Question 1

Which of the following uses an RNA template during the technique?

Accepted Answer

A) TA cloning 
B) RAPD 
C) Inverse PCR 
D) RT-PCR 
A) TA cloning 
B) RAPD 
C) Inverse PCR 
D) RT-PCR D

Question 2

In real time PCR,the presence of double-stranded DNA is detected byــــــــــــــــــــــــ .

Accepted Answer

A) fluorescence of SYBR Green 
B) luminescence of SYBER Green 
C) quenching of SYBR Green fluorescence 
D) rapid decrease in the amount of SYBR Green fluorescence 
A) fluorescence of SYBR Green 
B) luminescence of SYBER Green 
C) quenching of SYBR Green fluorescence 
D) rapid decrease in the amount of SYBR Green fluorescence A

Question 3

Why is Escherichia coli DNA polymerase not very useful for PCR?

Accepted Answer

A) E.coli DNA polymerase cannot proofread during replication. 
B) E.coli DNA polymerase only amplifies circular DNA. 
C) E.coli DNA polymerase is heat labile. 
D) E.coli DNA polymerase is too slow. 
A) E.coli DNA polymerase cannot proofread during replication. 
B) E.coli DNA polymerase only amplifies circular DNA. 
C) E.coli DNA polymerase is heat labile. 
D) E.coli DNA polymerase is too slow. C

Question 4

In addition to DNA polymerase,a PCR reaction mixture contains all of the following exceptــــــــــــــــــــــــ .

Accepted Answer

A) Buffer 
B) Primers 
C) Deoxynucleotide triphosphates 
D) Ligase 
A) Buffer 
B) Primers 
C) Deoxynucleotide triphosphates 
D) Ligase

Question 5

Which component of the PCR reaction mixture confers specificity in the reaction for the target sequence on the original DNA template?

Accepted Answer

A) Taq polymerase 
B) primers 
C) nucleotides 
D) template DNA 
A) Taq polymerase 
B) primers 
C) nucleotides 
D) template DNA

Question 6

Messenger RNA from eukaryotic cells can be specifically amplified usingــــــــــــــــــــــ .

Accepted Answer

A) Standard PCR 
B) RT-PCR 
C) Differential display PCR 
D) RACE 
A) Standard PCR 
B) RT-PCR 
C) Differential display PCR 
D) RACE

Question 7

During which step in PCR does synthesis occur?

Accepted Answer

A) Denaturation 
B) Annealing 
C) Elongation 
D) Termination 
A) Denaturation 
B) Annealing 
C) Elongation 
D) Termination

Question 8

Why must DNA be denatured during PCR?

Accepted Answer

A) DNA polymerases work best at higher temperatures. 
B) Denaturing DNA separates the strands of DNA and allows primers to bind to complementary sequences. 
C) The degraded DNA nucleotides are recycled during the synthesis step of PCR. 
D) DNA does not need to be denatured for PCR to work. 
A) DNA polymerases work best at higher temperatures. 
B) Denaturing DNA separates the strands of DNA and allows primers to bind to complementary sequences. 
C) The degraded DNA nucleotides are recycled during the synthesis step of PCR. 
D) DNA does not need to be denatured for PCR to work.

Question 9

What is the function of the RNA primer during DNA synthesis?

Accepted Answer

A) DNA polymerase requires a 3' hydroxyl to initiate synthesis. 
B) The primer helps the replication machinery find the origin of replication. 
C) The primer is required for cleavage of the DNA to generate a loose end. 
D) The primer signals DNA polymerase to terminate synthesis. 
A) DNA polymerase requires a 3' hydroxyl to initiate synthesis. 
B) The primer helps the replication machinery find the origin of replication. 
C) The primer is required for cleavage of the DNA to generate a loose end. 
D) The primer signals DNA polymerase to terminate synthesis.

Question 10

The enzyme that converts RNA back into a DNA copy called complementary DNA cDNA)is calledـــــــــــــــــــــــ .

Accepted Answer

A) DNA polymerase 
B) RNA polymerase 
C) cDNA polymerase 
D) Reverse transcriptase 
A) DNA polymerase 
B) RNA polymerase 
C) cDNA polymerase 
D) Reverse transcriptase

Question 11

RNA can be synthesized in PCR if RNA polymerase and RNA nucleotides are used in place of DNA polymerase and DNA nucleotides,respectively.

Accepted Answer

A) True 
 B)False

Which of the following uses an RNA template during the technique?

In real time PCR,the presence of double-stranded DNA is detected byــــــــــــــــــــــــ .

Why is Escherichia coli DNA polymerase not very useful for PCR?

In addition to DNA polymerase,a PCR reaction mixture contains all of the following exceptــــــــــــــــــــــــ .

Which component of the PCR reaction mixture confers specificity in the reaction for the target sequence on the original DNA template?

Messenger RNA from eukaryotic cells can be specifically amplified usingــــــــــــــــــــــ .

During which step in PCR does synthesis occur?

Why must DNA be denatured during PCR?

What is the function of the RNA primer during DNA synthesis?

The enzyme that converts RNA back into a DNA copy called complementary DNA cDNA)is calledـــــــــــــــــــــــ .

RNA can be synthesized in PCR if RNA polymerase and RNA nucleotides are used in place of DNA polymerase and DNA nucleotides,respectively.

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Manipulation of Nucleic Acids

Recombinant DNA Technology

DNA Sequencing

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Proteomics

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Analysis of Gene Expression

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

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Exam 6: Polymerase Chain Reaction

Which of the following uses an RNA template during the technique?

In real time PCR,the presence of double-stranded DNA is detected byــــــــــــــــــــــــ .

Why is Escherichia coli DNA polymerase not very useful for PCR?

In addition to DNA polymerase,a PCR reaction mixture contains all of the following exceptــــــــــــــــــــــــ .

Which component of the PCR reaction mixture confers specificity in the reaction for the target sequence on the original DNA template?

Messenger RNA from eukaryotic cells can be specifically amplified usingــــــــــــــــــــــ .

During which step in PCR does synthesis occur?

Why must DNA be denatured during PCR?

What is the function of the RNA primer during DNA synthesis?

The enzyme that converts RNA back into a DNA copy called complementary DNA cDNA)is calledـــــــــــــــــــــــ .

RNA can be synthesized in PCR if RNA polymerase and RNA nucleotides are used in place of DNA polymerase and DNA nucleotides,respectively.

Cells and Organisms

Basic Genetics

DNA, RNA, and Protein

Genes, genomes, and DNA

Manipulation of Nucleic Acids

Recombinant DNA Technology

DNA Sequencing

Genomics Systems Biology

Cell Division and DNA Replication

Transcription of Genes

Processing of RNA

Protein Synthesis

Protein Structure and Function

Proteomics

Regulation of Transcription in Prokaryotes

Regulation of Transcription in Eukaryotes

Regulation at the RNA Level

Analysis of Gene Expression

Plasmids

Viruses

Mobile DNA

Mutations and Repair

Recombination

Bacterial Genetics

Molecular Evolution

Filters