Question 1

How do you prepare tissue for electron microscopy without using fixatives?

Accepted Answer

A) Freeze it gradually over a few hours. 
B) Freeze it rapidly. 
C) Subject it to a vacuum rapidly. 
D) Heat it to dry it rapidly. 
E) Coat it quickly in heavy metal. 
A) Freeze it gradually over a few hours. 
B) Freeze it rapidly. 
C) Subject it to a vacuum rapidly. 
D) Heat it to dry it rapidly. 
E) Coat it quickly in heavy metal.

Question 2

Pulsed-field electrophoresis is typically used to separate what molecules?

Accepted Answer

A) small DNA molecules 
B) very small DNA molecules 
C) proteins larger than 25,000 daltons 
D) DNA molecules greater than about 25 kb 
E) DNA molecules smaller than about 25 kb 
A) small DNA molecules 
B) very small DNA molecules 
C) proteins larger than 25,000 daltons 
D) DNA molecules greater than about 25 kb 
E) DNA molecules smaller than about 25 kb

Question 3

What does cloning of cDNAs allow?

Accepted Answer

A) the determination of all genes present in a cell 
B) the identification of sequences active at given time in a particular cell type 
C) the identification of genes active throughout an organism's body 
D) the sequence of all genes present in a given cell 
E) the sequences of all cellular introns 
A) the determination of all genes present in a cell 
B) the identification of sequences active at given time in a particular cell type 
C) the identification of genes active throughout an organism's body 
D) the sequence of all genes present in a given cell 
E) the sequences of all cellular introns

Question 4

What part of a microscope is responsible for altering the relative distance between the specimen and the objective,thus focusing the final image precisely on the plane of the retina?

Accepted Answer

A) condenser lens 
B) objective lens 
C) ocular lens 
D) the iris 
E) focusing knob 
A) condenser lens 
B) objective lens 
C) ocular lens 
D) the iris 
E) focusing knob

Question 5

Which of the two earliest sequencing methodologies used a chemical approach?

Accepted Answer

A) the Sanger-Coulson method 
B) the Maxam-Gilbert method 
C) the cycle sequencing method 
D) the Sanger-Gilbert method 
E) the Maxam-Coulson method 
A) the Sanger-Coulson method 
B) the Maxam-Gilbert method 
C) the cycle sequencing method 
D) the Sanger-Gilbert method 
E) the Maxam-Coulson method

Question 6

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

Accepted Answer

A) blastoderm 
B) fetus 
C) blastocyst 
D) trophectoderm 
E) inner cell mass 
A) blastoderm 
B) fetus 
C) blastocyst 
D) trophectoderm 
E) inner cell mass

Question 7

What presently supplies reduced nitrogen compounds to crop plants that are unable to fix nitrogen from the atmosphere on their own?

Accepted Answer

A) phosphates 
B) water 
C) fertilizers 
D) soil 
E) sunlight 
A) phosphates 
B) water 
C) fertilizers 
D) soil 
E) sunlight

Question 8

In the cycle sequencing method,how is the sequence of the DNA determined?

Accepted Answer

A) from autoradiograms 
B) from the order of fluorescent colors in the capillary gel 
C) from the overall color of the gel 
D) from the amount of heavy nitrogen in each band on the gel 
E) from the absorbance read in the spectrophotometer 
A) from autoradiograms 
B) from the order of fluorescent colors in the capillary gel 
C) from the overall color of the gel 
D) from the amount of heavy nitrogen in each band on the gel 
E) from the absorbance read in the spectrophotometer

Question 9

In freeze-fracture methodology,the metal-carbon coating of the freeze-fracture specimen is viewed in the electron microscope after the tissue template has been thawed,removed and discarded.What is the name of the metal-carbon construct that is viewed in the scope?

Accepted Answer

A) a model 
B) a repeat 
C) a replica 
D) an object 
E) a metallica 
A) a model 
B) a repeat 
C) a replica 
D) an object 
E) a metallica

Question 10

How do DNA molecules place themselves in a CSCl equilibrium gradient?

Accepted Answer

A) They continuously move toward the bottom of the gradient. 
B) They stop momentarily at the point in the gradient equaling their buoyant density. 
C) They continuously move toward the top of the gradient. 
D) They stop permanently at the point in the gradient equaling their buoyant density. 
E) All DNA molecules localize at the same point in the gradient. 
A) They continuously move toward the bottom of the gradient. 
B) They stop momentarily at the point in the gradient equaling their buoyant density. 
C) They continuously move toward the top of the gradient. 
D) They stop permanently at the point in the gradient equaling their buoyant density. 
E) All DNA molecules localize at the same point in the gradient.

Question 11

What is the name of the procedure in which single-stranded DNA molecules are separated by electrophoresis,immobilized on a nitrocellulose filter and then hybridized with labeled,single-stranded DNA probes?

Accepted Answer

A) Southern blot 
B) Northern blot 
C) Western blot 
D) Southwestern blot 
E) Eastern blot 
A) Southern blot 
B) Northern blot 
C) Western blot 
D) Southwestern blot 
E) Eastern blot

Question 12

What is defined as a technique for producing large quantities of a specific DNA sequence? One can make millions of copies of recombinant DNA in a short period of time from one or a few initial copies.

