Question 1

In gel electrophoresis,one can determine the relative abundance of a fragment by

Accepted Answer

A)  examining how quickly it moves on the gel;the fastest moving fragments are the most abundant. 
B)  examining how quickly it moves on the gel;the slowest moving fragments are the most abundant. 
C)  examining the intensity of the band. 
D)  using DNA ligase. 
E)  using a selectable marker. 
A)  examining how quickly it moves on the gel;the fastest moving fragments are the most abundant. 
B)  examining how quickly it moves on the gel;the slowest moving fragments are the most abundant. 
C)  examining the intensity of the band. 
D)  using DNA ligase. 
E)  using a selectable marker.

Question 2

Suppose the first three nucleotides of a restriction site are AGT.If the restriction site is six nucleotides long,its next three nucleotides will be _______.

Accepted Answer

The answer of Suppose the first three nucleotides of a...

Question 3

Which characteristic would not be useful in a vector?

Accepted Answer

A)  Multiple unique restriction enzyme recognition sequences 
B)  Antibiotic resistant gene 
C)  The ability to replicate independently inside the host cell 
D)  Visual reporter gene 
E)  Large size relative to the host chromosomes 
A)  Multiple unique restriction enzyme recognition sequences 
B)  Antibiotic resistant gene 
C)  The ability to replicate independently inside the host cell 
D)  Visual reporter gene 
E)  Large size relative to the host chromosomes

Question 4

An important vector for the manipulation of plant genes comes from the bacterium A.tumefaciens and is called

Accepted Answer

A)  an EcoRI plasmid. 
B)  $\lambda$ bacteriophage. 
C)  BamHI. 
D)  Ti plasmid. 
E)  a LacZ plasmid. 
A)  an EcoRI plasmid. 
B)  $\lambda$ bacteriophage. 
C)  BamHI. 
D)  Ti plasmid. 
E)  a LacZ plasmid.

Question 5

Why are plant cells particularly useful as hosts for recombinant DNA?

Accepted Answer

A)  Animal cells cannot be cultured. 
B)  Plant cells lack introns. 
C)  Plant cells can be dedifferentiated into stem cells. 
D)  Plant cells have reverse transcriptase. 
E)  Plant cells have plasmids. 
A)  Animal cells cannot be cultured. 
B)  Plant cells lack introns. 
C)  Plant cells can be dedifferentiated into stem cells. 
D)  Plant cells have reverse transcriptase. 
E)  Plant cells have plasmids.

Question 6

DNA is cut at specific sites by means of

Accepted Answer

A)  DNA ligases. 
B)  transposons. 
C)  restriction enzymes. 
D)  DNA silicases. 
E)  DNA methylases. 
A)  DNA ligases. 
B)  transposons. 
C)  restriction enzymes. 
D)  DNA silicases. 
E)  DNA methylases.

Question 7

What is the major limitation of the dried preparation of the insecticide Bt that is produced by Bacillus thuringiensis?

Accepted Answer

A)  It has not been approved for use in the United States. 
B)  It degrades so quickly that it has to be applied repeatedly. 
C)  It is not very toxic. 
D)  It is new and untested. 
E)  It is difficult to produce. 
A)  It has not been approved for use in the United States. 
B)  It degrades so quickly that it has to be applied repeatedly. 
C)  It is not very toxic. 
D)  It is new and untested. 
E)  It is difficult to produce.

Question 8

Which enzyme would be used to join two DNA fragments?

Accepted Answer

A)  Reverse transcriptase 
B)  DNA polymerase 
C)  DNA recombinase 
D)  DNA endonuclease 
E)  DNA ligase 
A)  Reverse transcriptase 
B)  DNA polymerase 
C)  DNA recombinase 
D)  DNA endonuclease 
E)  DNA ligase

Question 9

Which tool used in biotechnology is derived from an evolutionary adaptation of certain bacteria?

Accepted Answer

A)  Restriction enzymes 
B)  Gel electrophoresis 
C)  Phage vectors 
D)  DNA cloning 
E)  Infection 
A)  Restriction enzymes 
B)  Gel electrophoresis 
C)  Phage vectors 
D)  DNA cloning 
E)  Infection

Question 10

Which statement about insulin is false?

Accepted Answer

A)  It is a polypeptide and a hormone. 
B)  Human and cattle insulin differ in terms of their amino acid sequence. 
C)  People given cattle insulin can develop allergic reactions to it. 
D)  It is easy to harvest insulin from living people. 
E)  Insulin is made up of two polypeptide chains. 
A)  It is a polypeptide and a hormone. 
B)  Human and cattle insulin differ in terms of their amino acid sequence. 
C)  People given cattle insulin can develop allergic reactions to it. 
D)  It is easy to harvest insulin from living people. 
E)  Insulin is made up of two polypeptide chains.

