Question 1

Treatment of an individualʹs DNA with restriction endonucleases produces a unique set of DNA fragments. This is the basis of DNA fingerprinting.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following is mismatched?

Accepted Answer

A)  B-DNA: dehydrated 
B)  Z-DNA: left-handed helix 
C)  A-DNA: dehydrated 
D)  Z-DNA: no major or minor grooves 
E)  B-DNA: right-handed helix 
A)  B-DNA: dehydrated 
B)  Z-DNA: left-handed helix 
C)  A-DNA: dehydrated 
D)  Z-DNA: no major or minor grooves 
E)  B-DNA: right-handed helix

Question 3

The abbreviation dGp indicates

Accepted Answer

A)  5ʹ deoxyguanylate. 
B)  3ʹ deoxyguanylate. 
C)  3ʹ, 5ʹ deoxyguanylate. 
D)  5ʹ, 3ʹdeoxyguanylate. 
E)  None of the above 
A)  5ʹ deoxyguanylate. 
B)  3ʹ deoxyguanylate. 
C)  3ʹ, 5ʹ deoxyguanylate. 
D)  5ʹ, 3ʹdeoxyguanylate. 
E)  None of the above

Question 4

Which are the products of the RNase A-catalyzed hydrolysis of pGpApUpApApCpG?

Accepted Answer

A)  pG + pA + pUpA + pA + pCpG 
B)  pGpApU + pApApC + pG 
C)  pG + pApUpApApCpG 
D)  pGpApUp + ApApCp + G 
E)  two pG + three pA + pU + pC 
A)  pG + pA + pUpA + pA + pCpG 
B)  pGpApU + pApApC + pG 
C)  pG + pApUpApApCpG 
D)  pGpApUp + ApApCp + G 
E)  two pG + three pA + pU + pC

Question 5

Absorption of single-stranded DNA at 260 nm is less than that of double-stranded DNA.

Accepted Answer

A) True 
 B)False

Question 6

Alkaline hydrolysis with 0.1 M NaOH is an effective method of degrading RNA and DNA for determining the percent composition of each type of nucleotide monomer.

Accepted Answer

A) True 
 B)False

Question 7

Strands in the stem of stem-loop RNA structures are parallel.

Accepted Answer

A) True 
 B)False

Question 8

ATP is not a nucleoside because it .

Accepted Answer

A)  has phosphate groups 
B)  has three phosphates instead of just one 
C)  lacks the deoxyribosyl group 
D)  is not connect to a carbohydrate group 
A)  has phosphate groups 
B)  has three phosphates instead of just one 
C)  lacks the deoxyribosyl group 
D)  is not connect to a carbohydrate group

Question 9

What is the approximate diameter of a chromatin fiber?

Accepted Answer

A)  3 nm 
B)  30 nm 
C)  300 nm 
D)  3 mm 
E)  30 mm 
A)  3 nm 
B)  30 nm 
C)  300 nm 
D)  3 mm 
E)  30 mm

Question 10

Which enzyme would be least useful for recombination experiments that introduce new fragments of DNA into an existing DNA molecule?

Accepted Answer

A)  EcoRI G↓AATTC 
B)  SmaI CCC↓GGG 
C)  XhoI C↓TCGAG 
D)  All are equally useful 
A)  EcoRI G↓AATTC 
B)  SmaI CCC↓GGG 
C)  XhoI C↓TCGAG 
D)  All are equally useful

Question 11

Which type of RNA carries activated amino acids to the ribosome for assembly into proteins?

Accepted Answer

A)  rRNA 
B)  tRNA 
C)  mRNA 
D)  sRNA 
A)  rRNA 
B)  tRNA 
C)  mRNA 
D)  sRNA

Question 12

Much of the stability of the double-stranded DNA structure is the result of

Accepted Answer

A)  hydrogen bonding between purines. 
B)  the phosphodiester backbone. 
C)  the angle of the planes of the bases with respect to the helix axis. 
D)  the stacking interactions between base pair. 
A)  hydrogen bonding between purines. 
B)  the phosphodiester backbone. 
C)  the angle of the planes of the bases with respect to the helix axis. 
D)  the stacking interactions between base pair.

Question 13

catalyze the hydrolysis of phosphodiester linkages to release nucleotide residues from only one end of a polypeptide chain.

Accepted Answer

A)  Topoisomerases 
B)  Endonucleases 
C)  Exonucleases 
D)  Restriction enzymes 
A)  Topoisomerases 
B)  Endonucleases 
C)  Exonucleases 
D)  Restriction enzymes

Question 14

What is the order of packaging of chromatin in eukaryotic cells from smallest to largest size?

Accepted Answer

A)  double-helix → solenoid → nucleosome → chromosome 
B)  double-helix → chromatin → solenoid → chromosome 
C)  single-stranded DNA → chromatin → histones → chromosome 
D)  double-helix → nucleosomes → solenoid → chromosome 
A)  double-helix → solenoid → nucleosome → chromosome 
B)  double-helix → chromatin → solenoid → chromosome 
C)  single-stranded DNA → chromatin → histones → chromosome 
D)  double-helix → nucleosomes → solenoid → chromosome

Question 15

What is the function of DNA toposiomerases?

