Question 1

Nanopore technology is speedy and handles long DNA molecules.

Accepted Answer

A) True 
 B)False  True

Question 2

DNA chip technology relies on the hybridization ofـــــــــــــــــ .

Accepted Answer

A) DNA to DNA 
B) DNA to RNA 
C) RNA to DNA 
D) RNA to RNA 
A) DNA to DNA 
B) DNA to RNA 
C) RNA to DNA 
D) RNA to RNA A

Question 3

Dideoxy nucleotides are missing a 3' hydroxyl.

Accepted Answer

A) True 
 B)False  True

Question 4

Which sequencing method would work best for determining the sequence of a single DNA molecule?

Accepted Answer

A) pyrosequencing 
B) third generation sequencing 
C) second generation sequencing 
D) chain termination sequencing 
A) pyrosequencing 
B) third generation sequencing 
C) second generation sequencing 
D) chain termination sequencing

Question 5

Which of the following is a method for detecting the addition of nucleotides in a sequencing reaction?

Accepted Answer

A) luminescence 
B) fluorescence 
C) changes in pH 
D) all of the above 
A) luminescence 
B) fluorescence 
C) changes in pH 
D) all of the above

Question 6

DNA moves across nanopore detectors because ـــــــــــــــــــــ.

Accepted Answer

A) an electrical charge is applied 
B) there is a high concentration of salt on one side of the channel 
C) there is a difference in pH across the membrane 
D) it is physically pulled across by enzymes 
A) an electrical charge is applied 
B) there is a high concentration of salt on one side of the channel 
C) there is a difference in pH across the membrane 
D) it is physically pulled across by enzymes

Question 7

Nanopore detectors contain pores that only allow ــــــــــــــ to pass through.

Accepted Answer

A) hydrogen ions 
B) single-stranded DNA 
C) double-stranded DNA 
D) dideoxy nucleotides 
A) hydrogen ions 
B) single-stranded DNA 
C) double-stranded DNA 
D) dideoxy nucleotides

Question 8

All of the following are second generation sequencing methods exceptـــــــــــــ    .

Accepted Answer

A) 454 sequencing 
B) Illumina/Solexa sequencing 
C) SOLiD/Applied Biosystems method 
D) Pyrosequencing 
A) 454 sequencing 
B) Illumina/Solexa sequencing 
C) SOLiD/Applied Biosystems method 
D) Pyrosequencing

Question 9

Which method is most useful for sequencing short DNA regions from multiple individuals?

Accepted Answer

A) dideoxy sequencing 
B) pyrosequencing 
C) primer walking 
D) SMRT sequencing 
A) dideoxy sequencing 
B) pyrosequencing 
C) primer walking 
D) SMRT sequencing

Question 10

When DNA polymerase inserts a dideoxy nucleotide,ـــــــــــــــــ.

Accepted Answer

A) chain elongation continues until the end of the template 
B) energy is produced during the reaction 
C) chain elongation is terminated 
D) a primer is formed for the next round of sequencing 
A) chain elongation continues until the end of the template 
B) energy is produced during the reaction 
C) chain elongation is terminated 
D) a primer is formed for the next round of sequencing

Nanopore technology is speedy and handles long DNA molecules.

DNA chip technology relies on the hybridization ofـــــــــــــــــ .

Dideoxy nucleotides are missing a 3' hydroxyl.

Which sequencing method would work best for determining the sequence of a single DNA molecule?

Which of the following is a method for detecting the addition of nucleotides in a sequencing reaction?

DNA moves across nanopore detectors because ـــــــــــــــــــــ.

Nanopore detectors contain pores that only allow ــــــــــــــ to pass through.

All of the following are second generation sequencing methods exceptـــــــــــــ .

Which method is most useful for sequencing short DNA regions from multiple individuals?

When DNA polymerase inserts a dideoxy nucleotide,ـــــــــــــــــ.

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Manipulation of Nucleic Acids

Polymerase Chain Reaction

Recombinant DNA Technology

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Proteomics

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Analysis of Gene Expression

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters

Exam 8: DNA Sequencing

Nanopore technology is speedy and handles long DNA molecules.

DNA chip technology relies on the hybridization ofـــــــــــــــــ .

Dideoxy nucleotides are missing a 3' hydroxyl.

Which sequencing method would work best for determining the sequence of a single DNA molecule?

Which of the following is a method for detecting the addition of nucleotides in a sequencing reaction?

DNA moves across nanopore detectors because ـــــــــــــــــــــ.

Nanopore detectors contain pores that only allow ــــــــــــــ to pass through.

All of the following are second generation sequencing methods exceptـــــــــــــ .

Which method is most useful for sequencing short DNA regions from multiple individuals?

When DNA polymerase inserts a dideoxy nucleotide,ـــــــــــــــــ.

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Manipulation of Nucleic Acids

Polymerase Chain Reaction

Recombinant DNA Technology

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Proteomics

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Analysis of Gene Expression

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters