Question 1

Which type of media is designed to support the growth of one group of organisms,but not another,by containing antimicrobials,dyes,or alcohol?

Accepted Answer

A) Selective 
B) Nutritive 
C) Differential 
D) Definitive 
A) Selective 
B) Nutritive 
C) Differential 
D) Definitive

Question 2

Which type of media is designed to distinguish microorganisms on the basis of certain growth characteristics?

Accepted Answer

A) Selective 
B) Nutritive 
C) Differential 
D) Definitive 
A) Selective 
B) Nutritive 
C) Differential 
D) Definitive

Question 3

Which specimen is quantitatively plated by using a calibrated loop?

Accepted Answer

A) Stool 
B) Sputum 
C) Joint fluid 
D) Urine 
A) Stool 
B) Sputum 
C) Joint fluid 
D) Urine

Question 4

The microbiology laboratory receives a genital culture from a 28-year-old female.The specimen direct Gram stain reveals,gram-positive cocci in clusters and gram-negative cocci in chains.The microbiologist inoculates a sheep blood agar plate,a chocolate agar plate,a modified thayer martin plate and a LIM Broth (selective enrichment for group B streptococci).The second shift microbiologist identifies the discrepancy and must take corrective action.The discrepancy indicates:

Accepted Answer

A) there is no discrepancy noted. 
B) the preliminary Gram stain was erroneously reported. 
C) the microbiologist should cancel this order and reorder a genital screen for group B streptococci. 
D) the microbiologist should report out the results from the cultures when growth is evident. 
A) there is no discrepancy noted. 
B) the preliminary Gram stain was erroneously reported. 
C) the microbiologist should cancel this order and reorder a genital screen for group B streptococci. 
D) the microbiologist should report out the results from the cultures when growth is evident.

Question 5

A definitive identification of a pathogen typically includes:

Accepted Answer

A) a description of the colonial and microscopic morphology. 
B) a description of the colonial and biochemical reactions. 
C) a confirmatory set of reactions that correlates to the preliminary characteristics. 
D) a confirmatory set of reactions and antibiogram for all organisms. 
A) a description of the colonial and microscopic morphology. 
B) a description of the colonial and biochemical reactions. 
C) a confirmatory set of reactions that correlates to the preliminary characteristics. 
D) a confirmatory set of reactions and antibiogram for all organisms.

Question 6

Ambient air,which contains 21% oxygen (O₂)and a small amount (0.03%)of carbon dioxide (CO₂),is the environmental condition that best suits which type of organism?

Accepted Answer

A) Aerobes 
B) Anaerobes 
C) Capnophiles 
D) Microaerophiles 
A) Aerobes 
B) Anaerobes 
C) Capnophiles 
D) Microaerophiles

Question 7

The appropriate storage for a cerebral spinal fluid specimen is ____°C.

Accepted Answer

A) 4 
B) 22 
C) 37 
D) -70 
A) 4 
B) 22 
C) 37 
D) -70

Question 8

The microbiology laboratory receives a nasopharyngeal specimen.The technologist fails to report the direct Gram stain and inoculates the sample onto routine culture media including blood agar,chocolate agar,and MacConkey agar.The discrepancy is:

Accepted Answer

A) there is no discrepancy. 
B) the microbiologist failed to report the direct Gram stain. 
C) the microbiologist failed to report the direct Gram stain and inoculated unnecessary media. 
D) the microbiologist inoculated unnecessary media. 
A) there is no discrepancy. 
B) the microbiologist failed to report the direct Gram stain. 
C) the microbiologist failed to report the direct Gram stain and inoculated unnecessary media. 
D) the microbiologist inoculated unnecessary media.

Exam 5: Specimen Management

Which type of media is designed to support the growth of one group of organisms,but not another,by containing antimicrobials,dyes,or alcohol?

Which type of media is designed to distinguish microorganisms on the basis of certain growth characteristics?

Which specimen is quantitatively plated by using a calibrated loop?

A definitive identification of a pathogen typically includes:

Ambient air,which contains 21% oxygen (O₂)and a small amount (0.03%)of carbon dioxide (CO₂),is the environmental condition that best suits which type of organism?

The appropriate storage for a cerebral spinal fluid specimen is ____°C.

The microbiology laboratory receives a nasopharyngeal specimen.The technologist fails to report the direct Gram stain and inoculates the sample onto routine culture media including blood agar,chocolate agar,and MacConkey agar.The discrepancy is:

Microbial Taxonomy

Bacterial Genetics, metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Overview of Immunochemical Methods Used for Organism Detection

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

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Francisella

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Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Overview of the Methods and Strategies in Parasitology

Intestinal Protozoa

Blood and Tissue Protozoa

Protozoa From Other Body Sites

Intestinal Nematodes

Tissue Nematodes

Blood Nematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Zygomycetes, Dermatophytes, and Opportunistic and Systemic Mycoses

Dematiaceious Molds

Opportunistic Atypical Fungus: Pjirovecii

The Yeasts

Anti-fungal Susceptibility Testing, therapy, and Prevention

Overview of the Methods and Strategies in Virology

Viruses in Human Disease

Antiviral Therapy Susceptibility Testing and Prevention

Bloodstream Infections

Infections of the Lower Respiratory Tract

Upper Respiratory Tract Infections and Other Infections of the Oral Cavity and Neck

Meningitis and Other Infections of the Central Nervous Systems

Infections of the Eyes ears and Sinuses

Infections of the Urinary Tract

Genital Tract Infections

Gastrointestinal Tract Infections