Question 1

A small percentage of the total RNA in a cell is ribosomal RNA.

Accepted Answer

A) True 
 B)False

Question 2

Rho-dependent termination requires exposure of single-stranded RNA.

Accepted Answer

A) True 
 B)False

Question 3

Which of the following is true about transcription?

Accepted Answer

A) A repressor protein activates transcription of a negatively regulated gene. 
B) A positively regulated gene can be transcribed only in the absence of active repressor. 
C) In the absence of an activator,a negatively regulated gene is poorly transcribed. 
D) The initiation of transcription of regulated genes is controlled by regulatory proteins which bind to specific DNA sequences. 
E) All of the above. 
A) A repressor protein activates transcription of a negatively regulated gene. 
B) A positively regulated gene can be transcribed only in the absence of active repressor. 
C) In the absence of an activator,a negatively regulated gene is poorly transcribed. 
D) The initiation of transcription of regulated genes is controlled by regulatory proteins which bind to specific DNA sequences. 
E) All of the above.

Question 4

A gene contains two 100 bp introns and three 50 bp exons.About how long is the mature RNA transcribed from this gene?

Accepted Answer

A) 75 bp. 
B) 150 bp. 
C) 200 bp. 
D) 350 bp. 
E) Cannot tell from the information given. 
A) 75 bp. 
B) 150 bp. 
C) 200 bp. 
D) 350 bp. 
E) Cannot tell from the information given.

Question 5

Which of the following are correct matches for the given genes?

Accepted Answer

A) rpoN : nitrogen metabolism. 
B) rpoH : heat shock. 
C) Gene 55 : bacteriophage T4. 
D) All of the above. 
A) rpoN : nitrogen metabolism. 
B) rpoH : heat shock. 
C) Gene 55 : bacteriophage T4. 
D) All of the above.

Question 6

Mutations in sequences that are removed from the primary RNA transcript never affect RNA processing.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following is/are true concerning TAFs and TBP?

Accepted Answer

A) They are subunits of TFIIB and TFIID,respectively. 
B) TBP is required to initiate transcription of class I and class III genes. 
C) They are not associated with RNA polymerase I and RNA polymerase III. 
D) All of the above. 
A) They are subunits of TFIIB and TFIID,respectively. 
B) TBP is required to initiate transcription of class I and class III genes. 
C) They are not associated with RNA polymerase I and RNA polymerase III. 
D) All of the above.

Question 8

Rho-dependent termination

Accepted Answer

A) is due to destabilization of the RNA-DNA hybrid. 
B) is due to indirect contact between the transcription complex and rho as rho binds RNA. 
C) may cause the RNA polymerase to bind to template DNA. 
D) All of the above. 
A) is due to destabilization of the RNA-DNA hybrid. 
B) is due to indirect contact between the transcription complex and rho as rho binds RNA. 
C) may cause the RNA polymerase to bind to template DNA. 
D) All of the above.

Question 9

Which of the following is true for the unwinding of the DNA helix and the synthesis of a short stretch of RNA?

Accepted Answer

A) The process requires a helicase and a primase as in DNA replication. 
B) In transcription,these steps proceed via an RNA polymerase holoenzyme. 
C) The unwinding and subsequent synthesis above serve as a primer for chain elongation. 
D) All of the above. 
A) The process requires a helicase and a primase as in DNA replication. 
B) In transcription,these steps proceed via an RNA polymerase holoenzyme. 
C) The unwinding and subsequent synthesis above serve as a primer for chain elongation. 
D) All of the above.

Question 10

The product of the lacZ gene is ________.

Accepted Answer

A) lac repressor 
B) β-galactosidase 
C) thiogalactoside transacetylase 
D) lactose permease 
A) lac repressor 
B) β-galactosidase 
C) thiogalactoside transacetylase 
D) lactose permease

Question 11

RNA polymerase uses the ________ strand of DNA in transcription.

