Question 1

What is meant by semiconservative replication of DNA?

Accepted Answer

A)  Only part of the molecule is reproduced at one time. 
B)  Each strand is the template for another strand. 
C)  One copy is the same, and another copy is different. 
D)  DNA uses RNA to make new copies. 
A)  Only part of the molecule is reproduced at one time. 
B)  Each strand is the template for another strand. 
C)  One copy is the same, and another copy is different. 
D)  DNA uses RNA to make new copies.

Question 2

In the process of translation,the addition of a particular amino acid to a growing protein may be specified by:

Accepted Answer

A)  one specific mRNA nucleotide. 
B)  only one specific combination of three mRNA nucleotides. 
C)  one of several specific combinations of three mRNA nucleotides. 
A)  one specific mRNA nucleotide. 
B)  only one specific combination of three mRNA nucleotides. 
C)  one of several specific combinations of three mRNA nucleotides.

Question 3

Select the reaction characterized by amplification of a specific region of a DNA strand through denaturing and annealing.

Accepted Answer

A)  Signal amplification 
B)  Polymerase chain reaction 
C)  Solution hybridization 
D)  Ligase chain reaction 
A)  Signal amplification 
B)  Polymerase chain reaction 
C)  Solution hybridization 
D)  Ligase chain reaction

Question 4

When denatured,DNA is converted from:

Accepted Answer

A)  a double-stranded double helix to a double-stranded linear structure. 
B)  a double strand to a pair of single strands. 
C)  DNA to RNA. 
D)  polymerized strands of nucleic acids to individual nucleic acids. 
A)  a double-stranded double helix to a double-stranded linear structure. 
B)  a double strand to a pair of single strands. 
C)  DNA to RNA. 
D)  polymerized strands of nucleic acids to individual nucleic acids.

Question 5

What is the difference between a mutation and a polymorphism?

Accepted Answer

A)  A mutation can exist in a single person; a polymorphism must exist in at least 2% of the population. 
B)  A mutation involves a single base pair; a polymorphism involves many base pairs. 
C)  Mutations affect cell function; polymorphisms do not. 
D)  Mutations do not change DNA structure; polymorphisms distort the helical structure. 
A)  A mutation can exist in a single person; a polymorphism must exist in at least 2% of the population. 
B)  A mutation involves a single base pair; a polymorphism involves many base pairs. 
C)  Mutations affect cell function; polymorphisms do not. 
D)  Mutations do not change DNA structure; polymorphisms distort the helical structure.

Question 6

ddNTPs terminate the lengthening of a DNA strand because they lack chemical groups that:

Accepted Answer

A)  allow hydrogen bonding to the template strand. 
B)  are recognized by DNA polymerase. 
C)  protect the strand from endonucleases. 
D)  form phosphodiester bonds in the backbone of the strand. 
A)  allow hydrogen bonding to the template strand. 
B)  are recognized by DNA polymerase. 
C)  protect the strand from endonucleases. 
D)  form phosphodiester bonds in the backbone of the strand.

Question 7

How would you interpret results of DNA restriction analysis by gel electrophoresis if there was a difference in one band?

Accepted Answer

A)  The samples are the same. 
B)  The samples are not identical. 
C)  Gel electrophoresis should be repeated. 
D)  The wrong buffer was used in electrophoresis. 
A)  The samples are the same. 
B)  The samples are not identical. 
C)  Gel electrophoresis should be repeated. 
D)  The wrong buffer was used in electrophoresis.

Question 8

Select the method that detects target DNA in intact cells.

Accepted Answer

A)  Polymerase chain reaction 
B)  Southern blot 
C)  Solution hybridization 
D)  Fluorescence in situ hybridization 
A)  Polymerase chain reaction 
B)  Southern blot 
C)  Solution hybridization 
D)  Fluorescence in situ hybridization

Question 9

What is the basis of strand displacement amplification?

Accepted Answer

A)  Two different primers bind to the same strand of DNA. 
B)  Two different primers each bind to complementary strands of DNA. 
C)  Multiple primers bind to fragmented DNA targets. 
D)  Primers are joined together. 
A)  Two different primers bind to the same strand of DNA. 
B)  Two different primers each bind to complementary strands of DNA. 
C)  Multiple primers bind to fragmented DNA targets. 
D)  Primers are joined together.

Question 10

Next-generation sequencing involves:

Accepted Answer

A)  generating many short sequences from an intact, continuous DNA sequence. 
B)  generating many short sequences from fragmented DNA. 
C)  splicing together DNA fragments. 
D)  adding multiple probes to fragmented DNA. 
A)  generating many short sequences from an intact, continuous DNA sequence. 
B)  generating many short sequences from fragmented DNA. 
C)  splicing together DNA fragments. 
D)  adding multiple probes to fragmented DNA.

