Question 1

Which statement is not true about the TATA box?

Accepted Answer

A) The TBP portion of the general transcription factor TFIID binds to the TATA box forming a molecular clamp. 
B) It is located about 19-27 bp upstream from the transcription start site of class II genes. 
C) Its sequence is highly conserved among all species. 
D) This region of DNA is more easily unwound to an open complex than other general sections of DNA. 
A) The TBP portion of the general transcription factor TFIID binds to the TATA box forming a molecular clamp. 
B) It is located about 19-27 bp upstream from the transcription start site of class II genes. 
C) Its sequence is highly conserved among all species. 
D) This region of DNA is more easily unwound to an open complex than other general sections of DNA.

Question 2

Which of the following is an incorrect association between RNA polymerase and its products?

Accepted Answer

A) RNA polymerase I : 35 - 47S pre-rRNA. 
B) RNA polymerase I: 5S rRNA. 
C) RNA polymerase II : mRNA precursors. 
D) RNA polymerase III : tRNA. 
E) None of the above. 
A) RNA polymerase I : 35 - 47S pre-rRNA. 
B) RNA polymerase I: 5S rRNA. 
C) RNA polymerase II : mRNA precursors. 
D) RNA polymerase III : tRNA. 
E) None of the above.

Question 3

Transcription is the process by which the information in mRNA molecules is interpreted and the appropriate proteins synthesized.

Accepted Answer

A) True 
 B)False

Question 4

Which subunit of the E.coli core RNA polymerase (a2bb'w)contributes to DNA binding?

Accepted Answer

A) a 
B) b 
C) b' 
D) w 
A) a 
B) b 
C) b' 
D) w

Question 5

A consensus sequence

Accepted Answer

A) may be a hypothetical sequence. 
B) indicates the nucleotides most commonly found at each position. 
C) describes the most efficient promoter sequence for the RNA polymerase holoenzyme. 
D) All of the above. 
A) may be a hypothetical sequence. 
B) indicates the nucleotides most commonly found at each position. 
C) describes the most efficient promoter sequence for the RNA polymerase holoenzyme. 
D) All of the above.

Question 6

Genes that encode for proteins that are needed for basic metabolic processes such as glycolysis are called ________ genes.

Accepted Answer

A) essential 
B) housekeeping 
C) core 
D) cardinal 
A) essential 
B) housekeeping 
C) core 
D) cardinal

Question 7

Which of the following is/are incorrect?

Accepted Answer

A) tRNAs are extremely stable molecules. 
B) Eukaryotic mRNA is highly stable. 
C) Bacterial mRNA is not rapidly degraded. 
D) All of the above. 
A) tRNAs are extremely stable molecules. 
B) Eukaryotic mRNA is highly stable. 
C) Bacterial mRNA is not rapidly degraded. 
D) All of the above.

Question 8

Which is not generally associated with RNA processing?

Accepted Answer

A) Addition of nucleotides. 
B) Removal of nucleotides. 
C) Covalent modification of bases. 
D) Phosphorylation at the 3' end. 
A) Addition of nucleotides. 
B) Removal of nucleotides. 
C) Covalent modification of bases. 
D) Phosphorylation at the 3' end.

Question 9

Operons are a common gene arrangement found in all living organisms.

Accepted Answer

A) True 
 B)False

Question 10

Which does not apply to the post-transcriptional processing of ribosomal RNA?

Accepted Answer

A) The subunits of rRNA are produced in equal amounts since they originate from the same transcript. 
B) tRNA precursors are sometimes cleaved from the primary transcript of rRNA precursor. 
C) A polyA tail is added to the 5' end of the 30S subunit. 
D) After initial cleavage of the primary transcript,specific endonucleases trim nucleotides from the ends of rRNA molecules. 
A) The subunits of rRNA are produced in equal amounts since they originate from the same transcript. 
B) tRNA precursors are sometimes cleaved from the primary transcript of rRNA precursor. 
C) A polyA tail is added to the 5' end of the 30S subunit. 
D) After initial cleavage of the primary transcript,specific endonucleases trim nucleotides from the ends of rRNA molecules.

Question 11

Which is not a property of RNA polymerase?

Accepted Answer

A) Polymerizes in the 5' → 3' direction. 
B) Catalyzes the formation of phosphodiester linkages. 
C) Polymerizes a reaction assisted by pyrophosphate hydrolysis. 
D) Has exonuclease proof-reading activity. 
A) Polymerizes in the 5' → 3' direction. 
B) Catalyzes the formation of phosphodiester linkages. 
C) Polymerizes a reaction assisted by pyrophosphate hydrolysis. 
D) Has exonuclease proof-reading activity.

Question 12

The purpose of the GTP cap on mRNA is ________.

