Question 1

In the polymerase chain reaction

Accepted Answer

A)  it is possible to amplify small amounts of DNA without cloning. 
B)  conditions must be carefully controlled to prevent explosions. 
C)  reaction mixtures must be kept chilled at all times. 
D)  all of these 
E)  none of these 
A)  it is possible to amplify small amounts of DNA without cloning. 
B)  conditions must be carefully controlled to prevent explosions. 
C)  reaction mixtures must be kept chilled at all times. 
D)  all of these 
E)  none of these

Question 2

Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β -gal gene codes for the enzyme β -galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpR gene confers ampicillin resistance.   Refer to Exhibit 13D. Which restriction site is best for inserting a DNA fragment for analysis?

Accepted Answer

A)  Bam HI 
B)  Eco RI 
C)  Hind III 
D)  They're all equally good. 
A)  Bam HI 
B)  Eco RI 
C)  Hind III 
D)  They're all equally good.

Question 3

In DNA sequencing, fragments to be analyzed are produced by

Accepted Answer

A)  acid hydrolysis 
B)  base hydrolysis 
C)  selective interruption of DNA synthesis 
D)  exposure to 32P 
A)  acid hydrolysis 
B)  base hydrolysis 
C)  selective interruption of DNA synthesis 
D)  exposure to 32P

Question 4

Fluorescence and other luminescent methods of visualizing bands offer these advantages over methods dependent on radioactivity.

Accepted Answer

A)  They can detect smaller amounts of material (they are more sensitive). 
B)  Fluorescent molecules like ethidium bromide offer no health hazard, while radioisotopes do. 
C)  Special licenses are required to work with radioisotopes. 
D)  These methods are both more sensitive and require no special license. 
E)  All of these are advantages. 
A)  They can detect smaller amounts of material (they are more sensitive). 
B)  Fluorescent molecules like ethidium bromide offer no health hazard, while radioisotopes do. 
C)  Special licenses are required to work with radioisotopes. 
D)  These methods are both more sensitive and require no special license. 
E)  All of these are advantages.

Question 5

Fluorescence works because the fluorescent molecule

Accepted Answer

A)  absorbs light at one wavelength and emits light at a longer wavelength. 
B)  absorbs light at one wavelength and emits light at a shorter wavelength. 
C)  absorbs light at one wavelength and emits light at the same wavelength. 
D)  absorbs light at many wavelengths and emits light at many wavelengths. 
A)  absorbs light at one wavelength and emits light at a longer wavelength. 
B)  absorbs light at one wavelength and emits light at a shorter wavelength. 
C)  absorbs light at one wavelength and emits light at the same wavelength. 
D)  absorbs light at many wavelengths and emits light at many wavelengths.

Question 6

A plasmid is

Accepted Answer

A)  a virus that infects bacteria. 
B)  a piece of DNA derived from two or more sources. 
C)  a small circular DNA that is not part of a bacterial chromosome. 
D)  an artificially created cytoplasm. 
A)  a virus that infects bacteria. 
B)  a piece of DNA derived from two or more sources. 
C)  a small circular DNA that is not part of a bacterial chromosome. 
D)  an artificially created cytoplasm.

Question 7

Which of the following is true?

Accepted Answer

A)  When DNA evidence is used in crime cases, it is easier to prove that a person is guilty than that they are innocent. 
B)  When DNA evidence is used in a paternity case, it is easier to prove that a person is the father rather than the person is not the fathere 
C)  DNA evidence is easier to use to exclude a putative match than to include it 
D)  none of these is true 
A)  When DNA evidence is used in crime cases, it is easier to prove that a person is guilty than that they are innocent. 
B)  When DNA evidence is used in a paternity case, it is easier to prove that a person is the father rather than the person is not the fathere 
C)  DNA evidence is easier to use to exclude a putative match than to include it 
D)  none of these is true

Question 8

A single clone of interest can be distinguished from others in a mixture of clones by

Accepted Answer

A)  testing the clones for antibiotic resistance. 
B)  mobility of the clones in gel electrophoresis. 
C)  a specific probe, usually a labeled complementary DNA. 
D)  resistance to damage by ultraviolet light. 
A)  testing the clones for antibiotic resistance. 
B)  mobility of the clones in gel electrophoresis. 
C)  a specific probe, usually a labeled complementary DNA. 
D)  resistance to damage by ultraviolet light.