Accepted Answer

A) sense 
B) antisense 
C) coding 
D) uncoded 
A) sense 
B) antisense 
C) coding 
D) uncoded

Question 12

The large RNA-protein complex that processes introns and exons is called ________.

Accepted Answer

A) the replisome 
B) the catalytic RNA complex 
C) the spliceosome 
D) RNA ligase 
A) the replisome 
B) the catalytic RNA complex 
C) the spliceosome 
D) RNA ligase

Question 13

RNA polymerase has a higher error rate (one mutation for every 1 million nucleotides incorporated)than DNA polymerase because RNA polymerase does not have exonuclease proofreading activity but DNA polymerase does.

Accepted Answer

A) True 
 B)False

Question 14

Weak promoters contain

Accepted Answer

A) no consensus sequences. 
B) sequences that match consensus sequences poorly. 
C) no TATA box or -35 regions. 
D) TAT boxes but no -35 regions. 
A) no consensus sequences. 
B) sequences that match consensus sequences poorly. 
C) no TATA box or -35 regions. 
D) TAT boxes but no -35 regions.

Question 15

The type of RNA that is least stable in cells is ________.

Accepted Answer

A) tRNA 
B) rRNA 
C) mRNA 
D) small RNA 
A) tRNA 
B) rRNA 
C) mRNA 
D) small RNA

Question 16

The genes present vary among cell types,for example,pancreatic cells carry the gene for insulin,but skin cells do not.

Accepted Answer

A) True 
 B)False

Question 17

Rho-dependent termination occurs at pause sites within the coding region.

Accepted Answer

A) True 
 B)False

Question 18

The CRP-cAMP complex is an activator of the lac operon.

Accepted Answer

A) True 
 B)False

Question 19

Which statement about RNA polymerase is false?

Accepted Answer

A) The large beta subunit comprises the active site of the enzyme. 
B) The alpha subunits are the scaffold for assembly of the other subunits. 
C) The role of the omega subunit is well characterized. 
D) All of the above are false. 
A) The large beta subunit comprises the active site of the enzyme. 
B) The alpha subunits are the scaffold for assembly of the other subunits. 
C) The role of the omega subunit is well characterized. 
D) All of the above are false.

Question 20

The σ70 subunit in E.coli RNA polymerase acts as a(n)________.

Accepted Answer

A) repressor 
B) sliding clamp 
C) helicase 
D) initiation factor 
A) repressor 
B) sliding clamp 
C) helicase 
D) initiation factor

Exam 21: Transcription and RNA Processing

A small percentage of the total RNA in a cell is ribosomal RNA.

Rho-dependent termination requires exposure of single-stranded RNA.

Which of the following is true about transcription?

A gene contains two 100 bp introns and three 50 bp exons.About how long is the mature RNA transcribed from this gene?

Which of the following are correct matches for the given genes?

Mutations in sequences that are removed from the primary RNA transcript never affect RNA processing.

Which of the following is/are true concerning TAFs and TBP?

Rho-dependent termination

Which of the following is true for the unwinding of the DNA helix and the synthesis of a short stretch of RNA?

The product of the lacZ gene is ________.

RNA polymerase uses the ________ strand of DNA in transcription.

The large RNA-protein complex that processes introns and exons is called ________.

RNA polymerase has a higher error rate (one mutation for every 1 million nucleotides incorporated)than DNA polymerase because RNA polymerase does not have exonuclease proofreading activity but DNA polymerase does.

Weak promoters contain

The type of RNA that is least stable in cells is ________.

The genes present vary among cell types,for example,pancreatic cells carry the gene for insulin,but skin cells do not.

Rho-dependent termination occurs at pause sites within the coding region.

The CRP-cAMP complex is an activator of the lac operon.

Which statement about RNA polymerase is false?

The σ70 subunit in E.coli RNA polymerase acts as a(n)________.

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Atp Synthesis

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

DNA Replication, repair, and Recombination

Protein Synthesis

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