Exam 20: Biotechnology

Use the following information to answer the next few questions. CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes overexpression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early. -Why would Gleevec most probably cause remission of the disease?

Yeast artificial chromosomes contain which of the following elements?

A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?

For a particular microarray assay (DNA chip), cDNA has been made from the mRNAs of a dozen patients' breast tumor biopsies. The researchers will be looking for

RNAi methodology uses double-stranded pieces of RNA to trigger a breakdown or blocking of mRNA. For which of the following might it more possibly be useful?

Which of the following is most closely identical to the formation of twins?

Which of the following techniques used to analyze gene function depends on the specificity of DNA base complementarity?

Why are yeast cells frequently used as hosts for cloning?

The reason for using Taq polymerase for PCR is that

Use Figure 20.3 to answer the following questions. The DNA profiles that follow represent four different individuals. Figure 20.3 -Which of the following statements is consistent with the results?

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of

Which of the following problems with animal cloning might result in premature death of the clones?

DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. What is the purpose of transferring the DNA from a gel to a nitrocellulose paper?

Which of the following is one of the technical reasons why gene therapy is problematic?

Use the following information to answer the next few questions. CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes overexpression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early. -One possible use of transgenic plants is in the production of human proteins, such as vaccines. Which of the following is a possible hindrance that must be overcome?

A student wishes to clone a sequence of DNA of ~200 kb. Which vector would be appropriate?

Genetic engineering is being used by the pharmaceutical industry. Which of the following is not currently one of the uses?

Which of the following modifications is least likely to alter the rate at which a DNA fragment moves through a gel during electrophoresis?

In order to identify a specific restriction fragment using a probe, what must be done?

How does a bacterial cell protect its own DNA from restriction enzymes?