Question 1

Choose the correct answer from the list below. Not all of the answers will be used.
-In order to sequence a whole protein, ____________ are used.

Accepted Answer

A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography 
A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography

Question 2

Which of the following techniques can be used to determine the size of a target protein?

Accepted Answer

A)  Edman degradation 
B)  Affinity chromatography 
C)  SDS polyacrylamide gel electrophoresis 
D)  ELISA 
E)  Isoelectric-focusing gel 
A)  Edman degradation 
B)  Affinity chromatography 
C)  SDS polyacrylamide gel electrophoresis 
D)  ELISA 
E)  Isoelectric-focusing gel

Question 3

Which of the following is true regarding gel-filtration chromatography and PAGE?

Accepted Answer

A)  In both, small proteins move most rapidly. 
B)  In both, large proteins move most rapidly. 
C)  In PAGE, large proteins move most rapidly but in gel filtration, small proteins move most rapidly. 
D)  In gel filtration, large proteins move most rapidly, but in PAGE, small proteins move most rapidly. 
E)  None of the above. 
A)  In both, small proteins move most rapidly. 
B)  In both, large proteins move most rapidly. 
C)  In PAGE, large proteins move most rapidly but in gel filtration, small proteins move most rapidly. 
D)  In gel filtration, large proteins move most rapidly, but in PAGE, small proteins move most rapidly. 
E)  None of the above.

Question 4

Choose the correct answer from the list below. Not all of the answers will be used.
-____________: The separation of proteins based first on charge then size.

Accepted Answer

A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography 
A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography

Exam 5: Techniques in Protein Biochemistry

Choose the correct answer from the list below. Not all of the answers will be used. -In order to sequence a whole protein, ____________ are used.

Which of the following techniques can be used to determine the size of a target protein?

Which of the following is true regarding gel-filtration chromatography and PAGE?

Choose the correct answer from the list below. Not all of the answers will be used. -____________: The separation of proteins based first on charge then size.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

Recombinant DNA Techniques

Filters