Exam 16: Forward Genetics and Recombinant DNA Technology

Which type of DNA library (genomic or cDNA)would include introns and promoters?

-Because of redundancy in the genetic code,some amino acids can have more than one possible codon.Use the table above and select which of the answer choices is a possibility for the following amino acid sequence: valine,glycine,glycine,cysteine,tyrosine

A hypothetical restriction sequence is 5′-TCTAGA-3′.The palindromic sequence is 3′-________-5′.

Identifying an autosomal recessive mutation in a mutagenic screen in Drosophila requires identification of a mutant in the F₃ generation.If testing for a sex-linked recessive lethal mutation (e.g.,cn l + using a balancer chromosome such as cnCyO),in which generation can lines with mutations be identified?

One use of PCR has been to monitor the trafficking of endangered organisms.Suppose that one suspect was accused of illegally importing a specific animal,but the suspect asserts that the young animal had been bred in this country from two resident zoo animals.How would you best proceed?

In a given situation,reduced or missing function in gene A results in a viable but noticeable phenotype.The same is true for gene B.However,when both gene A and gene B products are reduced or missing in the same organism,the result is lethality.What is this called?

To produce a molecular clone requires amplification of a sequence via PCR or what other process?

A balancer chromosome includes a recessive lethal allele,a marker dominant allele,and ________.

What method can you use to identify a gene when you know the amino acid sequence of a homologous protein?

In a cDNA library,not all expressed genes will be represented.On what does this depend?