Question 1

Quenching of the signal emitted by the label is of particular concern in:

Accepted Answer

A)  radioimmunoassay. 
B)  enzyme immunoassay. 
C)  fluorescence polarization. 
D)  chemiluminescent immunoassays. 
A)  radioimmunoassay. 
B)  enzyme immunoassay. 
C)  fluorescence polarization. 
D)  chemiluminescent immunoassays.

Question 2

Extremely high analyte concentrations can cause false-negative results (hook effect)in which type of assay?

Accepted Answer

A)  Homogeneous competitive 
B)  Heterogeneous competitive 
C)  Homogeneous noncompetitive 
D)  Heterogeneous noncompetitive 
A)  Homogeneous competitive 
B)  Heterogeneous competitive 
C)  Homogeneous noncompetitive 
D)  Heterogeneous noncompetitive

Question 3

An ELISA assay was performed on a patient's serum sample.What is the relationship of the amount of enzyme activity to the amount of patient analyte measured?

Accepted Answer

A)  Directly proportional 
B)  Indirectly proportional 
A)  Directly proportional 
B)  Indirectly proportional

Question 4

Which of the following best describes homogeneous assays?

Accepted Answer

A)  A washing step is not necessary. 
B)  The concentration of patient antigen is always directly proportional to the label detected. 
C)  The reagent antibody has an enzyme tag. 
D)  Labeled and unlabeled analyte are present in equal amounts. 
A)  A washing step is not necessary. 
B)  The concentration of patient antigen is always directly proportional to the label detected. 
C)  The reagent antibody has an enzyme tag. 
D)  Labeled and unlabeled analyte are present in equal amounts.

Question 5

Pregnancy testing can be performed at home using:

Accepted Answer

A)  capture assay. 
B)  immunochromatography. 
C)  fluorescence polarization. 
D)  chemiluminescence. 
A)  capture assay. 
B)  immunochromatography. 
C)  fluorescence polarization. 
D)  chemiluminescence.

Question 6

The signal measured in a fluorescence polarization immunoassay is the:

Accepted Answer

A)  wavelength of absorbed light. 
B)  angle of light scattering. 
C)  intensity of light emission. 
D)  orientation of emitted light waves. 
A)  wavelength of absorbed light. 
B)  angle of light scattering. 
C)  intensity of light emission. 
D)  orientation of emitted light waves.

Question 7

A tissue section is fixed to a microscope slide,then incubated with a fluorescently labeled antibody that binds to an antigen expressed by cells in that tissue.The preparation is then washed and inspected with a fluorescent microscope.This technique is called:

Accepted Answer

A)  direct immunofluorescence. 
B)  indirect immunofluorescence. 
C)  fluorescence polarization. 
D)  autofluorescence. 
A)  direct immunofluorescence. 
B)  indirect immunofluorescence. 
C)  fluorescence polarization. 
D)  autofluorescence.

Question 8

Which of the following best describes competitive binding assays?

Accepted Answer

A)  A limited number of binding sites are present. 
B)  The concentration of patient antigen is directly proportional to the label detected. 
C)  All patient antigen present is allowed to bind. 
D)  Labeled and unlabeled analyte are present in equal amounts. 
A)  A limited number of binding sites are present. 
B)  The concentration of patient antigen is directly proportional to the label detected. 
C)  All patient antigen present is allowed to bind. 
D)  Labeled and unlabeled analyte are present in equal amounts.

Question 9

Point-of-care testing is performed most easily using:

Accepted Answer

A)  homogeneous enzyme immunoassay. 
B)  immunofixation assay. 
C)  immunochromatographic assay. 
D)  capture ELISA. 
A)  homogeneous enzyme immunoassay. 
B)  immunofixation assay. 
C)  immunochromatographic assay. 
D)  capture ELISA.

Question 10

A good enzyme for use in an immunoassay should have a:

Accepted Answer

A)  rapid degradation rate. 
B)  high substrate conversion rate. 
C)  high cost. 
D)  toxic enzymatic product. 
A)  rapid degradation rate. 
B)  high substrate conversion rate. 
C)  high cost. 
D)  toxic enzymatic product.

Quenching of the signal emitted by the label is of particular concern in:

Extremely high analyte concentrations can cause false-negative results (hook effect)in which type of assay?

An ELISA assay was performed on a patient's serum sample.What is the relationship of the amount of enzyme activity to the amount of patient analyte measured?

Which of the following best describes homogeneous assays?

Pregnancy testing can be performed at home using:

The signal measured in a fluorescence polarization immunoassay is the:

A tissue section is fixed to a microscope slide,then incubated with a fluorescently labeled antibody that binds to an antigen expressed by cells in that tissue.The preparation is then washed and inspected with a fluorescent microscope.This technique is called:

Which of the following best describes competitive binding assays?

Point-of-care testing is performed most easily using:

A good enzyme for use in an immunoassay should have a:

Introduction to Immunity and the Immune System

Nature of Antigens and the Major Histocompatibility Complex

Innate Immunity

Adaptive Immunity

Antibody Structure and Function

Cytokines

Complement System

Safety and Quality Management

Principles of Serological Testing

Precipitation and Agglutination Reactions

Molecular Diagnostic Techniques

Flow Cytometry and Laboratory Automation

Hypersensitivity

Autoimmunity

Transplantation Immunology

Tumor Immunology

Immunoproliferative Diseases

Immunodeficiency Diseases

Serological and Molecular Detection of Bacterial Infections

Spirochete Diseases

Serological and Molecular Diagnosis of Parasitic and Fungal Infections

Serology and Molecular Detection of Viral Infections

Laboratory Diagnosis of HIV Infection

Immunization and Vaccines

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Exam 11: Labeled Immunoassays

Quenching of the signal emitted by the label is of particular concern in:

Extremely high analyte concentrations can cause false-negative results (hook effect)in which type of assay?

An ELISA assay was performed on a patient's serum sample.What is the relationship of the amount of enzyme activity to the amount of patient analyte measured?

Which of the following best describes homogeneous assays?

Pregnancy testing can be performed at home using:

The signal measured in a fluorescence polarization immunoassay is the:

A tissue section is fixed to a microscope slide,then incubated with a fluorescently labeled antibody that binds to an antigen expressed by cells in that tissue.The preparation is then washed and inspected with a fluorescent microscope.This technique is called:

Which of the following best describes competitive binding assays?

Point-of-care testing is performed most easily using:

A good enzyme for use in an immunoassay should have a:

Introduction to Immunity and the Immune System

Nature of Antigens and the Major Histocompatibility Complex

Innate Immunity

Adaptive Immunity

Antibody Structure and Function

Cytokines

Complement System

Safety and Quality Management

Principles of Serological Testing

Precipitation and Agglutination Reactions

Molecular Diagnostic Techniques

Flow Cytometry and Laboratory Automation

Hypersensitivity

Autoimmunity

Transplantation Immunology

Tumor Immunology

Immunoproliferative Diseases

Immunodeficiency Diseases

Serological and Molecular Detection of Bacterial Infections

Spirochete Diseases

Serological and Molecular Diagnosis of Parasitic and Fungal Infections

Serology and Molecular Detection of Viral Infections

Laboratory Diagnosis of HIV Infection

Immunization and Vaccines

Filters