Question 11

In the construction of a transgenic cell,a gene that enables growth on arabinose is added to the vector.This gene is being used as a

Accepted Answer

A)  reporter gene. 
B)  replicon. 
C)  cloning vector. 
D)  blunt end. 
E)  expression vector. 
A)  reporter gene. 
B)  replicon. 
C)  cloning vector. 
D)  blunt end. 
E)  expression vector.

Question 12

Which characteristic is not one of the properties of yeast cells that have made them useful eukaryotic hosts for recombinant DNA studies?

Accepted Answer

A)  Their rapid rate of cell division 
B)  Their small genome size 
C)  Their ease of growth in the laboratory 
D)  Their ability to ferment alcohols 
E)  Their similarity to most other eukaryotic cells 
A)  Their rapid rate of cell division 
B)  Their small genome size 
C)  Their ease of growth in the laboratory 
D)  Their ability to ferment alcohols 
E)  Their similarity to most other eukaryotic cells

Question 13

The synthesis of oligonucleotides is most directly useful for

Accepted Answer

A)  homologous recombination. 
B)  transformation. 
C)  the polymerase chain reaction. 
D)  electroporation. 
E)  electrophoresis. 
A)  homologous recombination. 
B)  transformation. 
C)  the polymerase chain reaction. 
D)  electroporation. 
E)  electrophoresis.

Question 14

Which statement about mouse embryonic stem cells is false?

Accepted Answer

A)  They can serve as hosts for transfection studies using homologous recombination. 
B)  They are unspecialized. 
C)  They can be used in knockout studies. 
D)  They can be transplanted into a blastocyst. 
E)  They never differentiate into specialized cells. 
A)  They can serve as hosts for transfection studies using homologous recombination. 
B)  They are unspecialized. 
C)  They can be used in knockout studies. 
D)  They can be transplanted into a blastocyst. 
E)  They never differentiate into specialized cells.

Question 15

Which process is not an example of biotechnology?

Accepted Answer

A)  Wine making 
B)  Selective breeding of cattle to increase milk yield 
C)  Introduction of a plasmid vector into a plant 
D)  Creation of a synthetic fiber 
E)  Use of yeast to brew beer and wine 
A)  Wine making 
B)  Selective breeding of cattle to increase milk yield 
C)  Introduction of a plasmid vector into a plant 
D)  Creation of a synthetic fiber 
E)  Use of yeast to brew beer and wine

Question 16

What information would be needed to design a synthetic gene for a protein to be translated in yeast and used medically?

Accepted Answer

A)  The primary structure of the protein and the genetic code 
B)  The primary and secondary structures of the protein and the genetic code 
C)  The primary structure of the protein,the genetic code,and the yeast promoter sequence 
D)  The secondary structure of the protein,the genetic code,and the yeast promoter sequence 
E)  The primary structure of the protein and the yeast promoter sequence 
A)  The primary structure of the protein and the genetic code 
B)  The primary and secondary structures of the protein and the genetic code 
C)  The primary structure of the protein,the genetic code,and the yeast promoter sequence 
D)  The secondary structure of the protein,the genetic code,and the yeast promoter sequence 
E)  The primary structure of the protein and the yeast promoter sequence

Question 17

Which statement about restriction enzymes is true?

Accepted Answer

A)  They cut at regular intervals,every 4,000 base pairs. 
B)  They prefer to cut at DNA that has been methylated. 
C)  Restriction sites are palindromic sequences. 
D)  They only cut eukaryotic DNA. 
E)  An active restriction enzyme consists of one subunit. 
A)  They cut at regular intervals,every 4,000 base pairs. 
B)  They prefer to cut at DNA that has been methylated. 
C)  Restriction sites are palindromic sequences. 
D)  They only cut eukaryotic DNA. 
E)  An active restriction enzyme consists of one subunit.

Question 18

EcoRI makes staggered cuts when it cleaves DNA,creating single-stranded tails called "sticky ends." These ends can form a complementary base pair.Assuming that the concentration of cut DNA strands is not rate limiting in order for the complementary ends to base pair,which condition is necessary?

