Question 1

What is the major difference between green fluorescent protein and an antibiotic resistance gene in terms of their usefulness as a reporter gene?

Accepted Answer

A)  The green fluorescent protein can be used only when the host cell is a plant cell because it relies on the process of photosynthesis. 
B)  The antibiotic resistance gene can be used only when the host cell is an animal cell. 
C)  The green fluorescent protein can be used only in transfection,not in transformation. 
D)  With the green fluorescent protein,no cells are killed in the selection process. 
E)  Homologous recombination will work only with the green fluorescent protein. 
A)  The green fluorescent protein can be used only when the host cell is a plant cell because it relies on the process of photosynthesis. 
B)  The antibiotic resistance gene can be used only when the host cell is an animal cell. 
C)  The green fluorescent protein can be used only in transfection,not in transformation. 
D)  With the green fluorescent protein,no cells are killed in the selection process. 
E)  Homologous recombination will work only with the green fluorescent protein.

Question 2

Which of the following would be most appropriate for inhibiting transcription of a specific gene?

Accepted Answer

A)  Antisense RNA 
B)  RNA interference 
C)  DNA chips 
D)  Homologous recombination 
E)  PCR 
A)  Antisense RNA 
B)  RNA interference 
C)  DNA chips 
D)  Homologous recombination 
E)  PCR

Question 3

Which characteristic makes plasmids useful as a vector?

Accepted Answer

A)  Many plasmids contain genes that convey antibiotic resistance. 
B)  Unlike viruses,plasmids do not need to be coaxed by artificial means to infect cells. 
C)  Plasmids can accommodate a large amount (over 100 kb)of DNA. 
D)  Plasmids use the same origin of replication as eukaryotic cells. 
E)  A cell can only host one plasmid at a time. 
A)  Many plasmids contain genes that convey antibiotic resistance. 
B)  Unlike viruses,plasmids do not need to be coaxed by artificial means to infect cells. 
C)  Plasmids can accommodate a large amount (over 100 kb)of DNA. 
D)  Plasmids use the same origin of replication as eukaryotic cells. 
E)  A cell can only host one plasmid at a time.

Question 4

Most restriction sites are _______ base pairs long.

Accepted Answer

A)  1 or 2 
B)  4 to 6 
C)  about 10 
D)  about 20 
E)  over 50 
A)  1 or 2 
B)  4 to 6 
C)  about 10 
D)  about 20 
E)  over 50

Question 5

The use of _______ allows researchers to examine the patterns of gene expression for a large number of genes simultaneously.

Accepted Answer

A)  antisense RNA 
B)  RNAi 
C)  DNA microarray 
D)  PCR 
E)  homologous recombination 
A)  antisense RNA 
B)  RNAi 
C)  DNA microarray 
D)  PCR 
E)  homologous recombination

Question 6

Transgenic cells are created via the _______ of recombinant DNA into cells.

Accepted Answer

A)  recombination 
B)  transfection 
C)  ligation 
D)  restriction 
E)  complementation 
A)  recombination 
B)  transfection 
C)  ligation 
D)  restriction 
E)  complementation

Question 7

Which research technique necessarily makes use of reverse transcriptase?

Accepted Answer

A)  Gene cloning 
B)  Construction of a gene library 
C)  Construction of a cDNA library 
D)  Gene knockout studies 
E)  Pharming 
A)  Gene cloning 
B)  Construction of a gene library 
C)  Construction of a cDNA library 
D)  Gene knockout studies 
E)  Pharming

Question 8

The crew of an eighteenth-century ship sailing from Asia has eaten mostly rice during the voyage.Some of the crew members start experiencing vision difficulties and an increased susceptibility to infection.The most likely cause of these symptoms is a deficiency in

Accepted Answer

A)  vitamin B12. 
B)  vitamin C. 
C)  $\beta$-carotene. 
D)  glyphosate. 
E)  oblimersen. 
A)  vitamin B12. 
B)  vitamin C. 
C)  $\beta$-carotene. 
D)  glyphosate. 
E)  oblimersen.

Question 9

The DNA from the genome of an organism is chopped into numerous fragments,which then are inserted into vectors that are taken up by host cells.The genetic information that is present in the resulting colonies is called a genomic

Accepted Answer

A)  collection. 
B)  library. 
C)  museum. 
D)  bank. 
E)  farm. 
A)  collection. 
B)  library. 
C)  museum. 
D)  bank. 
E)  farm.

Question 10

Which characteristic is a limitation of conventional breeding,as compared with recombinant DNA technology?

