Question 1

Antisense control of translation involves the regulation of which enzyme produced by bacterial insertion sequence IS10?

Accepted Answer

The answer of Antisense control of translation involves the regulation...

Question 2

Mutations of trpL decrease the efficiency of transcriptional regulation by disrupting the formation of which stem-loop structure?

Accepted Answer

3-4 (termi

Question 3

What are the two active sites or "domains" on a repressor protein?

Accepted Answer

DNA-bindin

Question 4

Which sigma factor,encoded by the rpoH gene,is active at high temperatures?

Accepted Answer

A) σ⁷⁰ 
B) σ³² 
C) σ⁴⁵ 
D) σ³⁷ 
E) σ⁵⁰ 
A) σ⁷⁰ 
B) σ³² 
C) σ⁴⁵ 
D) σ³⁷ 
E) σ⁵⁰

Question 5

A bacterium is unable to transport lactose into the cell to be broken down.Which gene is likely mutated in this bacterium?

Accepted Answer

A) lacZ 
B) lacY 
C) lacI 
D) lacP 
E) lacO 
A) lacZ 
B) lacY 
C) lacI 
D) lacP 
E) lacO

Question 6

During the attenuation of the trp operon,which stem loop leads to polycistronic mRNA synthesis during tryptophan starvation?

Accepted Answer

A) 1-3 (antitermination)stem loop 
B) 3-4 (termination)stem loop 
C) 1-2 (pause)stem loop 
D) 2-3 (antitermination)stem loop 
E) 2-4 (termination)stem loop 
A) 1-3 (antitermination)stem loop 
B) 3-4 (termination)stem loop 
C) 1-2 (pause)stem loop 
D) 2-3 (antitermination)stem loop 
E) 2-4 (termination)stem loop

Question 7

Bacterial translation is inhibited by ________ RNA,which is complementary to a portion of a specific mRNA.

Accepted Answer

The answer of Bacterial translation is inhibited by ________ RNA,which...

Question 8

In negative control,what molecule would you expect to find bound to the operator if there is no transcription?

Accepted Answer

A) activator 
B) repressor 
C) inducer 
D) corepressor 
E) RNA polymerase 
A) activator 
B) repressor 
C) inducer 
D) corepressor 
E) RNA polymerase

Question 9

Regulation of transcription of bacterial genes takes place at which two levels?

Accepted Answer

initiation

Question 10

You want to design a repressor protein mutant.Which protein domain is the best target for preventing binding of the corepressor?

Accepted Answer

A) DNA-binding domain 
B) allosteric domain 
C) promoter domain 
D) helix-turn-helix domain 
E) activator binding site 
A) DNA-binding domain 
B) allosteric domain 
C) promoter domain 
D) helix-turn-helix domain 
E) activator binding site

Question 11

In λ phage,entry into the ________ cycle requires transcription of late genes.

Accepted Answer

The answer of In λ phage,entry into the ________ cycle...

Question 12

Which part of the lac operon produces a regulatory protein that is trans-acting?

Accepted Answer

The answer of Which part of the lac operon produces...

Question 13

What are the three possible alternative stem loops that can form in mRNA?

Accepted Answer

pause,anti

Question 14

In the absence of tryptophan,

Accepted Answer

A) the inactive repressor cannot bind trpO,so operon gene transcription occurs. 
B) the active repressor binds trpP,so operon gene transcription is repressed. 
C) the inducer cannot bind trpO,so operon gene transcription occurs. 
D) the active repressor cannot bind trpO,so operon gene transcription is attenuated. 
E) the repressor binds the corepressor,and operon gene transcription occurs. 
A) the inactive repressor cannot bind trpO,so operon gene transcription occurs. 
B) the active repressor binds trpP,so operon gene transcription is repressed. 
C) the inducer cannot bind trpO,so operon gene transcription occurs. 
D) the active repressor cannot bind trpO,so operon gene transcription is attenuated. 
E) the repressor binds the corepressor,and operon gene transcription occurs.

Question 15

Which of the following are constitutive mutants of the lac operon?

Accepted Answer

A) lacZ and lacP 
B) lacZ and lacI 
C) lacO and lacI 
D) lacO and lacZ 
E) lacI and lacY 
A) lacZ and lacP 
B) lacZ and lacI 
C) lacO and lacI 
D) lacO and lacZ 
E) lacI and lacY

Question 16

In λ phage,which protein,the product of the cI gene,blocks the transcription required to initiate the lytic cycle?

Accepted Answer

A) cro 
B) repressor 
C) enhancer 
D) integrase 
E) operator 
A) cro 
B) repressor 
C) enhancer 
D) integrase 
E) operator

Question 17

Which region of the lambda phage genome enables the linear chromosome to circularize when it enters a host cell?

Accepted Answer

A) cohesive (cos)site 
B) IS10 
C) early operators 
D) integrase (int)gene 
E) excisionase (xis)gene 
A) cohesive (cos)site 
B) IS10 
C) early operators 
D) integrase (int)gene 
E) excisionase (xis)gene

Question 18

Which enzyme is the product of the lacZ gene?

Accepted Answer

The answer of Which enzyme is the product of the...

Question 19

Describe the difference between inducible and repressible operons.How does attenuation differ from inducible or repressible operons?
Which of the two inducible operons you've studied would be active if a cell has the following food sources?
a. only lactose
b. only glucose
c. only arabinose
d. lactose and glucose
e. glucose and arabinose
f. lactose and arabinose

Accepted Answer

Operons like lac and ara that are involv

Question 20

In the trp operon,where is the attenuator region located?

Accepted Answer

The answer of In the trp operon,where is the attenuator...

Exam 14: Regulation of Gene Expression in Bacteria and Bacteriophage

Antisense control of translation involves the regulation of which enzyme produced by bacterial insertion sequence IS10?

Mutations of trpL decrease the efficiency of transcriptional regulation by disrupting the formation of which stem-loop structure?

What are the two active sites or "domains" on a repressor protein?

Which sigma factor,encoded by the rpoH gene,is active at high temperatures?

A bacterium is unable to transport lactose into the cell to be broken down.Which gene is likely mutated in this bacterium?

During the attenuation of the trp operon,which stem loop leads to polycistronic mRNA synthesis during tryptophan starvation?

Bacterial translation is inhibited by ________ RNA,which is complementary to a portion of a specific mRNA.

In negative control,what molecule would you expect to find bound to the operator if there is no transcription?

Regulation of transcription of bacterial genes takes place at which two levels?

You want to design a repressor protein mutant.Which protein domain is the best target for preventing binding of the corepressor?

In λ phage,entry into the ________ cycle requires transcription of late genes.

Which part of the lac operon produces a regulatory protein that is trans-acting?

What are the three possible alternative stem loops that can form in mRNA?

In the absence of tryptophan,

Which of the following are constitutive mutants of the lac operon?

In λ phage,which protein,the product of the cI gene,blocks the transcription required to initiate the lytic cycle?

Which region of the lambda phage genome enables the linear chromosome to circularize when it enters a host cell?

Which enzyme is the product of the lacZ gene?

In the trp operon,where is the attenuator region located?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

DNA Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, DNA Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant DNA Technology

Applications of Recombinant DNA Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

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