Question 1

The presence of a specific plasmid in a bacterial cell may be determined if the plasmid _____.

Accepted Answer

A) is exceptionally large 
B) is an expression vector 
C) contains pieces of a viral genome 
D) contains a selectable marker 
A) is exceptionally large 
B) is an expression vector 
C) contains pieces of a viral genome 
D) contains a selectable marker

Question 2

A dideoxy nucleotide is missing an -OH group at the 2' end.

Accepted Answer

A) True 
 B)False

Question 3

The isolation and copying of a gene, usually in large quantities is called gene cloning.

Accepted Answer

A) True 
 B)False

Question 4

cDNA is made from mRNA therefore, it lacks introns and contains only the coding sequence for the functional protein.

Accepted Answer

A) True 
 B)False

Question 5

Dideoxy nucleotides are used in which technique?

Accepted Answer

A) RT-PCR 
B) DNA sequencing 
C) DNAse I foot printing 
D) restriction mapping 
E)  gene cloning 
A) RT-PCR 
B) DNA sequencing 
C) DNAse I foot printing 
D) restriction mapping 
E)  gene cloning

Question 6

Cloned genes may be mutated outside of a cell by a process called in vitro site-directed mutagenesis.

Accepted Answer

A) True 
 B)False

Question 7

What function does a competent cell have that makes it useful in cloning experiments?

Accepted Answer

A) Competent cells resist transfection by a viral vector. 
B) Competent cells repair DNA strands without the aid of DNA ligase. 
C) Competent cells utilize transposons as a vector. 
D) Competent cells take up DNA from the external environment. 
A) Competent cells resist transfection by a viral vector. 
B) Competent cells repair DNA strands without the aid of DNA ligase. 
C) Competent cells utilize transposons as a vector. 
D) Competent cells take up DNA from the external environment.

Question 8

R factors typically contain genes for antibiotic resistance.

Accepted Answer

A) True 
 B)False

Question 9

What is one technique that is used to identify a specific DNA sequence of a sample from a library?

Accepted Answer

A) Western blotting 
B) Northern blotting 
C) Southern blotting 
D) Eastern blotting 
A) Western blotting 
B) Northern blotting 
C) Southern blotting 
D) Eastern blotting

Question 10

___________ conditions are essential in a Southern blot experiment if the researcher wants to identify the closest DNA match to the probe.

Accepted Answer

A) High stringency 
B) Low stringency 
A) High stringency 
B) Low stringency

Question 11

Which of the following is an example of a palindromic DNA sequence?

Accepted Answer

A) 5' - ATCGAC - 3' 3' - TAGCAG - 5' 
B) 5' - ATCATC - 3' 3' - ATCATC - 5' 
C) 5' - GCCGCC - 3' 3' - CGGCGG - 5' 
D) 5' - CTGCAG - 3' 3' - GACGTC - 5' 
A) 5' - ATCGAC - 3' 3' - TAGCAG - 5' 
B) 5' - ATCATC - 3' 3' - ATCATC - 5' 
C) 5' - GCCGCC - 3' 3' - CGGCGG - 5' 
D) 5' - CTGCAG - 3' 3' - GACGTC - 5'

Question 12

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Accepted Answer

A) sequencing ladders 
B) automated sequencing 
C) base-specific DNA cleavage 
D) genomic libraries 
A) sequencing ladders 
B) automated sequencing 
C) base-specific DNA cleavage 
D) genomic libraries

Question 13

Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.

Accepted Answer

A) True 
 B)False

Question 14

Real-time PCR can be used for which of the following?

Accepted Answer

A) Conduct a PCR reaction without the use of primers 
B) Generate cDNA 
C) Quantify expression of a specific gene in a cell 
D) Nonspecific DNA amplification 
A) Conduct a PCR reaction without the use of primers 
B) Generate cDNA 
C) Quantify expression of a specific gene in a cell 
D) Nonspecific DNA amplification

Question 15

What is one technique that is used to identify a specific RNA sequence of a sample from a library?

