Question 1

Match the assay with its function.
-Quantifies the amount of viruses infected in cells.

Accepted Answer

A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing 
A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing D

Question 2

Match the virus with the appropriate antiviral agent.
-Ribavirin

Accepted Answer

A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV 
A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV D

Question 3

The only virus that has been globally eradicated is:

Accepted Answer

A) chicken pox. 
B) cowpox. 
C) smallpox. 
D) swinepox. 
A) chicken pox. 
B) cowpox. 
C) smallpox. 
D) swinepox. C

Question 4

The recombinant virus assay (RVA)uses which type of amplification methodology?

Accepted Answer

A) Transcription-mediated amplification (TM
A)  
B) Nucleic acid sequence-based amplification (NASB
A)  
C) Strand displacement amplification (SD
A)  
D) Reverse transcription polymerase chain reaction (RT-PCR) 
A) Transcription-mediated amplification (TM
A)  
B) Nucleic acid sequence-based amplification (NASB
A)  
C) Strand displacement amplification (SD
A)  
D) Reverse transcription polymerase chain reaction (RT-PCR)

Question 5

Match the virus with the appropriate antiviral agent.
-Amantadine

Accepted Answer

A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV 
A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV

Question 6

Match the assay with its function.
-Inhibits viral DNA synthesis.

Accepted Answer

A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing 
A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing

Question 7

Match the assay with its function.
-Quantifies sequence variation.

Accepted Answer

A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing 
A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing

Question 8

Match the assay with its function.
-Inhibits viral plaque formation.

Accepted Answer

A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing 
A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing

Question 9

Match the virus with the appropriate antiviral agent.
-Emtricitabine

Accepted Answer

A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV 
A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV

Question 10

Match the virus with the appropriate antiviral agent.
-Acyclovir

Accepted Answer

A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV 
A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV

Question 11

If a physician prescribes Tamiflu to a patient,the patient is most likely infected with which virus?

Accepted Answer

A) HIV 
B) Herpes simplex virus (HSV) 
C) Cytomegalovirus (CMV) 
D) Influenza 
A) HIV 
B) Herpes simplex virus (HSV) 
C) Cytomegalovirus (CMV) 
D) Influenza

Question 12

When performing antiviral susceptibility testing,a standard inoculum must be used.If the inoculum quantity is too large,then the isolate will appear:

Accepted Answer

A) susceptible. 
B) intermediate. 
C) resistant. 
D) indeterminate. 
A) susceptible. 
B) intermediate. 
C) resistant. 
D) indeterminate.

Question 13

Match the assay with its function.
-Viable cells take up neutral red dye.

Accepted Answer

A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing 
A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing

Question 14

Point mutations in the hemagglutination (HA)or neuraminidase (NA)genes during viral ribonucleoprotein (RNP)replication give rise to new strains.This mechanism is known as the antigenic:

Accepted Answer

A) drift. 
B) shift. 
C) re-assortment. 
D) modification. 
A) drift. 
B) shift. 
C) re-assortment. 
D) modification.

Question 15

Match the assay with its function.
-Detects resistance genes.

Accepted Answer

A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing 
A) Phenotypic assay 
B) Plaque reduction assay 
C) Du assay 
D) Flow cytometry 
E) Genotypic susceptibility assay
F)Pyrosequencing

Question 16

Which type of assay uses a variety of endpoint measurements to determine whether the virus is inhibited by the antiviral drug or is demonstrating drug resistance?

Accepted Answer

A) Phenotypic 
B) Genotypic 
C) NA inhibition assay 
D) Enzyme immunoassay 
A) Phenotypic 
B) Genotypic 
C) NA inhibition assay 
D) Enzyme immunoassay

Question 17

Match the virus with the appropriate antiviral agent.
-Lamivudine

Accepted Answer

A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV 
A) HSV 
B) Influenza A 
C) Hepatitis B virus (HBV) 
D) Respiratory syncytial virus (RSV) 
E) HIV

Question 18

At the end of the 2008-2009 influenza season,100% of influenza A H3N2 and the novel 2009 influenza A H1N1 were resistant to:

Accepted Answer

A) adamantane. 
B) oseltamivir. 
C) Tamiflu. 
D) Relenza. 
A) adamantane. 
B) oseltamivir. 
C) Tamiflu. 
D) Relenza.

Exam 65: Antiviral Therapy Susceptibility Testing and Prevention

Match the assay with its function. -Quantifies the amount of viruses infected in cells.

Match the virus with the appropriate antiviral agent. -Ribavirin

The only virus that has been globally eradicated is:

The recombinant virus assay (RVA)uses which type of amplification methodology?

Match the virus with the appropriate antiviral agent. -Amantadine

Match the assay with its function. -Inhibits viral DNA synthesis.

Match the assay with its function. -Quantifies sequence variation.

Match the assay with its function. -Inhibits viral plaque formation.

Match the virus with the appropriate antiviral agent. -Emtricitabine

Match the virus with the appropriate antiviral agent. -Acyclovir

If a physician prescribes Tamiflu to a patient,the patient is most likely infected with which virus?

When performing antiviral susceptibility testing,a standard inoculum must be used.If the inoculum quantity is too large,then the isolate will appear:

Match the assay with its function. -Viable cells take up neutral red dye.

Point mutations in the hemagglutination (HA)or neuraminidase (NA)genes during viral ribonucleoprotein (RNP)replication give rise to new strains.This mechanism is known as the antigenic:

Match the assay with its function. -Detects resistance genes.

Which type of assay uses a variety of endpoint measurements to determine whether the virus is inhibited by the antiviral drug or is demonstrating drug resistance?

Match the virus with the appropriate antiviral agent. -Lamivudine

At the end of the 2008-2009 influenza season,100% of influenza A H3N2 and the novel 2009 influenza A H1N1 were resistant to:

Microbial Taxonomy

Bacterial Genetics, metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Overview of Immunochemical Methods Used for Organism Detection

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Overview and General Considerations

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Overview of the Methods and Strategies in Parasitology

Intestinal Protozoa

Blood and Tissue Protozoa

Protozoa From Other Body Sites

Intestinal Nematodes

Tissue Nematodes

Blood Nematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Zygomycetes, Dermatophytes, and Opportunistic and Systemic Mycoses

Dematiaceious Molds

Opportunistic Atypical Fungus: Pjirovecii

The Yeasts