Question 1

Which of the following is an example of a pluripotent cell?

Accepted Answer

A)  embryonic stem cells 
B)  red blood cells 
C)  fetal heart cells 
D)  umbilical cord blood 
E)  nerve cells 
A)  embryonic stem cells 
B)  red blood cells 
C)  fetal heart cells 
D)  umbilical cord blood 
E)  nerve cells A

Question 2

The fact that in mammalian systems multiple genes may compensate for the loss of a gene is called gene redundancy.

Accepted Answer

A) True 
 B)False  True

Question 3

A possible explanation for a lack of observable phenotypic change in a knockout mouse may involve gene redundancy

Accepted Answer

A) True 
 B)False  True

Question 4

Why was the addition of methionine necessary during the production of somatostatin by recombinant bacteria?

Accepted Answer

A)  So that it was compatible with the restriction enzyme 
B)  As a selectable marker 
C)  To allow the somatostatin to link to a bacterial protein 
D)  To increase the length of the peptide to make it more commercial 
A)  So that it was compatible with the restriction enzyme 
B)  As a selectable marker 
C)  To allow the somatostatin to link to a bacterial protein 
D)  To increase the length of the peptide to make it more commercial

Question 5

Which of the following terms represents a cell that can form any other cell of the organism?

Accepted Answer

A)  Pluripotent 
B)  Totipotent 
C)  Unipotent 
A)  Pluripotent 
B)  Totipotent 
C)  Unipotent

Question 6

Unipotent cells may differentiate into all other cell types of the body.

Accepted Answer

A) True 
 B)False

Question 7

Although Dolly was only three years old, her chromosomes had the length of a 9-10 year old sheep. Which of the following best describes why this occurred?

Accepted Answer

A)  The cell lines that created Dolly were aged prematurely in the lab 
B)  Nonhomologous recombination 
C)  A mutation enhanced the rate of aging 
D)  Dolly was not a clone 
E)  The telomeres of the somatic cells that Dolly originated from were shortening 
A)  The cell lines that created Dolly were aged prematurely in the lab 
B)  Nonhomologous recombination 
C)  A mutation enhanced the rate of aging 
D)  Dolly was not a clone 
E)  The telomeres of the somatic cells that Dolly originated from were shortening

Question 8

The use of microorganisms or their products to alleviate plant disease is called biological control.

Accepted Answer

A) True 
 B)False

Question 9

What is the purpose of the cyanogen bromide (CNBr) in the manufacture of insulin and somatostatin by recombinant bacteria?

Accepted Answer

A)  Allows for the uptake of the plasmid 
B)  Enhances the activity of the restriction enzymes 
C)  Creates sticky ends for integration into the plasmid 
D)  Cleaves the protein from the beta-galactosidase protein 
A)  Allows for the uptake of the plasmid 
B)  Enhances the activity of the restriction enzymes 
C)  Creates sticky ends for integration into the plasmid 
D)  Cleaves the protein from the beta-galactosidase protein

Question 10

Lipid vesicles that are used to introduce DNA into a cell for gene therapy are called liposomes.

Accepted Answer

A) True 
 B)False

Question 11

In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.

Accepted Answer

A) True 
 B)False

Question 12

Reproductive cloning is used to produce large amounts of mammalian proteins from transgenic agricultural animals such as cattle.

Accepted Answer

A) True 
 B)False

Question 13

The first human diseases that was successfully treated using gene therapy was ________.

Accepted Answer

A)  Diabetes 
B)  Cystic fibrosis 
C)  Severe combined immunodeficiency diseases 
D)  Muscular dystrophy 
A)  Diabetes 
B)  Cystic fibrosis 
C)  Severe combined immunodeficiency diseases 
D)  Muscular dystrophy

Question 14

A bone marrow transplant involves the transfer of multipotent stem cells

Accepted Answer

A) True 
 B)False

Question 15

Cells that have undergone homologous recombination for gene replacement would have which of the following gene combinations?

Accepted Answer

A)  Only the cloned gene 
B)  Only the original gene 
C)  Both the cloned and original gene 
D)  Neither the cloned or original gene 
A)  Only the cloned gene 
B)  Only the original gene 
C)  Both the cloned and original gene 
D)  Neither the cloned or original gene

Question 16

The reduction of pollutants in the environment by microorganisms is called biodegradation.

Accepted Answer

A) True 
 B)False

Question 17

The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic organism is called electroporation.

Accepted Answer

A) True 
 B)False

Question 18

Which of the following cells is not pluripotent?

Accepted Answer

A)  ES cells 
B)  EG cells 
C)  None of the answers are pluripotent 
D)  Both ES and EG are pluripotent 
A)  ES cells 
B)  EG cells 
C)  None of the answers are pluripotent 
D)  Both ES and EG are pluripotent

Question 19

The origins of which of the following cell types creates the least amount of ethical debate?

Accepted Answer

A)  EG cells 
B)  EC cells 
C)  Hematopoietic stem cells 
D)  ES cells 
A)  EG cells 
B)  EC cells 
C)  Hematopoietic stem cells 
D)  ES cells

Question 20

Bioremediation has been used to treat which of the following?

Accepted Answer

A)  Oil spills 
B)  Sewage 
C)  Pesticides 
D)  Heavy metals 
E)  All of the answers have been successfully remediated 
A)  Oil spills 
B)  Sewage 
C)  Pesticides 
D)  Heavy metals 
E)  All of the answers have been successfully remediated

Exam 22: Biotechnology

Which of the following is an example of a pluripotent cell?

The fact that in mammalian systems multiple genes may compensate for the loss of a gene is called gene redundancy.

A possible explanation for a lack of observable phenotypic change in a knockout mouse may involve gene redundancy

Why was the addition of methionine necessary during the production of somatostatin by recombinant bacteria?

Which of the following terms represents a cell that can form any other cell of the organism?

Unipotent cells may differentiate into all other cell types of the body.

Although Dolly was only three years old, her chromosomes had the length of a 9-10 year old sheep. Which of the following best describes why this occurred?

The use of microorganisms or their products to alleviate plant disease is called biological control.

What is the purpose of the cyanogen bromide (CNBr) in the manufacture of insulin and somatostatin by recombinant bacteria?

Lipid vesicles that are used to introduce DNA into a cell for gene therapy are called liposomes.

In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.

Reproductive cloning is used to produce large amounts of mammalian proteins from transgenic agricultural animals such as cattle.

The first human diseases that was successfully treated using gene therapy was ________.

A bone marrow transplant involves the transfer of multipotent stem cells

Cells that have undergone homologous recombination for gene replacement would have which of the following gene combinations?

The reduction of pollutants in the environment by microorganisms is called biodegradation.

The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic organism is called electroporation.

Which of the following cells is not pluripotent?

The origins of which of the following cell types creates the least amount of ethical debate?

Bioremediation has been used to treat which of the following?

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional and Translation Regulation

Gene Regulation in Eukaryotes Ii: Epigenetics

Non-Coding Rnas

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination, Immunogenetics, and Transposition

Molecular Technologies

Genomics I: Analysis of Dna

Genomics Ii: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Complex and Quantitative Traits

Evolutionary Genetics

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