Question 9

Genetic engineering which recombines DNA from different species is

Accepted Answer

A)  a molecular extension of traditional cross-breeding methods. 
B)  only possible with plants. 
C)  only possible with animals. 
D)  only possible with bacteria. 
A)  a molecular extension of traditional cross-breeding methods. 
B)  only possible with plants. 
C)  only possible with animals. 
D)  only possible with bacteria.

Question 10

Genetic recombination occurs in nature.

Accepted Answer

A) True 
 B)False

Question 11

Cells that contain a "blue\white screening" plasmid that has an added gene are recognized by the method:

Accepted Answer

A)  Ability to grow on ampicillin. 
B)  Inability to grow on ampicillin. 
C)  The colonies have a blue color. 
D)  The colonies lack a blue color. 
E)  More than one of these choices indicates that the plasmid contains recombined DNA. 
A)  Ability to grow on ampicillin. 
B)  Inability to grow on ampicillin. 
C)  The colonies have a blue color. 
D)  The colonies lack a blue color. 
E)  More than one of these choices indicates that the plasmid contains recombined DNA.

Question 12

In a metabolic study using microarrays, a yellow dot represents the location of DNA on the microarray:

Accepted Answer

A)  for which mRNA was produced in both the control and the test case 
B)  for which no mRNA was produced 
C)  for which only the control case produced mRNA 
D)  for which only the test case produced mRNA 
A)  for which mRNA was produced in both the control and the test case 
B)  for which no mRNA was produced 
C)  for which only the control case produced mRNA 
D)  for which only the test case produced mRNA

Question 13

An isoschizomer is a(n)

Accepted Answer

A)  a DNA sequence that is identical to one in a different organsim 
B)  a DNA sequence from a virus that mimics a sequence in bacteria 
C)  enzyme that cuts DNA from the 3' end 
D)  a restriction enzyme that has the same sequence specificity as another restriction enzyme from a different organism 
E)  none of these is correct 
A)  a DNA sequence that is identical to one in a different organsim 
B)  a DNA sequence from a virus that mimics a sequence in bacteria 
C)  enzyme that cuts DNA from the 3' end 
D)  a restriction enzyme that has the same sequence specificity as another restriction enzyme from a different organism 
E)  none of these is correct

Question 14

A gene from Bacillus thuringiensis (Bt) is used to make plants resistant against all insects which might feed on the plant.

Accepted Answer

A) True 
 B)False

Question 15

Migration of DNA during electrophoresis is based

Accepted Answer

A)  mostly on the size of the molecule, since the ratio of charge to mass is approximately the same, no matter how large the DNA is. 
B)  mostly on the charge of the molecule, since the ratio of charge to mass is approximately the same, no matter how large the DNA is. 
C)  mostly on the charge-to-mass ratio since this can vary greatly among pieces of DNA. 
D)  on parameters not understood well. 
A)  mostly on the size of the molecule, since the ratio of charge to mass is approximately the same, no matter how large the DNA is. 
B)  mostly on the charge of the molecule, since the ratio of charge to mass is approximately the same, no matter how large the DNA is. 
C)  mostly on the charge-to-mass ratio since this can vary greatly among pieces of DNA. 
D)  on parameters not understood well.

Question 16

Which of the following components is responsible for interrupting the synthesis of DNA at every possible site in a population of molecules during nucleic acid base sequencing?

