Question 1

When a virus displays multiple peptide sequences,this is termed ـــــــــــــــــــــ.

Accepted Answer

A) phage array 
B) yeast two hybrid system 
C) phage display 
D) protein microarray 
A) phage array 
B) yeast two hybrid system 
C) phage display 
D) protein microarray C

Question 2

Inteinsـــــــــــــــــــــــــ .

Accepted Answer

A) are protein self-splicing sequences 
B) are portions of mRNA that are spliced out of the primary transcript 
C) are never translated 
D) are protein sequences that are part of functional proteins 
A) are protein self-splicing sequences 
B) are portions of mRNA that are spliced out of the primary transcript 
C) are never translated 
D) are protein sequences that are part of functional proteins A

Question 3

A method that uses an antibody to detect a specific protein is called ـــــــــــــــــــــــــ.

Accepted Answer

A) SDS-PAGE 
B) Western blot 
C) Mass Spectrometry 
D) Yeast two hybrid system 
A) SDS-PAGE 
B) Western blot 
C) Mass Spectrometry 
D) Yeast two hybrid system B

Question 4

Why might proteins be tagged?

Accepted Answer

A) purification 
B) identification 
C) anchoring 
D) all of the above 
A) purification 
B) identification 
C) anchoring 
D) all of the above

Question 5

How are proteins denatured in SDS-PAGE?

Accepted Answer

A) detergent and high temperature 
B) high temperature only 
C) acylamide 
D) electrical charge 
A) detergent and high temperature 
B) high temperature only 
C) acylamide 
D) electrical charge

Question 6

What is the proteome?

Accepted Answer

A) The total set of proteins in the cell at any given point. 
B) The number of genes that are expressed in a specific growth condition. 
C) The total set of proteins encoded by the organism's genome. 
D) The number of expressed genes. 
A) The total set of proteins in the cell at any given point. 
B) The number of genes that are expressed in a specific growth condition. 
C) The total set of proteins encoded by the organism's genome. 
D) The number of expressed genes.

Question 7

The primary antibody in a Western blotـــــــــــــــــــــ .

Accepted Answer

A) binds to the nitrocellulose membrane 
B) is conjugated to a detection system 
C) is used to block non-specific binding of the secondary antibody 
D) binds to the specific protein 
A) binds to the nitrocellulose membrane 
B) is conjugated to a detection system 
C) is used to block non-specific binding of the secondary antibody 
D) binds to the specific protein

Question 8

Proteins microarrays ـــــــــــــــــــــــ.

Accepted Answer

A) are used to investigate protein-protein interactions 
B) are used to determine enzymatic activity 
C) help determine the binding of DNA or RNA to protein 
D) all of the above 
A) are used to investigate protein-protein interactions 
B) are used to determine enzymatic activity 
C) help determine the binding of DNA or RNA to protein 
D) all of the above

Question 9

All of the following are appropriate methods for investigating protein-protein interactions exceptــــــــــــــــــــــ .

Accepted Answer

A) mass spectrometry 
B) two hybrid analysis 
C) electrophoretic mobility shift assays 
D) co-immunoprecipitation 
A) mass spectrometry 
B) two hybrid analysis 
C) electrophoretic mobility shift assays 
D) co-immunoprecipitation

Question 10

Which method allows a researcher to find a gene based upon the protein that it encodes?

Accepted Answer

A) Co-immunoprecipitation 
B) Phage display 
C) RT-PCR 
D) Intein trapping 
A) Co-immunoprecipitation 
B) Phage display 
C) RT-PCR 
D) Intein trapping

When a virus displays multiple peptide sequences,this is termed ـــــــــــــــــــــ.

Inteinsـــــــــــــــــــــــــ .

A method that uses an antibody to detect a specific protein is called ـــــــــــــــــــــــــ.

Why might proteins be tagged?

How are proteins denatured in SDS-PAGE?

What is the proteome?

The primary antibody in a Western blotـــــــــــــــــــــ .

Proteins microarrays ـــــــــــــــــــــــ.

All of the following are appropriate methods for investigating protein-protein interactions exceptــــــــــــــــــــــ .

Which method allows a researcher to find a gene based upon the protein that it encodes?

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Manipulation of Nucleic Acids

Polymerase Chain Reaction

Recombinant DNA Technology

DNA Sequencing

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Analysis of Gene Expression

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters

Exam 15: Proteomics

When a virus displays multiple peptide sequences,this is termed ـــــــــــــــــــــ.

Inteinsـــــــــــــــــــــــــ .

A method that uses an antibody to detect a specific protein is called ـــــــــــــــــــــــــ.

Why might proteins be tagged?

How are proteins denatured in SDS-PAGE?

What is the proteome?

The primary antibody in a Western blotـــــــــــــــــــــ .

Proteins microarrays ـــــــــــــــــــــــ.

All of the following are appropriate methods for investigating protein-protein interactions exceptــــــــــــــــــــــ .

Which method allows a researcher to find a gene based upon the protein that it encodes?

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Manipulation of Nucleic Acids

Polymerase Chain Reaction

Recombinant DNA Technology

DNA Sequencing

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Analysis of Gene Expression

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters