Question 1

The genes that encode proteins are transcribed by RNA polymerase ________.

Accepted Answer

A) I 
B) II 
C) III 
D) IV 
A) I 
B) II 
C) III 
D) IV B

Question 2

Which observation is not evidence that supports the theory that mitochondria and chloroplasts originate from symbiotic bacteria?

Accepted Answer

A) Genes for both mitochondrial and chloroplast RNA polymerase are encoded in the DNA of these organelles. 
B) Both mitochondria and chloroplasts are about the same size as bacterial cells. 
C) Chloroplast RNA polymerase is similar in sequence to that of cyanobacteria. 
D) Both mitochondria and chloroplasts have their own DNA. 
A) Genes for both mitochondrial and chloroplast RNA polymerase are encoded in the DNA of these organelles. 
B) Both mitochondria and chloroplasts are about the same size as bacterial cells. 
C) Chloroplast RNA polymerase is similar in sequence to that of cyanobacteria. 
D) Both mitochondria and chloroplasts have their own DNA. A

Question 3

The gene products of the lacZ,lacA and lacY genes are specific for the hydrolysis of a different β-galactoside sugar.

Accepted Answer

A) True 
 B)False  False

Question 4

The sigma-70 subunit

Accepted Answer

A) increases the affinity of the core polymerase for nonpromoter sequences. 
B) decreases the affinity of the core polymerase for specific promoter sequences. 
C) is responsible for transcribing many genes. 
D) is associated with a holoenzyme,enabling the formation of more stable complexes. 
E) All of the above. 
A) increases the affinity of the core polymerase for nonpromoter sequences. 
B) decreases the affinity of the core polymerase for specific promoter sequences. 
C) is responsible for transcribing many genes. 
D) is associated with a holoenzyme,enabling the formation of more stable complexes. 
E) All of the above.

Question 5

Eukaryotic RNA polymerases do not bind on their own to DNA templates but prokaryotic RNA polymerases do.

Accepted Answer

A) True 
 B)False

Question 6

Constitutive genes

Accepted Answer

A) have products that are maintained at constant levels. 
B) are genes expressed by all cells. 
C) are transcribed continuously. 
D) All of the above. 
E) None of the above. 
A) have products that are maintained at constant levels. 
B) are genes expressed by all cells. 
C) are transcribed continuously. 
D) All of the above. 
E) None of the above.

Question 7

When a sequence of double-strand DNA is displayed,

Accepted Answer

A) the coding strand is written in the 3' → 5' direction. 
B) the template strand is written in the 5' → 3' direction. 
C) the coding strand is identical to the RNA product. 
D) None of the above. 
A) the coding strand is written in the 3' → 5' direction. 
B) the template strand is written in the 5' → 3' direction. 
C) the coding strand is identical to the RNA product. 
D) None of the above.

Question 8

Activators and repressors are often allosteric proteins.

Accepted Answer

A) True 
 B)False

Question 9

For an RNA hairpin loop to form,the transcribed region of the DNA sequence must ________.

Accepted Answer

A) be a palindrome 
B) have dyad symmetry 
C) contain an intron 
D) have a tandem repeat 
A) be a palindrome 
B) have dyad symmetry 
C) contain an intron 
D) have a tandem repeat

Question 10

An extremely large lactose source is encountered by an E.coli cell in the lumen of the small intestine of a human who just ate an entire carton of ice cream.Which of the following would most likely be a contributor to why the E.coli does not metabolize any of the lactose?

Accepted Answer

A) Presence of allolactose. 
B) Presence of phosphorylated adenylate cyclase. 
C) Absence of glucose. 
D) Absence of cAMP. 
A) Presence of allolactose. 
B) Presence of phosphorylated adenylate cyclase. 
C) Absence of glucose. 
D) Absence of cAMP.

Question 11

Characteristics of ligands involved in transcription include:

Accepted Answer

A) They can modify the function of repressors and activators. 
B) They are considered inducers when they bind to repressors and activate them. 
C) They are considered corepressors when they bind to repressors and inactivate them. 
D) All of the above. 
A) They can modify the function of repressors and activators. 
B) They are considered inducers when they bind to repressors and activate them. 
C) They are considered corepressors when they bind to repressors and inactivate them. 
D) All of the above.

