Question 1

To isolate anaerobic gram-positive organisms,which of the following media should be used?

Accepted Answer

A) Anaerobic blood agar 
B) Cycloserine cefoxitin fructose agar (CCF
A)  
C) Laked kanamycin-vancomycin (LKV)blood agar 
D) Anaerobic phenylethyl alcohol agar (PE
A)  
A) Anaerobic blood agar 
B) Cycloserine cefoxitin fructose agar (CCF
A)  
C) Laked kanamycin-vancomycin (LKV)blood agar 
D) Anaerobic phenylethyl alcohol agar (PE
A)  D

Question 2

Aspiration of peritoneal fluid using a needle and syringe is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable. A

Question 3

Feces in a sterile specimen cup is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable. B

Question 4

Match the media with its primary purpose.
-Thioglycollate broth

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 5

Percutaneous lung aspirate is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable.

Question 6

Match the media with its primary purpose.
-LKV blood agar

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 7

Match the media with its primary purpose.
-Egg-yolk agar

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 8

During specimen collection,an important thing to remember when handling a collection device with an oxygen-free atmosphere is to:

Accepted Answer

A) take care not to tip the container. 
B) hold the collected specimen in the refrigerator until processing. 
C) hold the collected specimen in the incubator until processing. 
D) avoid opening the container in an aerobic atmosphere. 
A) take care not to tip the container. 
B) hold the collected specimen in the refrigerator until processing. 
C) hold the collected specimen in the incubator until processing. 
D) avoid opening the container in an aerobic atmosphere.

Question 9

Prereduced,anaerobically sterilized (PRAS)media:

Accepted Answer

A) are produced,packaged,shipped,and stored under anaerobic conditions. 
B) have a short shelf life of only 2 weeks. 
C) enable the culture to be incubated in the regular incubator under aerobic conditions. 
D) contain peroxides that enhance the growth of certain anaerobes. 
A) are produced,packaged,shipped,and stored under anaerobic conditions. 
B) have a short shelf life of only 2 weeks. 
C) enable the culture to be incubated in the regular incubator under aerobic conditions. 
D) contain peroxides that enhance the growth of certain anaerobes.

Question 10

The final fill of the anaerobe jar prepared by the evacuation-replacement method consists of:

Accepted Answer

A) 20% to 30% nitrogen,5% to 10% hydrogen,and 70% to 80% CO₂. 
B) 80% to 90% oxygen,5% to 10% hydrogen,and 5% to 10% CO₂. 
C) 80% to 90% nitrogen,5% to 10% hydrogen,and 5% to 10% CO₂. 
D) 80% to 90% nitrogen,5% to 10% oxygen,and 5% to 10% CO₂. 
A) 20% to 30% nitrogen,5% to 10% hydrogen,and 70% to 80% CO₂. 
B) 80% to 90% oxygen,5% to 10% hydrogen,and 5% to 10% CO₂. 
C) 80% to 90% nitrogen,5% to 10% hydrogen,and 5% to 10% CO₂. 
D) 80% to 90% nitrogen,5% to 10% oxygen,and 5% to 10% CO₂.

Question 11

An acceptable specimen for anaerobic culture is:

Accepted Answer

A) nasopharyngeal swab. 
B) tissue obtained at biopsy. 
C) small bowel contents. 
D) catheterized urine. 
A) nasopharyngeal swab. 
B) tissue obtained at biopsy. 
C) small bowel contents. 
D) catheterized urine.

Question 12

Nasopharyngeal swab is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable.

Question 13

Cervical swab is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable.

Question 14

Match the media with its primary purpose.
-CCFA

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 15

Urine via percutaneous aspiration is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable.

Question 16

Bronchial washing collected with a double lumen-plugged catheter is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable.

Question 17

Match the media with its primary purpose.
-PEA agar

Accepted Answer

A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile 
A) Isolates of Prevotella and Bacteroides spp. 
B) Inhibits gram-negative rods 
C) Is nonselective;supports the growth of facultative anaerobes and aerobes 
D) Determines lecithinase and lipase production of Clostridia and Fusobacteria 
E) Is selective for Clostridium difficile

Question 18

Expectorated sputum is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable.

Question 19

An anaerobic media,which contains ferric ammonium citrate and hemin,bile salts,and gentamicin,is used to isolate which group of organisms?

