Question 1

If mRNA is converted to DNA, digested with restriction enzymes, and cloned into suitable vectors, the resultant library is called a:

Accepted Answer

A)  DNA library 
B)  cDNA library 
C)  mRNA library 
D)  none of these 
A)  DNA library 
B)  cDNA library 
C)  mRNA library 
D)  none of these

Question 2

A proteome is

Accepted Answer

A)  a protein-based vector. 
B)  a three-dimensional protein structure. 
C)  a collection of all the proteins produced in a given cell or tissue. 
D)  an improperly digested protein responsible for certain diseases such as "mad cow" disease. 
A)  a protein-based vector. 
B)  a three-dimensional protein structure. 
C)  a collection of all the proteins produced in a given cell or tissue. 
D)  an improperly digested protein responsible for certain diseases such as "mad cow" disease.

Question 3

One major obstacle to using bacteria to produce human proteins is because addition of sugar residues to the proteins, glycosylation, never occurs properly.

Accepted Answer

A) True 
 B)False

Question 4

Which of the following plants have been genetically modified?

Accepted Answer

A)  Soy beans 
B)  Corn. 
C)  Cotton 
D)  Tomatoes 
E)  All of these plants have been genetically modified. 
A)  Soy beans 
B)  Corn. 
C)  Cotton 
D)  Tomatoes 
E)  All of these plants have been genetically modified.

Question 5

When viruses are used as vectors in gene therapy

Accepted Answer

A)  they are not to transform cells taken from the patient's body to give back to the patient 
B)  they can cause unexpected difficulties 
C)  both of the above 
D)  neither of the above 
A)  they are not to transform cells taken from the patient's body to give back to the patient 
B)  they can cause unexpected difficulties 
C)  both of the above 
D)  neither of the above

Question 6

The RFLP technique

Accepted Answer

A)  is of most use with prokaryotic DNA 
B)  is of no use on DNA that contains introns 
C)  is used on the DNA of organisms that have two sets of chromosomes 
D)  provides fewer genetic markers than classical genetic techniques 
A)  is of most use with prokaryotic DNA 
B)  is of no use on DNA that contains introns 
C)  is used on the DNA of organisms that have two sets of chromosomes 
D)  provides fewer genetic markers than classical genetic techniques

Question 7

A feature of the Sanger-Coulson method for DNA sequencing is

Accepted Answer

A)  chemical modification of all bases 
B)  no need to use gel electrophoresis 
C)  use of triple-helical DNA 
D)  direct "reading" of the sequence of bases 
A)  chemical modification of all bases 
B)  no need to use gel electrophoresis 
C)  use of triple-helical DNA 
D)  direct "reading" of the sequence of bases

Question 8

A chance to be a forensic scientist. RFLP analysis has been requested by the prosecuting attorney in a murder case. DNA samples have been isolated from a blood sample at the crime scene (C), a hair follicle from the victim's hairbrush (V), and a mouth swab of the accused (A). The results of the test are shown. What would you tell the attorney?

Accepted Answer

A)  The results indicate the accused is guilty. 
B)  The results indicate the accused is innocent. 
C)  The results are inconclusive. 
D)  DNA fingerprinting has no place in forensic science 
E)  none of these 
A)  The results indicate the accused is guilty. 
B)  The results indicate the accused is innocent. 
C)  The results are inconclusive. 
D)  DNA fingerprinting has no place in forensic science 
E)  none of these

Question 9

The best primers for the PCR reaction have the following feature:

Accepted Answer

A)  They have a high G − C content. 
B)  They have a high A − T content. 
C)  They should be palindromic. 
D)  The AT\GC ratio does not matter. 
E)  They should anneal rapidly, before the larger DNA strands reanneal. 
A)  They have a high G − C content. 
B)  They have a high A − T content. 
C)  They should be palindromic. 
D)  The AT\GC ratio does not matter. 
E)  They should anneal rapidly, before the larger DNA strands reanneal.

