Exam 12: DNA Technology and Genomics

Which of the following is an example of a transgenic organism?

What is the preferred name of the technique used to determine if DNA comes from a particular individual?

TPOX is one of the STRs that is used to compare DNA between different people. Why is TPOX useful for comparing DNA between different people?

Genetically modifying ________ cells may directly affect future generations.

An advantage of using reverse transcriptase to prepare a gene for cloning is that

Which of the following statements regarding repetitive DNA is false?

After reading the paragraph below, answer the questions that follow. Four decades after the end of the Vietnam War, the remains of an Air Force pilot were discovered and returned to the United States. A search of Air Force records identified three families to which the remains might possibly belong. Each family had a surviving twin of a missing service member. The following STR profiles were obtained from the remains of the pilot and the surviving twins from the three families. -In order to match the pilot's remains to the correct family using DNA profiling,

When DNA from two sources is combined into one single piece of DNA, it is known as

What is the current standard tool used for DNA profiling by forensic scientists?

Below is a figure depicting the whole-genome shotgun method. Which step is most similar to a step used when preparing a genomic library?

During the process of electrophoresis, the ________ functions like a molecular sieve, separating the samples according to their size.

After reading the paragraph below, answer the questions that follow. Four decades after the end of the Vietnam War, the remains of an Air Force pilot were discovered and returned to the United States. A search of Air Force records identified three families to which the remains might possibly belong. Each family had a surviving twin of a missing service member. The following STR profiles were obtained from the remains of the pilot and the surviving twins from the three families. -Based on analysis of the STR sites shown, does the missing pilot belong to any of these three families?

Cystic fibrosis is a genetic disease that results from a defective CFTR protein that alters ion flow through the cell membrane such that water does not cross the cell membrane. Gene therapy is being used to attempt to help cystic fibrosis patients. Which of the following steps is not needed to develop a gene therapy treatment for cystic fibrosis?

You are setting up a PCR reaction and add a primer for one end of the target sequence, but you forget to add the primer for the other end of the target sequence. If you added the other necessary components, what do you expect to observe after running the PCR reaction for 40 cycles?

The type of repetitive DNA composed of sequences of large repeated units is often associated with

________ are a major source of restriction enzymes.

Biotechnology

Biotechnology companies sell kits that allow you to do PCR to amplify STR regions from human blood samples that you have collected. What components would you expect to find in the kit in order to be able to do successful PCR reactions?