four different reaction mixtures must be used 
C)  computer analysis can help in the detection of sequencing analysis at a faster rate 
D)  none of these

Question 3

Recombinant DNA molecules contain covalently linked segments derived from two or more sources.

Accepted Answer

A) True 
 B)False

Question 4

The use of the Bt gene is prohibited in corn plants.

Accepted Answer

A) True 
 B)False

Question 5

How is human insulin produced by genetically engineered bacteria?

Accepted Answer

A)  DNA that codes for each of the two polypeptide chains is introduced into two different populations of bacteria 
B)  DNA that codes for the insulin precursor is introduced into bacterial plasmids 
C)  DNA that codes for both the polypeptide chains is introduced into a single population of bacteria 
D)  This procedure cannot be done. 
A)  DNA that codes for each of the two polypeptide chains is introduced into two different populations of bacteria 
B)  DNA that codes for the insulin precursor is introduced into bacterial plasmids 
C)  DNA that codes for both the polypeptide chains is introduced into a single population of bacteria 
D)  This procedure cannot be done.

Question 6

Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs.The b-gal gene codes for the enzyme b-galactosidase,the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpR gene confers ampicillin resistance.
  Refer to Exhibit 13D.Neglecting any discussion of whether it's a good or bad choice,I attempt to insert a gene fragment into the HindIII site and transform bacteria with the plasmid.How can I tell which transformants have the insert?

Accepted Answer

A)  The bacteria will not be able to grow in the presence of ampicillin, and they will be blue. 
B)  The bacteria will not be able to grow in the presence of ampicillin, and they will be white. 
C)  The bacteria will be able to grow in the presence of ampicillin, and they will be blue. 
D)  The bacteria will be able to grow in the presence of ampicillin, and they will be white. 
A)  The bacteria will not be able to grow in the presence of ampicillin, and they will be blue. 
B)  The bacteria will not be able to grow in the presence of ampicillin, and they will be white. 
C)  The bacteria will be able to grow in the presence of ampicillin, and they will be blue. 
D)  The bacteria will be able to grow in the presence of ampicillin, and they will be white.

Question 7

The "natural" function of restriction endonucleases is to

Accepted Answer

A)  protect bacterial cells from invasion by viruses (bacteriophages). 
B)  help bacteriophages infect cells. 
C)  regulate gene expression from specific promoters. 
D)  remove chromatin from histones. 
A)  protect bacterial cells from invasion by viruses (bacteriophages). 
B)  help bacteriophages infect cells. 
C)  regulate gene expression from specific promoters. 
D)  remove chromatin from histones.

Question 8

If mRNA is converted to DNA,digested with restriction enzymes,and cloned into suitable vectors,the resultant library is called a:

Accepted Answer

A)  DNA library 
B)  cDNA library 
C)  mRNA library 
D)  none of these 
A)  DNA library 
B)  cDNA library 
C)  mRNA library 
D)  none of these

Question 9

Which of the following proteins are produced by recombinant DNA technology but have had their purposes subverted for improving athletic perforrmance?

Accepted Answer

A)  Erythropoietin 
B)  Human Growth Hormone 
C)  Insulin 
D)  Enterokinase 
E)  Erythropoietin and Human Growth Hormone
F) All of these 
A)  Erythropoietin 
B)  Human Growth Hormone 
C)  Insulin 
D)  Enterokinase 
E)  Erythropoietin and Human Growth Hormone
F) All of these

Question 10

The polymerase chain reaction requires

Accepted Answer

A)  primers complementary to the ends of the sequence to be amplified 
B)  careful temperature control 
C)  both of these 
D)  neither of these 
A)  primers complementary to the ends of the sequence to be amplified 
B)  careful temperature control 
C)  both of these 
D)  neither of these

Question 11

Which of the following are methods used to determine where the DNA bands are located on an electrophoresis gel?

Accepted Answer

A)  Radioactivity 
B)  Fluorescence 
C)  Dyes which bind to DNA 
D)  Luminescence 
E)  All of these can visualize the DNA 
A)  Radioactivity 
B)  Fluorescence 
C)  Dyes which bind to DNA 
D)  Luminescence 
E)  All of these can visualize the DNA

Question 12

Fluorescence works because the fluorescent molecule

Accepted Answer

A)  absorbs light at one wavelength and emits light at a longer wavelength. 
B)  absorbs light at one wavelength and emits light at a shorter wavelength. 
C)  absorbs light at one wavelength and emits light at the same wavelength. 
D)  absorbs light at many wavelengths and emits light at many wavelengths. 
A)  absorbs light at one wavelength and emits light at a longer wavelength. 
B)  absorbs light at one wavelength and emits light at a shorter wavelength. 
C)  absorbs light at one wavelength and emits light at the same wavelength. 
D)  absorbs light at many wavelengths and emits light at many wavelengths.

