Question 1

Which is true about the GTP cap on mRNA?

Accepted Answer

A) The 5' carbon of the GTP sugar is connected via phosphates to the 5' end of the mRNA. 
B) The 5' carbon of the GTP sugar is connected via phosphates to the 3' end of the mRNA. 
C) The 3' carbon of the GTP sugar is connected via phosphates to the 5' end of the mRNA. 
D) The 3' carbon of the GTP sugar is connected via phosphates to the 3' end of the mRNA. 
A) The 5' carbon of the GTP sugar is connected via phosphates to the 5' end of the mRNA. 
B) The 5' carbon of the GTP sugar is connected via phosphates to the 3' end of the mRNA. 
C) The 3' carbon of the GTP sugar is connected via phosphates to the 5' end of the mRNA. 
D) The 3' carbon of the GTP sugar is connected via phosphates to the 3' end of the mRNA.

Question 2

Positive regulation of a gene requires ________.

Accepted Answer

A) an activator protein 
B) positive cooperativity 
C) an allosteric inducer 
D) the conversion of ATP to ADP and inorganic phosphate 
A) an activator protein 
B) positive cooperativity 
C) an allosteric inducer 
D) the conversion of ATP to ADP and inorganic phosphate

Question 3

Which is not a feature of eukaryotic mRNA processing?

Accepted Answer

A) Addition of a 5'-5' triphosphate linkage to GTP. 
B) Methylation of the terminal CCA triad. 
C) Addition of a polyA tail to the 3' end. 
D) Removal of intron sequences. 
A) Addition of a 5'-5' triphosphate linkage to GTP. 
B) Methylation of the terminal CCA triad. 
C) Addition of a polyA tail to the 3' end. 
D) Removal of intron sequences.

Question 4

A transcription complex does not form when one or more proteins bind to the promoter sequence and to RNA polymerase in eukaryotes.

Accepted Answer

A) True 
 B)False

Question 5

Transcription sometimes stops near pause sites because these are regions

Accepted Answer

A) rich in GC base pairs. 
B) rich in AT base pairs. 
C) without palindromic sequences. 
D) where Nus A is not present. 
A) rich in GC base pairs. 
B) rich in AT base pairs. 
C) without palindromic sequences. 
D) where Nus A is not present.

Question 6

RNA polymerase requires a high-precision fit when catalyzing the formation of a new phosphodiester linkage.

Accepted Answer

A) True 
 B)False

Question 7

Which is not true about the lac repressor?

Accepted Answer

A) Its gene is part of the lac operon and is transcribed along with lacZ,lacA and lacY. 
B) When it binds to DNA,it forms a loop-type structure. 
C) It likely blocks both RNA polymerase binding as well as transcription initiation. 
D) It binds to DNA by interaction of an α-helix with the major groove. 
A) Its gene is part of the lac operon and is transcribed along with lacZ,lacA and lacY. 
B) When it binds to DNA,it forms a loop-type structure. 
C) It likely blocks both RNA polymerase binding as well as transcription initiation. 
D) It binds to DNA by interaction of an α-helix with the major groove.

Question 8

TFIIB can bind to DNA in the absence of TFIID.

Accepted Answer

A) True 
 B)False

Question 9

Which has its primary transcript produced in the nucleolus?

Accepted Answer

A) tRNA 
B) mRNA 
C) rRNA 
D) sRNA 
A) tRNA 
B) mRNA 
C) rRNA 
D) sRNA

Question 10

What does it mean to say that the most common type of promoter in E.coli is bipartite?

Accepted Answer

A) The promoter region is divided into two parts which flank the sequence for the gene. 
B) There are two separate regions of sequence similarity in the promoter. 
C) The sequence of the promoter has dyad symmetry. 
D) The promoter must bind two RNA polymerase holoenzymes to transcribe the gene. 
A) The promoter region is divided into two parts which flank the sequence for the gene. 
B) There are two separate regions of sequence similarity in the promoter. 
C) The sequence of the promoter has dyad symmetry. 
D) The promoter must bind two RNA polymerase holoenzymes to transcribe the gene.

Question 11

RNase D is an endonuclease that adds CCA to the 3' end of tRNA.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following is an incorrect association between transcription factors and their characteristics?

Accepted Answer

A) TFIIB : interacts with RNA polymerase II. 
B) SP1: binds to GC-rich sequence. 
C) TFIIH: couples transcription to DNA repair. 
D) TFIIE : recognizes the core sequence CCAAT. 
E) All of the above. 
A) TFIIB : interacts with RNA polymerase II. 
B) SP1: binds to GC-rich sequence. 
C) TFIIH: couples transcription to DNA repair. 
D) TFIIE : recognizes the core sequence CCAAT. 
E) All of the above.

Question 13

Protein X is found to bind to a certain gene's promoter only when substance A is not present.The gene can be transcribed in the presence of both X and A.It is not transcribed in the presence of X alone.Protein X is a(n)________.Substance A is a(n)________.

