Question 1

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 2

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Accepted Answer

Immersion oil increases resolution but n

Question 3

All of the following are examples of basic dyes except

Accepted Answer

A) methylene blue. 
B) nigrosin. 
C) crystal violet. 
D) safranin. 
E) carbol fuchsin. 
A) methylene blue. 
B) nigrosin. 
C) crystal violet. 
D) safranin. 
E) carbol fuchsin.

Question 4

Mixed cultures are also referred to as contaminated cultures.

Accepted Answer

A) True 
 B)False

Question 5

All of the following are correct about agar except

Accepted Answer

A) it is flexible. 
B) it melts at the boiling point of water (100°
C) . 
C) it is a source of nutrition for bacteria. 
D) it solidifies below 42°C. 
E) it is solid at room temperature. 
A) it is flexible. 
B) it melts at the boiling point of water (100°
C) . 
C) it is a source of nutrition for bacteria. 
D) it solidifies below 42°C. 
E) it is solid at room temperature.

Question 6

Some microbes are not capable of growing on artificial media.

Accepted Answer

A) True 
 B)False

Question 7

Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 8

The type of microscope in which you would see brightly illuminated specimens against a black background is

Accepted Answer

A) bright-field. 
B) dark-field. 
C) phase-contrast. 
D) fluorescence. 
E) electron. 
A) bright-field. 
B) dark-field. 
C) phase-contrast. 
D) fluorescence. 
E) electron.

Question 9

All of the following pertain to the fluorescence microscope except it

Accepted Answer

A) uses electrons to produce a specimen image. 
B) is a type of compound microscope. 
C) requires the use of dyes like acridine and fluorescein. 
D) is commonly used to diagnose certain infections. 
E) requires an ultraviolet radiation source. 
A) uses electrons to produce a specimen image. 
B) is a type of compound microscope. 
C) requires the use of dyes like acridine and fluorescein. 
D) is commonly used to diagnose certain infections. 
E) requires an ultraviolet radiation source.

Question 10

A bacterial cell that is deposited on the surface of an appropriate solid growth media will divide repeatedly until it forms a visible accumulation of bacterial growth.This is known as a bacterial

Accepted Answer

A) colony. 
B) community. 
C) biofilm. 
D) smear. 
A) colony. 
B) community. 
C) biofilm. 
D) smear.

Question 11

Which microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 12

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the_____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

The answer of Magnification is achieved in a compound microscope...

Question 13

The procedure for culturing a microorganism requires the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 14

Brain-heart infusion, trypticase soy agar (TSA), and nutrient agar are all examples of which type of media?

Accepted Answer

A) synthetic 
B) reducing 
C) enriched 
D) complex 
E) selective 
A) synthetic 
B) reducing 
C) enriched 
D) complex 
E) selective

Question 15

The primary purpose of staining cells on a microscope slide is to

Accepted Answer

A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility. 
A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility.

Question 16

Observing and characterizing colonial growth for size, shape, edge, elevation, color, odor, and texture is part of ___.

Accepted Answer

A) specimen collection 
B) inoculation 
C) incubation 
D) isolation 
E) inspection 
A) specimen collection 
B) inoculation 
C) incubation 
D) isolation 
E) inspection

Question 17

Which microscope does not use light in forming the specimen image?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 18

Basic dyes are

Accepted Answer

A) attracted to the acidic substances of bacterial cells. 
B) anionic. 
C) used in negative staining. 
D) repelled by cells. 
E) dyes such as India ink and nigrosin. 
A) attracted to the acidic substances of bacterial cells. 
B) anionic. 
C) used in negative staining. 
D) repelled by cells. 
E) dyes such as India ink and nigrosin.

Question 19

_____ is the term for a starting a new culture from an existing one.

Accepted Answer

The answer of _____ is the term for a starting...

Question 20

All of the following are criteria that are used to classify different types of microbiological growth media EXCEPT

Accepted Answer

A) physical state or consistency. 
B) purpose or function. 
C) chemical composition. 
D) sensory characteristics (color, smell). 
A) physical state or consistency. 
B) purpose or function. 
C) chemical composition. 
D) sensory characteristics (color, smell).

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

A medium that is gel-like has less agar in it compared to a solid medium.

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

All of the following are examples of basic dyes except

Mixed cultures are also referred to as contaminated cultures.

All of the following are correct about agar except

Some microbes are not capable of growing on artificial media.

Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?

The type of microscope in which you would see brightly illuminated specimens against a black background is

All of the following pertain to the fluorescence microscope except it

A bacterial cell that is deposited on the surface of an appropriate solid growth media will divide repeatedly until it forms a visible accumulation of bacterial growth.This is known as a bacterial

Which microscope achieves the greatest resolution and highest magnification?

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the_____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

The procedure for culturing a microorganism requires the use of a microscope.

Brain-heart infusion, trypticase soy agar (TSA), and nutrient agar are all examples of which type of media?

The primary purpose of staining cells on a microscope slide is to

Observing and characterizing colonial growth for size, shape, edge, elevation, color, odor, and texture is part of ___.

Which microscope does not use light in forming the specimen image?

Basic dyes are

_____ is the term for a starting a new culture from an existing one.

All of the following are criteria that are used to classify different types of microbiological growth media EXCEPT

The Main Themes of Microbiology

The Chemistry of Biology

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

Procedures for Identifying Pathogens and Diagnosing Infections

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

Filters

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the_ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.