Question 11

Select the probe sequence that will hybridize to the following nucleic acid sequence: C G A T A T T G T C

Accepted Answer

A)  T A G T A C A A G A 
B)  C G A T A T T G T C 
C)  G T C A A G A C C T 
D)  G C T A T A A C A G 
A)  T A G T A C A A G A 
B)  C G A T A T T G T C 
C)  G T C A A G A C C T 
D)  G C T A T A A C A G

Question 12

Which type of RNA is used to directly translate the DNA code into making functional proteins?

Accepted Answer

A)  Transfer RNA 
B)  Ribosomal RNA 
C)  Messenger RNA 
D)  Probe RNA 
A)  Transfer RNA 
B)  Ribosomal RNA 
C)  Messenger RNA 
D)  Probe RNA

Question 13

Select the strand of RNA that is complementary to this single strand of DNA: A T T A G C C G T A T

Accepted Answer

A)  T A A T C G G C A T A 
B)  U A A U C G G C U A U 
C)  T U U T C G G C A U A 
D)  T A A T U G G U A T A 
A)  T A A T C G G C A T A 
B)  U A A U C G G C U A U 
C)  T U U T C G G C A U A 
D)  T A A T U G G U A T A

What is meant by semiconservative replication of DNA?

In the process of translation,the addition of a particular amino acid to a growing protein may be specified by:

Select the reaction characterized by amplification of a specific region of a DNA strand through denaturing and annealing.

When denatured,DNA is converted from:

What is the difference between a mutation and a polymorphism?

ddNTPs terminate the lengthening of a DNA strand because they lack chemical groups that:

How would you interpret results of DNA restriction analysis by gel electrophoresis if there was a difference in one band?

Select the method that detects target DNA in intact cells.

What is the basis of strand displacement amplification?

Next-generation sequencing involves:

Select the probe sequence that will hybridize to the following nucleic acid sequence: C G A T A T T G T C

Which type of RNA is used to directly translate the DNA code into making functional proteins?

Select the strand of RNA that is complementary to this single strand of DNA: A T T A G C C G T A T

Introduction to Immunity and the Immune System

Nature of Antigens and the Major Histocompatibility Complex

Innate Immunity

Adaptive Immunity

Antibody Structure and Function

Cytokines

Complement System

Safety and Quality Management

Principles of Serological Testing

Precipitation and Agglutination Reactions

Labeled Immunoassays

Flow Cytometry and Laboratory Automation

Hypersensitivity

Autoimmunity

Transplantation Immunology

Tumor Immunology

Immunoproliferative Diseases

Immunodeficiency Diseases

Serological and Molecular Detection of Bacterial Infections

Spirochete Diseases

Serological and Molecular Diagnosis of Parasitic and Fungal Infections

Serology and Molecular Detection of Viral Infections

Laboratory Diagnosis of HIV Infection

Immunization and Vaccines

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Exam 12: Molecular Diagnostic Techniques

What is meant by semiconservative replication of DNA?

In the process of translation,the addition of a particular amino acid to a growing protein may be specified by:

Select the reaction characterized by amplification of a specific region of a DNA strand through denaturing and annealing.

When denatured,DNA is converted from:

What is the difference between a mutation and a polymorphism?

ddNTPs terminate the lengthening of a DNA strand because they lack chemical groups that:

How would you interpret results of DNA restriction analysis by gel electrophoresis if there was a difference in one band?

Select the method that detects target DNA in intact cells.

What is the basis of strand displacement amplification?

Next-generation sequencing involves:

Select the probe sequence that will hybridize to the following nucleic acid sequence: C G A T A T T G T C

Which type of RNA is used to directly translate the DNA code into making functional proteins?

Select the strand of RNA that is complementary to this single strand of DNA: A T T A G C C G T A T

Introduction to Immunity and the Immune System

Nature of Antigens and the Major Histocompatibility Complex

Innate Immunity

Adaptive Immunity

Antibody Structure and Function

Cytokines

Complement System

Safety and Quality Management

Principles of Serological Testing

Precipitation and Agglutination Reactions

Labeled Immunoassays

Flow Cytometry and Laboratory Automation

Hypersensitivity

Autoimmunity

Transplantation Immunology

Tumor Immunology

Immunoproliferative Diseases

Immunodeficiency Diseases

Serological and Molecular Detection of Bacterial Infections

Spirochete Diseases

Serological and Molecular Diagnosis of Parasitic and Fungal Infections

Serology and Molecular Detection of Viral Infections

Laboratory Diagnosis of HIV Infection

Immunization and Vaccines

Filters