Question 10

Forensic uses of DNA to identify victims or criminals exploit the following trait in DNA:

Accepted Answer

A)  Differences in sizes of DNA fragments (RFLPs). 
B)  Footprinting. 
C)  Site directed mutations. 
D)  DNA with proteins bound moves slower in gel electrophoresis. 
E)  More than one of these is important in using DNA for forensic identification. 
A)  Differences in sizes of DNA fragments (RFLPs). 
B)  Footprinting. 
C)  Site directed mutations. 
D)  DNA with proteins bound moves slower in gel electrophoresis. 
E)  More than one of these is important in using DNA for forensic identification.

Question 11

The following blotting procedure is named for the scientist who invented it:

Accepted Answer

A)  Eastern. 
B)  Northern. 
C)  Southern. 
D)  Western. 
E)  More than one of these methods is named for the scientist who invented it. 
A)  Eastern. 
B)  Northern. 
C)  Southern. 
D)  Western. 
E)  More than one of these methods is named for the scientist who invented it.

Question 12

Which of the following was not a technique researchers used to try to improve shelf life of tomatoes with genetic engineering?

Accepted Answer

A)  Insertion of a second polygalactouronase gene 
B)  Using an antisense gene 
C)  Inhibition of the production of ethylene 
D)  Storage in a 40 degree Celcius compartment 
A)  Insertion of a second polygalactouronase gene 
B)  Using an antisense gene 
C)  Inhibition of the production of ethylene 
D)  Storage in a 40 degree Celcius compartment

Question 13

Fluorescent techniques can detect concentrations as low as

Accepted Answer

A)  molar (101 M). 
B)  micromolar (10 − 6 M). 
C)  picomolar (10 − 12 M). 
D)  attamolar (10 − 18 M). 
A)  molar (101 M). 
B)  micromolar (10 − 6 M). 
C)  picomolar (10 − 12 M). 
D)  attamolar (10 − 18 M).

Question 14

​ Which of the following types of polymerase chain reactions (PCR) is used to determine the amount of target DNA initially present in a cell?

Accepted Answer

A)  Nested PCR 
B)  Asymmetric PCR 
C)  Quantitative PCR 
D)  Multiplex PCR 
A)  Nested PCR 
B)  Asymmetric PCR 
C)  Quantitative PCR 
D)  Multiplex PCR

Question 15

Which of the following are methods by which bacteria can be induced to take up recombinant DNA molecules?

Accepted Answer

A)  Heat shock. 
B)  DNA "guns" which spray the DNA at very high speeds. 
C)  Electroporation 
D)  Phage or virus infection. 
E)  All of these are used to transform bacteria. 
A)  Heat shock. 
B)  DNA "guns" which spray the DNA at very high speeds. 
C)  Electroporation 
D)  Phage or virus infection. 
E)  All of these are used to transform bacteria.

Question 16

Which of the following conditions is an example of how genetic engineering is currently used to increase food production?

Accepted Answer

A)  Genetically modified plants have been developed that are resistant to herbicides. 
B)  Genetically modified plants have been developed which contain a gene that confers a natural resistance to certain insects. 
C)  Hormones are given to cows to increase milk production. 
D)  Genetically modified plants have been developed which are resistant to frost. 
E)  All of these are examples of genetic engineering in agriculture. 
A)  Genetically modified plants have been developed that are resistant to herbicides. 
B)  Genetically modified plants have been developed which contain a gene that confers a natural resistance to certain insects. 
C)  Hormones are given to cows to increase milk production. 
D)  Genetically modified plants have been developed which are resistant to frost. 
E)  All of these are examples of genetic engineering in agriculture.

Question 17

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.   Refer to Exhibit 13A. If a recombinant plasmid was obtained inserting DNA into the Bam HI site, screening for the recombinant plasmid can be done by the following technique.

