Exam 13: Biotechnology

Variation at a specific gene is known to affect metabolism in mice,but it is not clear which of the nucleotides are responsible for the changes.In addition to recombinant DNA technology,which technique would be most relevant for revealing which of the nucleotides affect metabolism?

The enzyme that catalyzes the joining of DNA fragments is known as _______.

The primary benefit of using genetic markers in plant breeding is that this technology

Which sequence could hypothetically form a restriction enzyme recognition site in DNA?

Which statement about microarrays is false?

What does the "c" in "cDNA library" stand for?

Which statement about recombinant DNA biotechnology is false?

An engineered DNA molecule that contains sequences from cattle and sequences from bacteria would be considered to be _______ DNA.

A plasmid genomic library and a phage genomic library are constructed from the same genetic material.Which statement is true?

Why is the use of siRNA preferred to miRNA as a means for blocking translation of a specific mRNA?

Which statement about biotechnology is false?

Use the following to answer questions : Suppose one restriction enzyme (enzyme A)makes two cuts in a linear DNA fragment and another restriction enzyme (enzyme B)makes one cut in that same DNA.The cuts are not in the same place,and none of the cut fragments are exactly the same size.The restriction digest is set up on an electrophoresis gel. -How many bands should the gel pick up if only enzyme A is used in the restriction digest?

A restriction enzyme cuts a 25,700 bp segment of DNA into three fragments.Fragment A is 5,800 bp and fragment B is 6,600 bp.How long is fragment C,and which of the fragments will move the farthest in an electrophoresis gel?

Which DNA sequence would most likely be recognized by a restriction enzyme?

Which process would make use of homologous recombination?

When restriction enzymes make cuts with _______ ends,the exposed DNA sequences can form _______ bonds with complementary sequences.

The field of industrial biotechnology was launched

Which statement about the toxin from B.thuringiensis is false?

A researcher inserts a DNA segment at the BamHI recognition site within a plasmid;this site is located within the tetracycline resistance gene.This plasmid also has a gene for ampicillin resistance.Following DNA transformation,the researcher must differentiate the bacteria that have taken up the recombinant DNA from those that have taken up either the DNA segment only or have taken up intact plasmids.In order to do so,the researcher should select the bacteria that

Recombinant DNA technology requires not only getting the DNA into a cell,but also getting it to replicate appropriately.In this process,the specificity of which enzyme can present a challenge?