Accepted Answer

A) Bacteroides spp. 
B) Clostridia spp. 
C) Actinomyces spp. 
D) Veillonella spp. 
A) Bacteroides spp. 
B) Clostridia spp. 
C) Actinomyces spp. 
D) Veillonella spp.

Question 20

Rectal swab is:

Accepted Answer

A) acceptable. 
B) not acceptable. 
A) acceptable. 
B) not acceptable.

Exam 39: Overview and General Considerations

To isolate anaerobic gram-positive organisms,which of the following media should be used?

Aspiration of peritoneal fluid using a needle and syringe is:

Feces in a sterile specimen cup is:

Match the media with its primary purpose. -Thioglycollate broth

Percutaneous lung aspirate is:

Match the media with its primary purpose. -LKV blood agar

Match the media with its primary purpose. -Egg-yolk agar

During specimen collection,an important thing to remember when handling a collection device with an oxygen-free atmosphere is to:

Prereduced,anaerobically sterilized (PRAS)media:

The final fill of the anaerobe jar prepared by the evacuation-replacement method consists of:

An acceptable specimen for anaerobic culture is:

Nasopharyngeal swab is:

Cervical swab is:

Match the media with its primary purpose. -CCFA

Urine via percutaneous aspiration is:

Bronchial washing collected with a double lumen-plugged catheter is:

Match the media with its primary purpose. -PEA agar

Expectorated sputum is:

An anaerobic media,which contains ferric ammonium citrate and hemin,bile salts,and gentamicin,is used to isolate which group of organisms?

Rectal swab is:

Microbial Taxonomy

Bacterial Genetics, metabolism, and Structure

Host-Microorganism Interactions

Laboratory Safety

Specimen Management

Role of Microscopy

Traditional Cultivation and Identification

Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Overview of Immunochemical Methods Used for Organism Detection

Principles of Antimicrobial Action Resistance

Laboratory Methods and Strategies for Antimicrobial Susceptibility Testing

Staphylococcus, Micrococcus, and Similar Organisms

Streptococcus, Enterococcus, and Similar Organisms

Bacillus and Similar Organisms

Listeria Corynebacterium and Similar Organisms

Erysipelothrix, Lactobacillus, and Similar Organisms

Nocardia, Streptomyces, Rhodococcus, Oerskovia, and Similar Organisms

Enterobacteriaceae

Acinetobacter, Stenotrophomonas, and Other Organisms

Pseudomonas, Burkholderia, and Similar Organisms

Achromobacter, Rhizobium, Ochrobactrum, and Similar Organisms

Chryseobacterium, Sphingobacterium, and Similar Organisms

Alcaligenes, Bordetella Nonpertussis, Comamonas, and Similar Organisms

Vibrio, aeromonas, Plesiomonas Shigelloides, and Chromobacterium Violaceum

Sphingomonas Paucimobilis and Similar Organisms

Moraxella

Eikenella Corrodens and Similar Organisms

Pasteurella and Similar Organisms

Actinobacillus, Kingella, Cardiobacterium, Capnocytophaga, and Similar Organisms

Haemophilus

Bartonella and Afipia

Campylobacter, Arcobacter, and Helicobacter

Legionella

Brucella

Bordetella Pertussis and Bordetella Parapertussis

Francisella

Streptobacillus Moniliformis and Spirillum Minus

Neisseria and Moraxella Catarrhalis

Laboratory Considerations

Mycobacteria

Obligate Intracellular and Non-culturable Bacterial Agents

Cell Walldeficient Bacteria: Mycoplasma and Ureaplasma

The Spirochetes

Overview of the Methods and Strategies in Parasitology

Intestinal Protozoa

Blood and Tissue Protozoa

Protozoa From Other Body Sites

Intestinal Nematodes

Tissue Nematodes

Blood Nematodes

Intestinal Cestodes

Tissue Cestodes

Intestinal Trematodes

Liver and Lung Trematodes

Blood Trematodes

Overview of Fungal Identification Methods and Strategies

Hyaline Molds, Zygomycetes, Dermatophytes, and Opportunistic and Systemic Mycoses

Dematiaceious Molds

Opportunistic Atypical Fungus: Pjirovecii