Accepted Answer

A) to prevent the formation of hairpin loops 
B) to serve as a site for binding to the ribosome 
C) to assist in the transport of mRNA out of the nucleus 
D) to prevent exonuclease attack 
A) to prevent the formation of hairpin loops 
B) to serve as a site for binding to the ribosome 
C) to assist in the transport of mRNA out of the nucleus 
D) to prevent exonuclease attack

Question 13

Which statement is not true about the binding of TBP (TATA binding protein)?

Accepted Answer

A) The TBP subunit is only required for the initiation of transcription of class II genes. 
B) The binding of TBP to DNA distorts the DNA out of the standard B-DNA form. 
C) Contacts between TBP and DNA are mainly in the minor groove. 
D) TBP forms a saddle-shaped molecular clamp that surrounds DNA at the TATA box. 
A) The TBP subunit is only required for the initiation of transcription of class II genes. 
B) The binding of TBP to DNA distorts the DNA out of the standard B-DNA form. 
C) Contacts between TBP and DNA are mainly in the minor groove. 
D) TBP forms a saddle-shaped molecular clamp that surrounds DNA at the TATA box.

Question 14

Which states an improper location or activity?

Accepted Answer

A) RPo-RNA polymerase at a transcription bubble of DNA. 
B) RPc-RNA polymerase bound to single-stranded DNA. 
C) NusA -helps convert RNA polymerase to an elongation form. 
D) s-protein factor aiding promoter binding. 
A) RPo-RNA polymerase at a transcription bubble of DNA. 
B) RPc-RNA polymerase bound to single-stranded DNA. 
C) NusA -helps convert RNA polymerase to an elongation form. 
D) s-protein factor aiding promoter binding.

Question 15

Consensus sequences are found in TATA box and - 35 regions of promoters that are strong but not in those that are weak.

Accepted Answer

A) True 
 B)False

Question 16

When glucose is not available,

Accepted Answer

A) phosphoenolpyruvate forms and enters glycolysis. 
B) cyclic AMP levels decrease after inactivation of adenyl cyclase. 
C) cyclic AMP levels increase after activation of adenyl cyclase. 
D) ATP is not converted to cAMP. 
A) phosphoenolpyruvate forms and enters glycolysis. 
B) cyclic AMP levels decrease after inactivation of adenyl cyclase. 
C) cyclic AMP levels increase after activation of adenyl cyclase. 
D) ATP is not converted to cAMP.

Question 17

The splicing of exons does not occur in prokaryotes.

Accepted Answer

A) True 
 B)False

Question 18

Post-transcriptional modification of mRNA in eukaryotes occurs after they are transported to the cytosol,but before they bind to the ribosome.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following is correct?

Accepted Answer

A) Transcription proceeds in the 5' → 3' direction. 
B) The template strand is copied from the 5' end to the 3' end. 
C) RNA polymerization proceeds in the 3' → 5' direction. 
D) None of the above. 
A) Transcription proceeds in the 5' → 3' direction. 
B) The template strand is copied from the 5' end to the 3' end. 
C) RNA polymerization proceeds in the 3' → 5' direction. 
D) None of the above.

Question 20

What modification often occurs to the GTP cap on mRNA?

Accepted Answer

A) Amination. 
B) Deamination. 
C) Methylation. 
D) Demethylation. 
A) Amination. 
B) Deamination. 
C) Methylation. 
D) Demethylation.

Exam 21: Transcription and RNA Processing

Which statement is not true about the TATA box?

Which of the following is an incorrect association between RNA polymerase and its products?

Transcription is the process by which the information in mRNA molecules is interpreted and the appropriate proteins synthesized.

Which subunit of the E.coli core RNA polymerase (a2bb'w)contributes to DNA binding?

A consensus sequence

Genes that encode for proteins that are needed for basic metabolic processes such as glycolysis are called ________ genes.

Which of the following is/are incorrect?

Which is not generally associated with RNA processing?

Operons are a common gene arrangement found in all living organisms.

Which does not apply to the post-transcriptional processing of ribosomal RNA?

Which is not a property of RNA polymerase?

The purpose of the GTP cap on mRNA is ________.

Which statement is not true about the binding of TBP (TATA binding protein)?

Which states an improper location or activity?

Consensus sequences are found in TATA box and - 35 regions of promoters that are strong but not in those that are weak.

When glucose is not available,

The splicing of exons does not occur in prokaryotes.

Post-transcriptional modification of mRNA in eukaryotes occurs after they are transported to the cytosol,but before they bind to the ribosome.

Which of the following is correct?

What modification often occurs to the GTP cap on mRNA?

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Atp Synthesis

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

DNA Replication, repair, and Recombination

Protein Synthesis

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