Exam 20: Biotechnology

Reproductive cloning of human embryos is generally considered unethical. However, on the subject of therapeutic cloning there is a wider divergence of opinion. Which of the following is a likely explanation?

A researcher is using adult stem cells and comparing them to other adult cells from the same tissue. Which of the following is a likely finding?

Let us suppose that someone is successful at producing induced pluripotent stem cells (iPS)for replacement of pancreatic insulin-producing cells for people with type 1 diabetes. Which of the following could still be problems? I.the possibility that, once introduced into the patient, the iPS cells produce nonpancreatic cells II.the failure of the iPS cells to take up residence in the pancreas III.the inability of the iPS cells to respond to appropriate regulatory signals

In recent times, it has been shown that adult cells can be induced to become pluripotent stem cells (iPS). In order to make this conversion, what has been done to the adult cells?

A principal problem with inserting an unmodified mammalian gene into a BAC, and then getting that gene expressed in bacteria, is that

Use Figure 20.3 to answer the following questions. The DNA profiles that follow represent four different individuals. Figure 20.3 -Which of the following are probably siblings?

The major advantage of using artificial chromosomes such as YACs and BACs for cloning genes is that

One successful form of gene therapy has involved delivery of an allele for the enzyme adenosine deaminase (ADA)to bone marrow cells of a child with SCID, and delivery of these engineered cells back to the bone marrow of the affected child. What is one major reason for the success of this procedure as opposed to many other efforts at gene therapy?

Which of the following is true of embryonic stem cells but not of adult stem cells?

Why are BACs preferred today rather than bacteriophages for making genomic libraries?

Use Figure 20.2 to answer the following question. Figure 20.2 -The segment of DNA shown in Figure 20.2 has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?

Use the following information to answer the next few questions. A group of six students has taken samples of their own cheek cells, purified the DNA, and used a restriction enzyme known to cut at zero, one, or two sites in a particular gene of interest. -Their next two steps, in order, should be