Accepted Answer

A)  The presence of specific helicases 
B)  Sufficiently high temperature to dissociate complementary strands 
C)  Methyl groups at the end of each DNA strand 
D)  Sufficiently low temperature to encourage hydrogen binding of DNA strands 
E)  Phosphodiester bonds between the complimentary DNA strands 
A)  The presence of specific helicases 
B)  Sufficiently high temperature to dissociate complementary strands 
C)  Methyl groups at the end of each DNA strand 
D)  Sufficiently low temperature to encourage hydrogen binding of DNA strands 
E)  Phosphodiester bonds between the complimentary DNA strands

Question 19

Which statement about recombinant DNA is false?

Accepted Answer

A)  Restriction enzymes can create DNA fragments in such a way as to leave sticky ends. 
B)  A single restriction enzyme can cut DNA sequences at several different recognition sites. 
C)  Restriction enzymes cut DNA at palindromic sequences. 
D)  Restriction enzymes can create DNA fragments with blunt ends. 
E)  Complementary sticky ends can form hydrogen bonds with each other. 
A)  Restriction enzymes can create DNA fragments in such a way as to leave sticky ends. 
B)  A single restriction enzyme can cut DNA sequences at several different recognition sites. 
C)  Restriction enzymes cut DNA at palindromic sequences. 
D)  Restriction enzymes can create DNA fragments with blunt ends. 
E)  Complementary sticky ends can form hydrogen bonds with each other.

Question 20

The production of many copies of a particular gene is called

Accepted Answer

A)  transfection. 
B)  restriction. 
C)  cloning. 
D)  annealing. 
E)  inducing. 
A)  transfection. 
B)  restriction. 
C)  cloning. 
D)  annealing. 
E)  inducing.

Exam 13: Biotechnology

In gel electrophoresis,one can determine the relative abundance of a fragment by

Suppose the first three nucleotides of a restriction site are AGT.If the restriction site is six nucleotides long,its next three nucleotides will be _______.

Which characteristic would not be useful in a vector?

An important vector for the manipulation of plant genes comes from the bacterium A.tumefaciens and is called

Why are plant cells particularly useful as hosts for recombinant DNA?

DNA is cut at specific sites by means of

What is the major limitation of the dried preparation of the insecticide Bt that is produced by Bacillus thuringiensis?

Which enzyme would be used to join two DNA fragments?

Which tool used in biotechnology is derived from an evolutionary adaptation of certain bacteria?

Which statement about insulin is false?

In the construction of a transgenic cell,a gene that enables growth on arabinose is added to the vector.This gene is being used as a

Which characteristic is not one of the properties of yeast cells that have made them useful eukaryotic hosts for recombinant DNA studies?

The synthesis of oligonucleotides is most directly useful for

Which statement about mouse embryonic stem cells is false?

Which process is not an example of biotechnology?

What information would be needed to design a synthetic gene for a protein to be translated in yeast and used medically?

Which statement about restriction enzymes is true?

EcoRI makes staggered cuts when it cleaves DNA,creating single-stranded tails called "sticky ends." These ends can form a complementary base pair.Assuming that the concentration of cut DNA strands is not rate limiting in order for the complementary ends to base pair,which condition is necessary?

Which statement about recombinant DNA is false?

The production of many copies of a particular gene is called

Introduction

The Chemistry and Energy of Life

Nucleic Acids, Proteins, and Enzymes

Cells: the Working Units of Life

Cell Membranes and Signaling

Pathways That Harvest and Store Chemical Energy

The Cell Cycle and Cell Division

Inheritance, Genes, and Chromosomes

DNA and Its Role in Heredity

From Dna to Protein: Gene Expression

Regulation of Gene Expression

Genomes

Genes, Development, and Evolution

Processes of Evolution

Reconstructing and Using Phylogenies

Speciation

The History of Life on Earth

Bacteria, Archaea, and Viruses

The Origin and Diversification of Eukaryotes

The Evolution of Plants

The Evolution and Diversity of Fungi

Animal Origins and Diversity

The Plant Body

Plant Nutrition and Transport

Plant Growth and Development

Reproduction of Flowering Plants

Plants in the Environment

Fundamentals of Animal Function

Nutrition and Digestion

Breathing

Circulation

Muscle and Movement

Neurons, Sense Organs, and Nervous Systems

Nervous and Endocrine Control

Water and Salts: Excretion

Animal Reproduction

Animal Development

Immunology: Animal Defense Systems

Animal Behavior

The Distribution of Earths Ecological Systems

Populations

Ecological and Evolutionary Consequences of Interactions Within and Among Species

Ecological Communities

The Global Ecosystem

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