Exam 13: Dna Replication and Repair

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Bacteria are grown in a medium containing 15NH4Cl for a number of generations so that all of the DNA is made of fully "heavy" DNA.The bacteria are moved to a new medium and grown in 14NH4Cl so that all new DNA will be "light".If replication were dispersive,what would the DNA look like after two generation times.

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What is the average rate of replication in bacteria like E.coli?

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What enzyme is responsible for recognizing a chemical alteration in the DNA and removing the base by cleavage of the bond holding the base to the deoxyribose sugar moiety?

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The specific site on the bacterial chromosome at which replication begins is called the ________.

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Replication moves outward from the origin in ________ direction(s)and is said to be ________.

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You are the enzyme responsible for carrying out mismatch repair.You notice a mismatched pair in the DNA strand and you know it is your job to remove one of the bases in the mismatched pair.How would you decide which nucleotide to remove?

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After cuts are made on the two sides of the lesion in nucleotide excision repair,what holds the damaged part of the strand in position for a while before its final removal?

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Which DNA repair mechanism removes altered nucleotides generated by reactive chemicals present in the diet or produced by metabolism?

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The DNA strand growing toward the replication fork grows ______ in a 5'->3' direction as the replication fork advances and is called the ________.

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Who was the first to purify an enzyme from bacterial extracts that could incorporate DNA precursors into a polymer?

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What is responsible for joining the last deoxyribonucleotide added during RNA primer digestion and the 5' end of the previously synthesized and adjacent DNA fragment?

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What technique was used in the discovery of the existence in eukaryotic cells of replicons,a discovery in which single DNA molecules were shown to be replicated simultaneously at several sites along their length?

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Which eukaryotic DNA polymerase is thought to be the primary DNA-synthesizing enzyme during replication of the leading strand?

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Which eukaryotic DNA polymerase appears to be the primary DNA-synthesizing enzyme during replication of the leading strand?

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Cutting of the damaged strand on both sides of lesion by endonucleases

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All DNA polymerases lay down nucleotides in a ______ direction and move along the template in a _______ direction.

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Cyclin-dependent kinase activity stays high through mitosis after rising prior to DNA synthesis.Which of the following results from its elevated activity?

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_________ are DNA unwinding enzymes that unwind the DNA in a reaction using the energy from ________ to move along one of the DNA strands,breaking the _______ holding the two strands together.

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The small portions in which eukaryotic cells replicate their genomes are called _______.

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In bacteria,a _______ is formed when a ________ associates transiently with a _______.

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