Question 12

In E.coli,all transcription termination requires a specific protein,rho.

Accepted Answer

A) True 
 B)False

Question 13

Regulation of gene expression can only occur at the level of transcription.

Accepted Answer

A) True 
 B)False

Question 14

Which is not part of the lac operon?

Accepted Answer

A) lacA 
B) lacI 
C) lacY 
D) lacZ 
E) None of the above;they are all part of the lac operon. 
A) lacA 
B) lacI 
C) lacY 
D) lacZ 
E) None of the above;they are all part of the lac operon.

Question 15

Class I genes encode large ribosomal RNA.

Accepted Answer

A) True 
 B)False

Question 16

Which statement is true about the relative number of accessory proteins necessary for RNA polymerization in eukaryotes versus E.coli?

Accepted Answer

A) Eukaryotes require many more accessory proteins. 
B) They require about the same number of accessory proteins. 
C) Eukaryotes require far fewer accessory proteins. 
D) The number required varies widely by species.Some eukaryotes require less than E.coli and some require more. 
A) Eukaryotes require many more accessory proteins. 
B) They require about the same number of accessory proteins. 
C) Eukaryotes require far fewer accessory proteins. 
D) The number required varies widely by species.Some eukaryotes require less than E.coli and some require more.

Question 17

Two types of ________ RNA are miRNA and siRNA.

Accepted Answer

A) transfer 
B) ribosomal 
C) messenger 
D) regulatory 
A) transfer 
B) ribosomal 
C) messenger 
D) regulatory

Question 18

All housekeeping genes have weak promoters and are regulated to maintain their gene products at constant low level concentrations.

Accepted Answer

A) True 
 B)False

Question 19

When a repressor protein is ________ (bound,unbound)to a promoter region transcription is allowed;when an activator protein is ________ (bound,unbound)to a promoter transcription is stimulated.

Accepted Answer

A) bound;unbound 
B) unbound;bound 
C) bound;bound 
D) unbound;unbound 
A) bound;unbound 
B) unbound;bound 
C) bound;bound 
D) unbound;unbound

Question 20

The genes encoding the chloroplast RNA polymerase are not similar in sequence to those of RNA polymerase in cyanobacteria.

Accepted Answer

A) True 
 B)False

Exam 21: Transcription and RNA Processing

The genes that encode proteins are transcribed by RNA polymerase ________.

Which observation is not evidence that supports the theory that mitochondria and chloroplasts originate from symbiotic bacteria?

The gene products of the lacZ,lacA and lacY genes are specific for the hydrolysis of a different β-galactoside sugar.

The sigma-70 subunit

Eukaryotic RNA polymerases do not bind on their own to DNA templates but prokaryotic RNA polymerases do.

Constitutive genes

When a sequence of double-strand DNA is displayed,

Activators and repressors are often allosteric proteins.

For an RNA hairpin loop to form,the transcribed region of the DNA sequence must ________.

An extremely large lactose source is encountered by an E.coli cell in the lumen of the small intestine of a human who just ate an entire carton of ice cream.Which of the following would most likely be a contributor to why the E.coli does not metabolize any of the lactose?

Characteristics of ligands involved in transcription include:

In E.coli,all transcription termination requires a specific protein,rho.

Regulation of gene expression can only occur at the level of transcription.

Which is not part of the lac operon?

Class I genes encode large ribosomal RNA.

Which statement is true about the relative number of accessory proteins necessary for RNA polymerization in eukaryotes versus E.coli?

Two types of ________ RNA are miRNA and siRNA.

All housekeeping genes have weak promoters and are regulated to maintain their gene products at constant low level concentrations.

When a repressor protein is ________ (bound,unbound)to a promoter region transcription is allowed;when an activator protein is ________ (bound,unbound)to a promoter transcription is stimulated.

The genes encoding the chloroplast RNA polymerase are not similar in sequence to those of RNA polymerase in cyanobacteria.

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Atp Synthesis

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

DNA Replication, repair, and Recombination

Protein Synthesis

Filters

When a repressor protein is (bound,unbound)to a promoter region transcription is allowed;when an activator protein is (bound,unbound)to a promoter transcription is stimulated.