Exam 21: Molecular Technologies

A scientist does not have an antibody to detect the protein of interest, but wants to know if the gene is expressed at low levels. What technique could the researcher use to detect, but not quantify the expression of the gene of interest?

What is the purpose of DNA ligase in a cloning experiment?

Real-time PCR can be used for which of the following?

What is one technique that is used to identify a specific RNA sequence of a sample from a library?

The isolation and copying of a gene, usually in large quantities is called gene cloning.

Cloned genes may be mutated outside of a cell by a process called site-directed mutagenesis.

Who developed the process of the polymerase chain reaction?

Restriction endonucleases recognize specific sequences in the DNA.

The DNA Pol III enzyme can use mRNA to make a cDNA strand.

Dideoxy nucleotides are used in which technique?

A researcher is interested in whether or not a transcription factor binds to a specific site on a strand of DNA. However, the samples accidentally contain SDS. What will the gel mobility shift assay results show?

The polymerase used in PCR reactions is derived from the thermophile organism Thermus aquaticus.

cDNA is made from mRNA therefore, it lacks introns and contains only the coding sequence for the functional protein.

What is the origin of restriction endonucleases?

Which of the following is an example of a palindromic DNA sequence?

What technique can be used to determine DNA-protein interactions?