Accepted Answer

A) repressor;inducer 
B) repressor;corepressor 
C) activator;inducer 
D) activator;corepressor 
A) repressor;inducer 
B) repressor;corepressor 
C) activator;inducer 
D) activator;corepressor

Question 14

The same RNA polymerase that transcribes the ribosomal RNA transcribes all mitochondrial genes.

Accepted Answer

A) True 
 B)False

Question 15

Which of the following is true about transcription?

Accepted Answer

A) Promoters are regions of DNA which serve as transcription initiation sites. 
B) Initiation is the rate-limiting step in transcription. 
C) Each gene,in eukaryotic cells,usually has its own promoter. 
D) All of the above. 
A) Promoters are regions of DNA which serve as transcription initiation sites. 
B) Initiation is the rate-limiting step in transcription. 
C) Each gene,in eukaryotic cells,usually has its own promoter. 
D) All of the above.

Question 16

An operon is ________.

Accepted Answer

A) a set of genes whose transcription is controlled by a single promoter 
B) the site of repressor binding 
C) a protein that activates the transcription of a whole class of genes 
D) a multisubunit complex responsible for the splicing of exons 
A) a set of genes whose transcription is controlled by a single promoter 
B) the site of repressor binding 
C) a protein that activates the transcription of a whole class of genes 
D) a multisubunit complex responsible for the splicing of exons

Question 17

Which statement is false about the utilization of lactose as a carbon source by E.coli?

Accepted Answer

A) In the presence of both glucose and lactose,the enzymes necessary for lactose catabolism are produced only in limited amounts. 
B) Lactose is the inducer for the enzymes responsible for its own catabolism. 
C) In the absence of lactose,the repressor binds to a single site upstream of the lac genes. 
D) The catabolic enzymes are encoded in a three-gene operon controlled by a single promoter. 
A) In the presence of both glucose and lactose,the enzymes necessary for lactose catabolism are produced only in limited amounts. 
B) Lactose is the inducer for the enzymes responsible for its own catabolism. 
C) In the absence of lactose,the repressor binds to a single site upstream of the lac genes. 
D) The catabolic enzymes are encoded in a three-gene operon controlled by a single promoter.

Question 18

The unwinding of DNA at the initiation site is an example of a conformational change.

Accepted Answer

A) True 
 B)False

Question 19

Which statement is false concerning the general transcription factor,TFIID?

Accepted Answer

A) It binds to the promoter. 
B) Its TBP subunit binds to the region containing the TATA box. 
C) It interacts with TFIIA in the presence of DNA. 
D) It is the RNA polymerase II initiation factor. 
E) None of the above. 
A) It binds to the promoter. 
B) Its TBP subunit binds to the region containing the TATA box. 
C) It interacts with TFIIA in the presence of DNA. 
D) It is the RNA polymerase II initiation factor. 
E) None of the above.

Question 20

Which statement is false about operons that have the feature of catabolic repression?

Accepted Answer

A) Several different multisubunit repressors can block the transcription of such an operon. 
B) Operons with catabolic repression have characteristically weak promoters. 
C) Their transcription is lowered in the presence of glucose. 
D) Transcription of such an operon is increased as the concentration of cAMP increases. 
A) Several different multisubunit repressors can block the transcription of such an operon. 
B) Operons with catabolic repression have characteristically weak promoters. 
C) Their transcription is lowered in the presence of glucose. 
D) Transcription of such an operon is increased as the concentration of cAMP increases.

Exam 21: Transcription and RNA Processing

Which is true about the GTP cap on mRNA?

Positive regulation of a gene requires ________.

Which is not a feature of eukaryotic mRNA processing?

A transcription complex does not form when one or more proteins bind to the promoter sequence and to RNA polymerase in eukaryotes.

Transcription sometimes stops near pause sites because these are regions

RNA polymerase requires a high-precision fit when catalyzing the formation of a new phosphodiester linkage.

Which is not true about the lac repressor?

TFIIB can bind to DNA in the absence of TFIID.

Which has its primary transcript produced in the nucleolus?

What does it mean to say that the most common type of promoter in E.coli is bipartite?

RNase D is an endonuclease that adds CCA to the 3' end of tRNA.

Which of the following is an incorrect association between transcription factors and their characteristics?

Protein X is found to bind to a certain gene's promoter only when substance A is not present.The gene can be transcribed in the presence of both X and A.It is not transcribed in the presence of X alone.Protein X is a(n)________.Substance A is a(n)________.

The same RNA polymerase that transcribes the ribosomal RNA transcribes all mitochondrial genes.

Which of the following is true about transcription?

An operon is ________.

Which statement is false about the utilization of lactose as a carbon source by E.coli?

The unwinding of DNA at the initiation site is an example of a conformational change.

Which statement is false concerning the general transcription factor,TFIID?

Which statement is false about operons that have the feature of catabolic repression?

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Atp Synthesis

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

DNA Replication, repair, and Recombination

Protein Synthesis

Filters

Protein X is found to bind to a certain gene's promoter only when substance A is not present.The gene can be transcribed in the presence of both X and A.It is not transcribed in the presence of X alone.Protein X is a(n).Substance A is a(n).