Accepted Answer

A)  Plate on nutrient agar plates that contain ampicillin. 
B)  Plate on nutrient agar plates that contain tetracycline. 
C)  Plate on nutrient agar plates that contain both ampicillin and tetracycline. 
D)  Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline. 
E)  Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin. 
A)  Plate on nutrient agar plates that contain ampicillin. 
B)  Plate on nutrient agar plates that contain tetracycline. 
C)  Plate on nutrient agar plates that contain both ampicillin and tetracycline. 
D)  Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline. 
E)  Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin.

Question 18

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located.   Refer to Exhibit 13A. If a recombinant plasmid were obtained by inserting DNA into the Eco RV site, and the protein corresponding to the recombinant gene were expressed, which of the following statements would be false ?

Accepted Answer

A)  The protein will probably contain some extra amino acids on its N-terminal. 
B)  The plasmid will be resistant to ampicillin. 
C)  The plasmid will not be able to replicate autonomously. 
D)  The protein is not likely to be biologically active without some further treatment. 
E)  The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis. 
A)  The protein will probably contain some extra amino acids on its N-terminal. 
B)  The plasmid will be resistant to ampicillin. 
C)  The plasmid will not be able to replicate autonomously. 
D)  The protein is not likely to be biologically active without some further treatment. 
E)  The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis.

Question 19

While a large majority of people are opposed to somatic gene therapy, a large number support the use of germ-cell gene therapy.

Accepted Answer

A) True 
 B)False

Question 20

Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs. The β -gal gene codes for the enzyme β -galactosidase, the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpR gene confers ampicillin resistance.   Refer to Exhibit 13D. In three separate vessels, the plasmid is treated with the restriction endonucleases Eco RI, Bam HI, and Hind III. Which of the following best represents the electrophoretic gel one would see from these digests?

Accepted Answer

A)    
B)    
C)    
D)    
A)    
B)    
C)    
D)

Exam 13: Nucleic Acid Biotechnology Techniques

If mRNA is converted to DNA, digested with restriction enzymes, and cloned into suitable vectors, the resultant library is called a:

A proteome is

One major obstacle to using bacteria to produce human proteins is because addition of sugar residues to the proteins, glycosylation, never occurs properly.

Which of the following plants have been genetically modified?

When viruses are used as vectors in gene therapy

The RFLP technique

A feature of the Sanger-Coulson method for DNA sequencing is

The best primers for the PCR reaction have the following feature:

Forensic uses of DNA to identify victims or criminals exploit the following trait in DNA:

The following blotting procedure is named for the scientist who invented it:

Which of the following was not a technique researchers used to try to improve shelf life of tomatoes with genetic engineering?

Fluorescent techniques can detect concentrations as low as

​ Which of the following types of polymerase chain reactions (PCR) is used to determine the amount of target DNA initially present in a cell?

Which of the following are methods by which bacteria can be induced to take up recombinant DNA molecules?

Which of the following conditions is an example of how genetic engineering is currently used to increase food production?

Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is located. Refer to Exhibit 13A. If a recombinant plasmid was obtained inserting DNA into the Bam HI site, screening for the recombinant plasmid can be done by the following technique.

While a large majority of people are opposed to somatic gene therapy, a large number support the use of germ-cell gene therapy.

Biochemistry and the Organization of Cells

Water the Solvent for Biochemical Reactions

Amino Acids and Peptides

The Three Dimensional Structure of Proteins

Protein Purification and Characterization Techniques

The Behavior of Proteins Enzymes

The Behavior of Proteins Enzymes Mechanisms and Control

Lipids and Proteins Are Associated in Biological Membranes

Nucleic Acids How Structure Conveys Information

Biosynthesis of Nucleic Acids Replication

Transcription of the Genetic Code the Biosynthesis of Rna

Protein Synthesis Translation of the Genetic Message

Viruses Cancer and Immunology

The Importance of Energy Changes and Electron Transfer in Metabolism

Carbohydrates

Glycolysis

Storage Mechanisms and Control in Carbohydrate Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Lipid Metabolism

Photosynthesis

The Metabolism of Nitrogen

Integration of Metabolism Cellular Signaling

Filters

Which of the following types of polymerase chain reactions (PCR) is used to determine the amount of target DNA initially present in a cell?