Question 1

Which of the following is not needed in 454 pyrosequencing of DNA?

Accepted Answer

A) dNTPs 
B) Sulfurylase 
C) Luciferase 
D) ddNTPs 
E) Apyrase 
A) dNTPs 
B) Sulfurylase 
C) Luciferase 
D) ddNTPs 
E) Apyrase

Question 2

Which of the following statements about type II restriction enzymes is false?

Accepted Answer

A) Many make staggered (off-center)cuts within their recognition sequences. 
B) Some cut DNA to generate blunt ends. 
C) They are part of a bacterial defense system in which foreign DNA is cleaved. 
D) They cleave and ligate DNA. 
E) They cleave DNA only at recognition sequences specific to a given restriction enzyme. 
A) Many make staggered (off-center)cuts within their recognition sequences. 
B) Some cut DNA to generate blunt ends. 
C) They are part of a bacterial defense system in which foreign DNA is cleaved. 
D) They cleave and ligate DNA. 
E) They cleave DNA only at recognition sequences specific to a given restriction enzyme.

Question 3

A convenient cloning vector with which to introduce foreign DNA into E.coli is a(n):

Accepted Answer

A) 
E) coli chromosome. 
B) messenger RNA. 
C) plasmid. 
D) yeast "ARS" sequence. 
E) yeast transposable element. 
A) 
E) coli chromosome. 
B) messenger RNA. 
C) plasmid. 
D) yeast "ARS" sequence. 
E) yeast transposable element.

Question 4

As the cost of sequencing your personal human genome rapidly approaches $1,000,consider the benefits and risks of having every fetus' DNA sequenced before birth.

Accepted Answer

Answers are expected to vary,but key poi

Question 5

Which would you expect to be larger,the percentage of the human genome that is translated into protein or the percentage of the genome of a bacterium that is translated into protein? Why?

Accepted Answer

Because bacteria do not have i

Question 6

Restriction enzymes:

Accepted Answer

A) act at the membrane to restrict the passage of certain molecules into the cell. 
B) are highly specialized ribonucleases that degrade mRNA soon after its synthesis. 
C) are sequence-specific DNA endonucleases. 
D) are very specific proteases that cleave peptides at only certain sequences. 
E) catalyze the addition of a certain amino acid to a specific tRNA. 
A) act at the membrane to restrict the passage of certain molecules into the cell. 
B) are highly specialized ribonucleases that degrade mRNA soon after its synthesis. 
C) are sequence-specific DNA endonucleases. 
D) are very specific proteases that cleave peptides at only certain sequences. 
E) catalyze the addition of a certain amino acid to a specific tRNA.

Question 7

When bacterial artificial chromosomes (BACs)are used as cloning vectors,what size of DNA fragment can be cloned?

Accepted Answer

100,000 to

Question 8

Which type of DNA sequence is not found in the human genome?

Accepted Answer

A) Long repetitive repeats 
B) Introns 
C) Retro-palindromes 
D) Simple sequence repeats 
E) Transposons 
A) Long repetitive repeats 
B) Introns 
C) Retro-palindromes 
D) Simple sequence repeats 
E) Transposons

Question 9

What is a DNA microarray? How does it resemble and how does it differ from a DNA library?

Accepted Answer

A DNA microarray is a solid surface upon

Question 10

Explain briefly the properties of the plasmid pBR322 that make it so convenient as a vector for cloning fragments of foreign DNA.

Accepted Answer

pBR322 has two antibiotic resistance mar

Question 11

Rank the following organisms in order from smallest genome (number of base pairs of DNA)to largest genome.

Accepted Answer

A) Human,fruit fly,E.coli bacterium 
B) 
E) coli bacterium,human,fruit fly 
C) 
E) coli bacterium,fruit fly,human 
D) Fruit fly,E.coli bacterium,human 
E) Fruit fly,human,E.coli bacterium 
A) Human,fruit fly,E.coli bacterium 
B) 
E) coli bacterium,human,fruit fly 
C) 
E) coli bacterium,fruit fly,human 
D) Fruit fly,E.coli bacterium,human 
E) Fruit fly,human,E.coli bacterium

Question 12

Distinguish between protein function at the molecular,cellular,and phenotypic level.

Accepted Answer

Molecular function describes the precise

Question 13

Which of the following is not a commonly used tag for affinity purification of cloned proteins?

Accepted Answer

A) Glutathione-S-transferase 
B) Maltose binding protein 
C) Nickel 
D) Protein A 
E) Chitin-binding domain 
A) Glutathione-S-transferase 
B) Maltose binding protein 
C) Nickel 
D) Protein A 
E) Chitin-binding domain

Question 14

Explain the importance of outgroups for understanding genomic lineages.

Accepted Answer

This question is best answered by consid

Question 15

Explain why a probe designed to detect a gene encoding a particular amino acid sequence must usually consist of a mixture of different DNA sequences rather than only one sequence.

Accepted Answer

Since the genetic code is degenerate—mos

Question 16

Which of the following does not apply to the construction or use of a DNA library?