Accepted Answer

A) Western blotting 
B) Northern blotting 
C) Southern blotting 
D) Eastern blotting 
A) Western blotting 
B) Northern blotting 
C) Southern blotting 
D) Eastern blotting

Question 16

A scientist does not have an antibody to detect the protein of interest, but wants to know if the gene is expressed at low levels. What technique could the researcher use to detect, but not quantify the expression of the gene of interest?

Accepted Answer

A) Western Blotting 
B) real-time PCR 
C) cloning 
D) reverse transcriptase PCR 
A) Western Blotting 
B) real-time PCR 
C) cloning 
D) reverse transcriptase PCR

Question 17

How might a probe for a colony hybridization experiment be obtained?

Accepted Answer

A) The sequence of the gene does not have to be known. 
B) Using the known sequence of a similar gene from a closely related species. 
C) The genomic DNA library. 
D) Using competent cells. 
A) The sequence of the gene does not have to be known. 
B) Using the known sequence of a similar gene from a closely related species. 
C) The genomic DNA library. 
D) Using competent cells.

Question 18

What contains both vector DNA and chromosomal DNA?

Accepted Answer

A) recircularized vectors 
B) recombinant vectors 
C) open vectors 
A) recircularized vectors 
B) recombinant vectors 
C) open vectors

Question 19

What is the origin of restriction endonucleases?

Accepted Answer

A) They are part of DNA repair mechanisms in eukaryotic cells 
B) They are a defense mechanism against viruses in bacteria 
C) They are replication enzymes of yeast 
D) They are transposable elements of Drosophila 
A) They are part of DNA repair mechanisms in eukaryotic cells 
B) They are a defense mechanism against viruses in bacteria 
C) They are replication enzymes of yeast 
D) They are transposable elements of Drosophila

Question 20

A cDNA library differs from a genomic DNA library in which way?

Accepted Answer

A) It consists solely of RNA molecules 
B) It contains many copies of the gene of interest 
C) It contains only coding sequences, not introns 
D) It is typically 10-100 times the size of a DNA library 
A) It consists solely of RNA molecules 
B) It contains many copies of the gene of interest 
C) It contains only coding sequences, not introns 
D) It is typically 10-100 times the size of a DNA library

Exam 20: DNA Technologies

The presence of a specific plasmid in a bacterial cell may be determined if the plasmid _____.

A dideoxy nucleotide is missing an -OH group at the 2' end.

The isolation and copying of a gene, usually in large quantities is called gene cloning.

cDNA is made from mRNA therefore, it lacks introns and contains only the coding sequence for the functional protein.

Dideoxy nucleotides are used in which technique?

Cloned genes may be mutated outside of a cell by a process called in vitro site-directed mutagenesis.

What function does a competent cell have that makes it useful in cloning experiments?

R factors typically contain genes for antibiotic resistance.

What is one technique that is used to identify a specific DNA sequence of a sample from a library?

___________ conditions are essential in a Southern blot experiment if the researcher wants to identify the closest DNA match to the probe.

Which of the following is an example of a palindromic DNA sequence?

The use of fluorescent-labeled dideoxynucleotides is a characteristic of what type of sequencing?

Molecular biologists use restriction enzymes to amplify a specific section of DNA within the genome.

Real-time PCR can be used for which of the following?

What is one technique that is used to identify a specific RNA sequence of a sample from a library?

A scientist does not have an antibody to detect the protein of interest, but wants to know if the gene is expressed at low levels. What technique could the researcher use to detect, but not quantify the expression of the gene of interest?

How might a probe for a colony hybridization experiment be obtained?

What contains both vector DNA and chromosomal DNA?

What is the origin of restriction endonucleases?

A cDNA library differs from a genomic DNA library in which way?

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and Bacteriophages

Variation in Chromosome Structure and Number

Molecular Structure of DNA and RNA

Chromosome Organization and Molecular Structure

DNA Replication

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional Regulation

Gene Regulation in Eukaryotes II: Epigenetics and Regulation at the RNA Level

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular Level

Biotechnology

Genomics I: Analysis of DNA

Genomics II: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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