Accepted Answer

A)  The limited range of useful genetic variation 
B)  The length of time required by the conventional breeding process 
C)  The lack of precision 
D)  The instability of desirable traits 
E)  All of the above 
A)  The limited range of useful genetic variation 
B)  The length of time required by the conventional breeding process 
C)  The lack of precision 
D)  The instability of desirable traits 
E)  All of the above

Question 11

Which process is used by researchers to inhibit translation of specific genes?

Accepted Answer

A)  Antisense RNA 
B)  Targeted mutation 
C)  DNA microarrays 
D)  Homologous recombination 
E)  Transformation 
A)  Antisense RNA 
B)  Targeted mutation 
C)  DNA microarrays 
D)  Homologous recombination 
E)  Transformation

Question 12

Which statement about RNAi technology is false?

Accepted Answer

A)  It is based on a natural process for inhibiting translation. 
B)  It was discovered only recently (in the 1990s). 
C)  It has been used as a therapy for macular degeneration. 
D)  It involves both single-stranded and double-stranded RNA. 
E)  It can be used to block gene expression only in cell lines,not in living organisms. 
A)  It is based on a natural process for inhibiting translation. 
B)  It was discovered only recently (in the 1990s). 
C)  It has been used as a therapy for macular degeneration. 
D)  It involves both single-stranded and double-stranded RNA. 
E)  It can be used to block gene expression only in cell lines,not in living organisms.

Question 13

The use of biotechnological approaches for the improvement of crop plants has been more controversial than their use to prepare medically useful products.Why?

Accepted Answer

A)  People eat food that could contain transgenes. 
B)  Crops are grown outside,and there is a chance that a transgene could escape to other organisms. 
C)  Herbicide resistance could spread to weed species. 
D)  Beneficial insects could be harmed by plants expressing the Bt toxin. 
E)  All of the above 
A)  People eat food that could contain transgenes. 
B)  Crops are grown outside,and there is a chance that a transgene could escape to other organisms. 
C)  Herbicide resistance could spread to weed species. 
D)  Beneficial insects could be harmed by plants expressing the Bt toxin. 
E)  All of the above

Question 14

DNA is _______ charged due to the presence of a _______ group.

Accepted Answer

A)  negatively;phosphate 
B)  negatively;methyl 
C)  negatively;carbon 
D)  positively;methyl 
E)  positively;phosphate 
A)  negatively;phosphate 
B)  negatively;methyl 
C)  negatively;carbon 
D)  positively;methyl 
E)  positively;phosphate

Question 15

Which statement about human growth hormone is false?

Accepted Answer

A)  Its production in transgenic animals is a good example of pharming. 
B)  The supply obtained from dead people is inadequate to meet the demand. 
C)  It is produced by the pituitary gland. 
D)  It is a long fatty acid chain. 
E)  It cannot be produced using pharming. 
A)  Its production in transgenic animals is a good example of pharming. 
B)  The supply obtained from dead people is inadequate to meet the demand. 
C)  It is produced by the pituitary gland. 
D)  It is a long fatty acid chain. 
E)  It cannot be produced using pharming.

Question 16

Recombinant DNA technology would be least applicable to which approach?

Accepted Answer

A)  Analysis of traits determined by multiple genes 
B)  Overexpressing a particular gene 
C)  Silencing a particular gene 
D)  Knocking out a particular gene 
E)  Targeting a protein to the nucleus 
A)  Analysis of traits determined by multiple genes 
B)  Overexpressing a particular gene 
C)  Silencing a particular gene 
D)  Knocking out a particular gene 
E)  Targeting a protein to the nucleus

Question 17

Bacteria protect themselves from their own restriction enzymes by

Accepted Answer

A)  means of their blunt ends. 
B)  means of their sticky ends. 
C)  adding methyl groups at restriction sites. 
D)  means of DNA ligase. 
E)  transforming themselves. 
A)  means of their blunt ends. 
B)  means of their sticky ends. 
C)  adding methyl groups at restriction sites. 
D)  means of DNA ligase. 
E)  transforming themselves.

Question 18

In general,which genetically altered food would most likely be of concern regarding human health?