Accepted Answer

A) Determining the location of a particular DNA sequence in a DNA library requires a suitable hybridization probe. 
B) Genomic libraries are better for expressing gene products than cDNA libraries. 
C) Many segments of DNA from a cellular genome are cloned. 
D) Specialized DNA libraries can be made by cloning DNA copies of mRNAs. 
E) The DNA copies of mRNA found in a cDNA library are made by reverse transcriptase. 
A) Determining the location of a particular DNA sequence in a DNA library requires a suitable hybridization probe. 
B) Genomic libraries are better for expressing gene products than cDNA libraries. 
C) Many segments of DNA from a cellular genome are cloned. 
D) Specialized DNA libraries can be made by cloning DNA copies of mRNAs. 
E) The DNA copies of mRNA found in a cDNA library are made by reverse transcriptase.

Question 17

In the laboratory,recombinant plasmids are commonly introduced into bacterial cells by:

Accepted Answer

A) electrophoresis-a gentle low-voltage gradient draws the DNA into the cell. 
B) infection with a bacteriophage that carries the plasmid. 
C) microinjection. 
D) mixing plasmids with an extract of broken cells. 
E) transformation-heat shock of the cells incubated with plasmid DNA in the presence of CaCl2. 
A) electrophoresis-a gentle low-voltage gradient draws the DNA into the cell. 
B) infection with a bacteriophage that carries the plasmid. 
C) microinjection. 
D) mixing plasmids with an extract of broken cells. 
E) transformation-heat shock of the cells incubated with plasmid DNA in the presence of CaCl2.

Question 18

What happens in PCR if you use DNA polymerase derived from E.coli instead of from a thermostable source?

Accepted Answer

In the very first denaturation

Question 19

Which of the following is not used as a heterologous host for the expression of recombinant proteins?

Accepted Answer

A) Retroviruses 
B) Bacteria such as E.coli 
C) Eukaryotes such as S.cerevisiae 
D) Insect cells 
E) Mammalian cells 
A) Retroviruses 
B) Bacteria such as E.coli 
C) Eukaryotes such as S.cerevisiae 
D) Insect cells 
E) Mammalian cells

Question 20

How does a bacterial artificial chromosome (BAC)differ from a plasmid?

Accepted Answer

A BAC is a plasmid that has a very stabl

Exam 9: Recombinant Dna Technology

Which of the following is not needed in 454 pyrosequencing of DNA?

Which of the following statements about type II restriction enzymes is false?

A convenient cloning vector with which to introduce foreign DNA into E.coli is a(n):

As the cost of sequencing your personal human genome rapidly approaches $1,000,consider the benefits and risks of having every fetus' DNA sequenced before birth.

Which would you expect to be larger,the percentage of the human genome that is translated into protein or the percentage of the genome of a bacterium that is translated into protein? Why?

Restriction enzymes:

When bacterial artificial chromosomes (BACs)are used as cloning vectors,what size of DNA fragment can be cloned?

Which type of DNA sequence is not found in the human genome?

What is a DNA microarray? How does it resemble and how does it differ from a DNA library?

Explain briefly the properties of the plasmid pBR322 that make it so convenient as a vector for cloning fragments of foreign DNA.

Rank the following organisms in order from smallest genome (number of base pairs of DNA)to largest genome.

Distinguish between protein function at the molecular,cellular,and phenotypic level.

Which of the following is not a commonly used tag for affinity purification of cloned proteins?

Explain the importance of outgroups for understanding genomic lineages.

Explain why a probe designed to detect a gene encoding a particular amino acid sequence must usually consist of a mixture of different DNA sequences rather than only one sequence.

Which of the following does not apply to the construction or use of a DNA library?

In the laboratory,recombinant plasmids are commonly introduced into bacterial cells by:

What happens in PCR if you use DNA polymerase derived from E.coli instead of from a thermostable source?

Which of the following is not used as a heterologous host for the expression of recombinant proteins?

How does a bacterial artificial chromosome (BAC)differ from a plasmid?

The Foundations of Biochemistry

Water

Amino Acids, peptides, and Proteins

The Three-Dimensional Structure of Proteins

Protein Function

Enzymes

Carbohydrates and Glycobiology

Nucleotides and Nucleic Acids

Lipids

Biological Membranes and Transport

Biosignaling

Principles of Bioenergetics

Glycolysis, gluconeogenesis, and the Pentose Phosphate Pathway

Principles of Metabolic Regulation

The Citric Acid Cycle

Fatty Acid Catabolism

Amino Acid Oxidation and the Production of Urea

Oxidative Phosphorylation and Photophosphorylation

Carbohydrate Biosynthesis in Plants and Bacteria

Lipid Biosynthesis

Biosynthesis of Amino Acids, nucleotides, and Related Molecules

Integration and Hormonal Regulation of Mammalian Metabolism

Genes and Chromosomes

Dna Metabolism

Rna Metabolism

Protein Metabolism

Regulation of Gene Expression

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