Exam 9: Recombinant Dna Technology

Which one of the following analytical techniques does not help illuminate a gene's cellular function?

Which of the following is not needed to build a cDNA library?

A scientist wishes to produce a mammalian protein in E.coli.The protein is a glycoprotein with a molecular weight of 40,000.Approximately 20% of its mass is polysaccharide.The isolated protein is usually phosphorylated and contains three disulfide bonds.The cloned gene contains no introns. (a)What sequences or sites will be required in the vector to get this gene regulated,transcribed,and translated in E.coli? (b)List two problems that might arise in producing a protein identical to that isolated from mammalian cells and describe each problem in no more than two sentences.

A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI,which cuts the plasmid at a single site in the ampicillin-resistance gene.The DNA is then annealed with a PstI digest of human DNA,ligated,and used to transform E.coli cells. (a)What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid? (b)What antibiotic-resistance phenotypes will be found on the plate? (c)Which phenotype will indicate the presence of plasmids that contain human DNA fragments?

The PCR reaction mixture does not include:

What is the essential difference between a genomic library and a cDNA library?