Accepted Answer

A)  cleaving nucleotides from the ends of DNA 
B)  adding or removing supercoils 
C)  methylation of the nitrogenous bases 
D)  packaging of DNA into nucleosomes 
A)  cleaving nucleotides from the ends of DNA 
B)  adding or removing supercoils 
C)  methylation of the nitrogenous bases 
D)  packaging of DNA into nucleosomes

Question 16

In proteins, amino acids are linked by peptide bonds; in polynucleotides, nucleotides are linked by

Accepted Answer

A)  phosphoanhydride bonds. 
B)  3ʹ-5ʹphosphodiester bonds. 
C)  5ʹ-3ʹphosphodiester bonds. 
D)  B and C 
E)  All of the above 
A)  phosphoanhydride bonds. 
B)  3ʹ-5ʹphosphodiester bonds. 
C)  5ʹ-3ʹphosphodiester bonds. 
D)  B and C 
E)  All of the above

Question 17

What is a restriction map?

Accepted Answer

A)  the nucleotide sequence recognized by a particular restriction enzyme 
B)  diagram of a DNA molecule to show specific cleavage sites of endonucleases 
C)  table of enzymes that have a least one site in a speciesʹ DNA 
D)  a diagram of the compaction of DNA by packing into nucleosomes and solenoids 
E)  a gel electrophoresis technique used for the purpose of DNA fingerprinting 
A)  the nucleotide sequence recognized by a particular restriction enzyme 
B)  diagram of a DNA molecule to show specific cleavage sites of endonucleases 
C)  table of enzymes that have a least one site in a speciesʹ DNA 
D)  a diagram of the compaction of DNA by packing into nucleosomes and solenoids 
E)  a gel electrophoresis technique used for the purpose of DNA fingerprinting

Question 18

Which statement is not true about histones?

Accepted Answer

A)  They are positively charged at biological pH. 
B)  They only occur in eukaryotes. 
C)  Most species that have histones contain five different types. 
D)  There is wide variation in the amino acid sequences of histones among species. 
A)  They are positively charged at biological pH. 
B)  They only occur in eukaryotes. 
C)  Most species that have histones contain five different types. 
D)  There is wide variation in the amino acid sequences of histones among species.

Question 19

In DNA, phosphodiester bonds form with hydroxyl groups of carbons number and of each deoxyribose sugar.

Accepted Answer

A)  2ʹ; 3ʹ 
B)  2ʹ; 4ʹ 
C)  3ʹ; 4ʹ 
D)  3ʹ; 5ʹ 
E)  4ʹ; 5ʹ 
A)  2ʹ; 3ʹ 
B)  2ʹ; 4ʹ 
C)  3ʹ; 4ʹ 
D)  3ʹ; 5ʹ 
E)  4ʹ; 5ʹ

Question 20

The abbreviation pppT indicates

Accepted Answer

A)  TMP. 
B)  TDP. 
C)  TTP. 
D)  3, 5ʹ TP. 
E)  None of the above 
A)  TMP. 
B)  TDP. 
C)  TTP. 
D)  3, 5ʹ TP. 
E)  None of the above

Exam 19: Nucleic Acids

Treatment of an individualʹs DNA with restriction endonucleases produces a unique set of DNA fragments. This is the basis of DNA fingerprinting.

Which of the following is mismatched?

The abbreviation dGp indicates

Which are the products of the RNase A-catalyzed hydrolysis of pGpApUpApApCpG?

Absorption of single-stranded DNA at 260 nm is less than that of double-stranded DNA.

Alkaline hydrolysis with 0.1 M NaOH is an effective method of degrading RNA and DNA for determining the percent composition of each type of nucleotide monomer.

Strands in the stem of stem-loop RNA structures are parallel.

ATP is not a nucleoside because it .

What is the approximate diameter of a chromatin fiber?

Which enzyme would be least useful for recombination experiments that introduce new fragments of DNA into an existing DNA molecule?

Which type of RNA carries activated amino acids to the ribosome for assembly into proteins?

Much of the stability of the double-stranded DNA structure is the result of

catalyze the hydrolysis of phosphodiester linkages to release nucleotide residues from only one end of a polypeptide chain.

What is the order of packaging of chromatin in eukaryotic cells from smallest to largest size?

What is the function of DNA toposiomerases?

In proteins, amino acids are linked by peptide bonds; in polynucleotides, nucleotides are linked by

What is a restriction map?

Which statement is not true about histones?

In DNA, phosphodiester bonds form with hydroxyl groups of carbons number and of each deoxyribose sugar.

The abbreviation pppT indicates

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Oxidative Phosphorylation

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Dna Replication, Repair, and Recombination

Transcription and Rna Processing

Protein Synthesis

Recombinant Dna Technology

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