Accepted Answer

A)  2',3'-dideoxynucleotide 
B)  3',5'-cyclic nucleotide 
C)  Polynucleotide 
D)  N-nucleotide 
A)  2',3'-dideoxynucleotide 
B)  3',5'-cyclic nucleotide 
C)  Polynucleotide 
D)  N-nucleotide

Question 17

The "c" in cDNA stands for this word:

Accepted Answer

A)  Complete. 
B)  Circular. 
C)  Complementary. 
D)  Chromosomal. 
E)  Confusing. 
A)  Complete. 
B)  Circular. 
C)  Complementary. 
D)  Chromosomal. 
E)  Confusing.

Question 18

The "natural" function of restriction endonucleases is to

Accepted Answer

A)  protect bacterial cells from invasion by viruses (bacteriophages). 
B)  help bacteriophages infect cells. 
C)  regulate gene expression from specific promoters. 
D)  remove chromatin from histones. 
A)  protect bacterial cells from invasion by viruses (bacteriophages). 
B)  help bacteriophages infect cells. 
C)  regulate gene expression from specific promoters. 
D)  remove chromatin from histones.

Question 19

Which of the following conditions is not a requirement to assure production of a protein when a plasmid is being designed to express a eukaryotic protein in a bacterium?

Accepted Answer

A)  Introns must be deleted from the gene. 
B)  The cloning site must include an RNA polymerase promoter. 
C)  The mRNA product must contain a ribosome-binding site. 
D)  Differences in the genetic code between eukaryotes and prokaryotes must be accommodated. 
E)  All of these conditions must be met. 
A)  Introns must be deleted from the gene. 
B)  The cloning site must include an RNA polymerase promoter. 
C)  The mRNA product must contain a ribosome-binding site. 
D)  Differences in the genetic code between eukaryotes and prokaryotes must be accommodated. 
E)  All of these conditions must be met.

Question 20

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.   Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the Afl III site, the recombinant plasmid would lack which of the following properties?

Accepted Answer

A)  It can replicate autonomously. 
B)  It will be resistant to the antibiotic tetracycline. 
C)  It will be resistant to the antibiotic ampicillin. 
D)  It will possess at lest two promoter sites. 
E)  All of these are true for the recombined plasmid. 
A)  It can replicate autonomously. 
B)  It will be resistant to the antibiotic tetracycline. 
C)  It will be resistant to the antibiotic ampicillin. 
D)  It will possess at lest two promoter sites. 
E)  All of these are true for the recombined plasmid.

Exam 13: Nucleic Acid Biotechnology Techniques

In the polymerase chain reaction

In DNA sequencing, fragments to be analyzed are produced by

Fluorescence and other luminescent methods of visualizing bands offer these advantages over methods dependent on radioactivity.

Fluorescence works because the fluorescent molecule

A plasmid is

Which of the following is true?

A single clone of interest can be distinguished from others in a mixture of clones by

Genetic engineering which recombines DNA from different species is

Genetic recombination occurs in nature.

Cells that contain a "blue\white screening" plasmid that has an added gene are recognized by the method:

In a metabolic study using microarrays, a yellow dot represents the location of DNA on the microarray:

An isoschizomer is a(n)

A gene from Bacillus thuringiensis (Bt) is used to make plants resistant against all insects which might feed on the plant.

Migration of DNA during electrophoresis is based

Which of the following components is responsible for interrupting the synthesis of DNA at every possible site in a population of molecules during nucleic acid base sequencing?

The "c" in cDNA stands for this word:

The "natural" function of restriction endonucleases is to

Which of the following conditions is not a requirement to assure production of a protein when a plasmid is being designed to express a eukaryotic protein in a bacterium?

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the Afl III site, the recombinant plasmid would lack which of the following properties?

Biochemistry and the Organization of Cells

Water the Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins Enzymes

The Behavior of Proteins Enzymes Mechanisms and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids How Structure Conveys Information

Biosynthesis of Nucleic Acids Replication

Transcription of the Genetic Code the Biosynthesis of Rna

Protein Synthesis Translation of the Genetic Message

Viruses Cancer and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism Cellular Signaling

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