Question 1

When enzymes are purified, the assay is often based on:

Accepted Answer

A)  light absorbance. 
B)  catalytic activity. 
C)  pH. 
D)  temperature changes. 
E)  mRNA levels. 
A)  light absorbance. 
B)  catalytic activity. 
C)  pH. 
D)  temperature changes. 
E)  mRNA levels.

Question 2

________________ gels are often used as the media for electrophoretic techniques such as SDS-PAGE and isoelectric focusing.

Accepted Answer

The answer of ________________ gels are often used as the...

Question 3

Choose the correct answer from the list below. Not all of the answers will be used.
-____________: A protein purification technique characterized by high resolution and rapid separation.

Accepted Answer

A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography 
A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography

Question 4

In amino acid composition analysis, amino acids are visualized after separation using the chemical reagent _______________.

Accepted Answer

The answer of In amino acid composition analysis, amino acids...

Question 5

How can the amino acid sequences be used to design a DNA probe?

Accepted Answer

Using the amino acid sequence,

Question 6

Proteins can be separated from small molecules and ions through a semipermeable membrane by __________________.

Accepted Answer

The answer of Proteins can be separated from small molecules...

Question 7

________________ allows the detection of small amounts of target proteins as well as the ability to determine the size of target proteins.

Accepted Answer

Western bl

Question 8

Choose the correct answer from the list below. Not all of the answers will be used.
-The subset of gene products actually expressed by the cell is ____________.

Accepted Answer

A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography 
A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography

Question 9

Choose the correct answer from the list below. Not all of the answers will be used.
-____________: A type of purification that is based on the attraction of the protein for a particular chemical group.

Accepted Answer

A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography 
A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography

Question 10

The ________________ of a protein is the pH at which its net charge is zero.

Accepted Answer

The answer of The ________________ of a protein is the...

Question 11

Molecular exclusion gel or gel-filtration chromatography separates molecules on the basis of __________________.

Accepted Answer

The answer of Molecular exclusion gel or gel-filtration chromatography separates...

Question 12

Explain the trend of specific activity as a protein is purified.

Accepted Answer

As specific activity is expressed as uni

Question 13

Cyanogen bromide cleaves the peptide bond at:

Accepted Answer

A)  the carboxyl side of Arginine and Lysine residues. 
B)  the carboxyl side of Methionine residues. 
C)  the amino terminus. 
D)  None of the above. 
E)  All of the above. 
A)  the carboxyl side of Arginine and Lysine residues. 
B)  the carboxyl side of Methionine residues. 
C)  the amino terminus. 
D)  None of the above. 
E)  All of the above.

Question 14

How is lactic acid dehydrogenase assayed?

Accepted Answer

It is assayed by the increase

Question 15

Describe the Edman degradation method for protein-sequence analysis.

Accepted Answer

A pure protein is reacted with phenyliso

Question 16

Affinity chromatography:

Accepted Answer

A)  allows high resolution and rapid separation. 
B)  separates proteins based on size. 
C)  separates proteins based on charge. 
D)  separates proteins based on attraction to another molecule. 
E)  separates proteins based on charge and size. 
A)  allows high resolution and rapid separation. 
B)  separates proteins based on size. 
C)  separates proteins based on charge. 
D)  separates proteins based on attraction to another molecule. 
E)  separates proteins based on charge and size.

Question 17

Trypsin cleaves the peptide bond at:

Accepted Answer

A)  the carboxyl side of Arginine and Lysine residues. 
B)  the carboxyl side of Methionine residues. 
C)  the amino terminus. 
D)  None of the above. 
E)  All of the above. 
A)  the carboxyl side of Arginine and Lysine residues. 
B)  the carboxyl side of Methionine residues. 
C)  the amino terminus. 
D)  None of the above. 
E)  All of the above.

Question 18

What is the advantage of adding SDS to gel electrophoresis?

Accepted Answer

A)  SDS colors the proteins for visualization. 
B)  SDS reduces disulfide bonds. 
C)  SDS allows proteins to be separated on the basis of approximate mass. 
D)  None of the above. 
E)  All of the above. 
A)  SDS colors the proteins for visualization. 
B)  SDS reduces disulfide bonds. 
C)  SDS allows proteins to be separated on the basis of approximate mass. 
D)  None of the above. 
E)  All of the above.

Question 19

Choose the correct answer from the list below. Not all of the answers will be used.
-Proteins with different sedimentation coefficients can be separated by ____________.

Accepted Answer

A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography 
A) HPLC 
B) specific activity 
C) MALDI-TOF mass spectrum 
D) gradient centrifugation 
E) proteome
F)SDS
G)two-dimensional electrophoresis
H)Svedberg
I)immunoglobulin
J)differential centrifugation
K)overlap peptides
L)affinity chromatography

Question 20

Proteins that are not catalysts are often assayed using:

Accepted Answer

A)  antibody-binding assays. 
B)  enzymatic activity. 
C)  amino acid analysis. 
D)  None of the above. 
E)  All of the above. 
A)  antibody-binding assays. 
B)  enzymatic activity. 
C)  amino acid analysis. 
D)  None of the above. 
E)  All of the above.

Exam 5: Techniques in Protein Biochemistry

When enzymes are purified, the assay is often based on:

________________ gels are often used as the media for electrophoretic techniques such as SDS-PAGE and isoelectric focusing.

Choose the correct answer from the list below. Not all of the answers will be used. -____________: A protein purification technique characterized by high resolution and rapid separation.

In amino acid composition analysis, amino acids are visualized after separation using the chemical reagent _______________.

How can the amino acid sequences be used to design a DNA probe?

Proteins can be separated from small molecules and ions through a semipermeable membrane by __________________.

________________ allows the detection of small amounts of target proteins as well as the ability to determine the size of target proteins.

Choose the correct answer from the list below. Not all of the answers will be used. -The subset of gene products actually expressed by the cell is ____________.

Choose the correct answer from the list below. Not all of the answers will be used. -____________: A type of purification that is based on the attraction of the protein for a particular chemical group.

The ________________ of a protein is the pH at which its net charge is zero.

Molecular exclusion gel or gel-filtration chromatography separates molecules on the basis of __________________.

Explain the trend of specific activity as a protein is purified.

Cyanogen bromide cleaves the peptide bond at:

How is lactic acid dehydrogenase assayed?

Describe the Edman degradation method for protein-sequence analysis.

Affinity chromatography:

Trypsin cleaves the peptide bond at:

What is the advantage of adding SDS to gel electrophoresis?

Choose the correct answer from the list below. Not all of the answers will be used. -Proteins with different sedimentation coefficients can be separated by ____________.

Proteins that are not catalysts are often assayed using:

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

Recombinant DNA Techniques

Filters