Accepted Answer

A) DNA reproduction 
B) DNA cloning 
C) DNA replication 
D) DNA transcription 
E) DNA copying 
A) DNA reproduction 
B) DNA cloning 
C) DNA replication 
D) DNA transcription 
E) DNA copying

Question 13

In which method of transfection are cells treated with DNA that is bound to positively charged lipids,called cationic liposomes,that are capable of fusing with the lipid bilayer of the cell membrane and delivering the DNA to the cytoplasm?

Accepted Answer

A) transduction 
B) transfection 
C) electroporation 
D) lipofection 
E) transgenics 
A) transduction 
B) transfection 
C) electroporation 
D) lipofection 
E) transgenics

Question 14

Which technique depends on the use of antibodies made specifically against a particular protein (antigen)? The antibodies are then conjugated to a substance that makes them visible under the light or electron microscope.

Accepted Answer

A) immunolocalization 
B) immunocompetence 
C) immunization 
D) immunofenestration 
E) negative staining 
A) immunolocalization 
B) immunocompetence 
C) immunization 
D) immunofenestration 
E) negative staining

Question 15

Which technique could be used for molecular weight (mass)determinations in proteins?

Accepted Answer

A) X- ray diffraction 
B) chromatofocusing 
C) diethylaminoethyl (DEA
E) cellulose chromatography 
D) affinity chromatography 
E) SDS-PAGE 
A) X- ray diffraction 
B) chromatofocusing 
C) diethylaminoethyl (DEA
E) cellulose chromatography 
D) affinity chromatography 
E) SDS-PAGE

Question 16

What is usually the first step in the purification of DNA?

Accepted Answer

A) homogenization of cells and isolation of nuclei 
B) fixation of the cells 
C) isolation of mitochondria and chloroplasts 
D) crystallization of DNA 
E) removal of deoxyribonucleases 
A) homogenization of cells and isolation of nuclei 
B) fixation of the cells 
C) isolation of mitochondria and chloroplasts 
D) crystallization of DNA 
E) removal of deoxyribonucleases

How do you prepare tissue for electron microscopy without using fixatives?

Pulsed-field electrophoresis is typically used to separate what molecules?

What does cloning of cDNAs allow?

What part of a microscope is responsible for altering the relative distance between the specimen and the objective,thus focusing the final image precisely on the plane of the retina?

Which of the two earliest sequencing methodologies used a chemical approach?

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

What presently supplies reduced nitrogen compounds to crop plants that are unable to fix nitrogen from the atmosphere on their own?

In the cycle sequencing method,how is the sequence of the DNA determined?

In freeze-fracture methodology,the metal-carbon coating of the freeze-fracture specimen is viewed in the electron microscope after the tissue template has been thawed,removed and discarded.What is the name of the metal-carbon construct that is viewed in the scope?

How do DNA molecules place themselves in a C_SCl equilibrium gradient?

What is the name of the procedure in which single-stranded DNA molecules are separated by electrophoresis,immobilized on a nitrocellulose filter and then hybridized with labeled,single-stranded DNA probes?

What is defined as a technique for producing large quantities of a specific DNA sequence? One can make millions of copies of recombinant DNA in a short period of time from one or a few initial copies.

In which method of transfection are cells treated with DNA that is bound to positively charged lipids,called cationic liposomes,that are capable of fusing with the lipid bilayer of the cell membrane and delivering the DNA to the cytoplasm?

Which technique depends on the use of antibodies made specifically against a particular protein (antigen)? The antibodies are then conjugated to a substance that makes them visible under the light or electron microscope.

Which technique could be used for molecular weight (mass)determinations in proteins?

What is usually the first step in the purification of DNA?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes, and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Exam 18: Techniques in Cell and Molecular Biology

How do you prepare tissue for electron microscopy without using fixatives?

Pulsed-field electrophoresis is typically used to separate what molecules?

What does cloning of cDNAs allow?

What part of a microscope is responsible for altering the relative distance between the specimen and the objective,thus focusing the final image precisely on the plane of the retina?

Which of the two earliest sequencing methodologies used a chemical approach?

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

What presently supplies reduced nitrogen compounds to crop plants that are unable to fix nitrogen from the atmosphere on their own?

In the cycle sequencing method,how is the sequence of the DNA determined?

In freeze-fracture methodology,the metal-carbon coating of the freeze-fracture specimen is viewed in the electron microscope after the tissue template has been thawed,removed and discarded.What is the name of the metal-carbon construct that is viewed in the scope?

How do DNA molecules place themselves in a CSCl equilibrium gradient?

What is the name of the procedure in which single-stranded DNA molecules are separated by electrophoresis,immobilized on a nitrocellulose filter and then hybridized with labeled,single-stranded DNA probes?

What is defined as a technique for producing large quantities of a specific DNA sequence? One can make millions of copies of recombinant DNA in a short period of time from one or a few initial copies.

In which method of transfection are cells treated with DNA that is bound to positively charged lipids,called cationic liposomes,that are capable of fusing with the lipid bilayer of the cell membrane and delivering the DNA to the cytoplasm?

Which technique depends on the use of antibodies made specifically against a particular protein (antigen)? The antibodies are then conjugated to a substance that makes them visible under the light or electron microscope.

Which technique could be used for molecular weight (mass)determinations in proteins?

What is usually the first step in the purification of DNA?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics, Enzymes, and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

How do DNA molecules place themselves in a C_SCl equilibrium gradient?