Question 13

The "c" in cDNA stands for this word:

Accepted Answer

A)  Complete. 
B)  Circular. 
C)  Complementary. 
D)  Chromosomal. 
E)  Confusing. 
A)  Complete. 
B)  Circular. 
C)  Complementary. 
D)  Chromosomal. 
E)  Confusing.

Question 14

Cells that contain a "blue/white screening" plasmid that has an added gene are recognized by the method:

Accepted Answer

A)  Ability to grow on ampicillin. 
B)  Inability to grow on ampicillin. 
C)  The colonies have a blue color. 
D)  The colonies lack a blue color. 
E)  More than one of these choices indicates that the plasmid contains recombined DNA. 
A)  Ability to grow on ampicillin. 
B)  Inability to grow on ampicillin. 
C)  The colonies have a blue color. 
D)  The colonies lack a blue color. 
E)  More than one of these choices indicates that the plasmid contains recombined DNA.

Question 15

Which of the following is not required in order for a plasmid to be used in genetic recombination?

Accepted Answer

A)  An "ori" site. 
B)  A gene to allow for easy screening an isolation of cells which contain the plasmid. 
C)  A poly-cloning site or multi-cloning site for gene insertion. 
D)  At least one site for a restriction enzyme to cut. 
E)  All of these are essential in a plasmid for DNA recombination. 
A)  An "ori" site. 
B)  A gene to allow for easy screening an isolation of cells which contain the plasmid. 
C)  A poly-cloning site or multi-cloning site for gene insertion. 
D)  At least one site for a restriction enzyme to cut. 
E)  All of these are essential in a plasmid for DNA recombination.

Question 16

Which of the following plants have been genetically modified?

Accepted Answer

A)  Soy beans 
B)  Corn. 
C)  Cotton 
D)  Tomatoes 
E)  All of these plants have been genetically modified. 
A)  Soy beans 
B)  Corn. 
C)  Cotton 
D)  Tomatoes 
E)  All of these plants have been genetically modified.

Question 17

Genetic recombination occurs in nature.

Accepted Answer

A) True 
 B)False

Question 18

In gene therapy

Accepted Answer

A)  restriction enzymes are introduced into cells to cleave specific genes. 
B)  new origins of replication are added to cells to promote cell division. 
C)  cells of specific tissues are altered in a way that alleviates the effects of a disease. 
D)  traits are altered so that descendents of those treated will inherit those new traits. 
A)  restriction enzymes are introduced into cells to cleave specific genes. 
B)  new origins of replication are added to cells to promote cell division. 
C)  cells of specific tissues are altered in a way that alleviates the effects of a disease. 
D)  traits are altered so that descendents of those treated will inherit those new traits.

Question 19

A palindrome is

Accepted Answer

A)  a DNA sequence that contains only one kind of base 
B)  a DNA sequence that contains only two kinds of bases 
C)  a sequence that reads the same from left to right or from right to left 
D)  none of the above 
A)  a DNA sequence that contains only one kind of base 
B)  a DNA sequence that contains only two kinds of bases 
C)  a sequence that reads the same from left to right or from right to left 
D)  none of the above

Question 20

A proteome is

Accepted Answer

A)  a protein-based vector. 
B)  a three-dimensional protein structure. 
C)  a collection of all the proteins produced in a given cell or tissue. 
D)  an improperly digested protein responsible for certain diseases such as "mad cow" disease. 
A)  a protein-based vector. 
B)  a three-dimensional protein structure. 
C)  a collection of all the proteins produced in a given cell or tissue. 
D)  an improperly digested protein responsible for certain diseases such as "mad cow" disease.

Exam 13: Nucleic Acid Biotechnology Techniques

A multiple clonic site is also known as:

When fluorescent markers are used for DNA sequencing:

Recombinant DNA molecules contain covalently linked segments derived from two or more sources.

The use of the Bt gene is prohibited in corn plants.

How is human insulin produced by genetically engineered bacteria?

The "natural" function of restriction endonucleases is to

If mRNA is converted to DNA,digested with restriction enzymes,and cloned into suitable vectors,the resultant library is called a:

Which of the following proteins are produced by recombinant DNA technology but have had their purposes subverted for improving athletic perforrmance?

The polymerase chain reaction requires

Which of the following are methods used to determine where the DNA bands are located on an electrophoresis gel?

Fluorescence works because the fluorescent molecule

The "c" in cDNA stands for this word:

Cells that contain a "blue/white screening" plasmid that has an added gene are recognized by the method:

Which of the following is not required in order for a plasmid to be used in genetic recombination?

Which of the following plants have been genetically modified?

Genetic recombination occurs in nature.

In gene therapy

A palindrome is

A proteome is

Biochemistry and the Organization of Cells

Water: The Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three-Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins: Enzymes

The Behavior of Proteins: Enzymes, Mechanisms, and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids: How Structure Conveys Information

Biosynthesis of Nucleic Acids: Replication

Transcription of the Genetic Code: Biosynthesis of Rna

Protein Synthesis: Translation of the Genetic Message

Viruses, Cancer, and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism: Cellular Signaling

Filters