Accepted Answer

A)  Food in which the B.thuringiensis toxin has been added 
B)  Food that contains an inserted gene that allows plants to grow better in high salt conditions 
C)  Food that contains an inserted gene that allows plants to grow faster 
D)  Food that contains an inserted gene that produces $\beta$-carotene 
E)  Food that contains an inserted gene that increases crop yield 
A)  Food in which the B.thuringiensis toxin has been added 
B)  Food that contains an inserted gene that allows plants to grow better in high salt conditions 
C)  Food that contains an inserted gene that allows plants to grow faster 
D)  Food that contains an inserted gene that produces $\beta$-carotene 
E)  Food that contains an inserted gene that increases crop yield

Question 19

Which sequence forms a palindromic sequence in a DNA molecule?

Accepted Answer

A)  GTCCCG 
B)  GTACGC 
C)  TACG 
D)  TACT 
E)  ACGT 
A)  GTCCCG 
B)  GTACGC 
C)  TACG 
D)  TACT 
E)  ACGT

Question 20

Use the following to answer questions :
A plasmid has genes that confer resistance to both ampicillin and tetracycline.Foreign DNA is inserted at a restriction enzyme recognition site that lies within the ampicillin gene.
-After host cells have been given the opportunity to take up the plasmid,which type of cells should be the rarest?

Accepted Answer

A)  Bacteria with no plasmid and no foreign DNA 
B)  Bacteria with the foreign DNA but no plasmid 
C)  Bacteria with the recombinant plasmid 
D)  Bacteria with the nonrecombinant plasmid 
E)  All types should appear with approximately the same frequency. 
A)  Bacteria with no plasmid and no foreign DNA 
B)  Bacteria with the foreign DNA but no plasmid 
C)  Bacteria with the recombinant plasmid 
D)  Bacteria with the nonrecombinant plasmid 
E)  All types should appear with approximately the same frequency.

Exam 13: Biotechnology

What is the major difference between green fluorescent protein and an antibiotic resistance gene in terms of their usefulness as a reporter gene?

Which of the following would be most appropriate for inhibiting transcription of a specific gene?

Which characteristic makes plasmids useful as a vector?

Most restriction sites are _______ base pairs long.

The use of _______ allows researchers to examine the patterns of gene expression for a large number of genes simultaneously.

Transgenic cells are created via the _______ of recombinant DNA into cells.

Which research technique necessarily makes use of reverse transcriptase?

The crew of an eighteenth-century ship sailing from Asia has eaten mostly rice during the voyage.Some of the crew members start experiencing vision difficulties and an increased susceptibility to infection.The most likely cause of these symptoms is a deficiency in

The DNA from the genome of an organism is chopped into numerous fragments,which then are inserted into vectors that are taken up by host cells.The genetic information that is present in the resulting colonies is called a genomic

Which characteristic is a limitation of conventional breeding,as compared with recombinant DNA technology?

Which process is used by researchers to inhibit translation of specific genes?

Which statement about RNAi technology is false?

The use of biotechnological approaches for the improvement of crop plants has been more controversial than their use to prepare medically useful products.Why?

DNA is _______ charged due to the presence of a _______ group.

Which statement about human growth hormone is false?

Recombinant DNA technology would be least applicable to which approach?

Bacteria protect themselves from their own restriction enzymes by

In general,which genetically altered food would most likely be of concern regarding human health?

Which sequence forms a palindromic sequence in a DNA molecule?

Introduction

The Chemistry and Energy of Life

Nucleic Acids, Proteins, and Enzymes

Cells: the Working Units of Life

Cell Membranes and Signaling

Pathways That Harvest and Store Chemical Energy

The Cell Cycle and Cell Division

Inheritance, Genes, and Chromosomes

DNA and Its Role in Heredity

From Dna to Protein: Gene Expression

Regulation of Gene Expression

Genomes

Genes, Development, and Evolution

Processes of Evolution

Reconstructing and Using Phylogenies

Speciation

The History of Life on Earth

Bacteria, Archaea, and Viruses

The Origin and Diversification of Eukaryotes

The Evolution of Plants

The Evolution and Diversity of Fungi

Animal Origins and Diversity

The Plant Body

Plant Nutrition and Transport

Plant Growth and Development

Reproduction of Flowering Plants

Plants in the Environment

Fundamentals of Animal Function

Nutrition and Digestion

Breathing

Circulation

Muscle and Movement

Neurons, Sense Organs, and Nervous Systems

Nervous and Endocrine Control

Water and Salts: Excretion

Animal Reproduction

Animal Development

Immunology: Animal Defense Systems

Animal Behavior

The Distribution of Earths Ecological Systems

Populations

Ecological and Evolutionary Consequences of Interactions Within and Among Species

Ecological Communities

The Global Ecosystem

Filters

DNA is _ charged due to the presence of a _ group.