Exam 18: Methods in Microbial Ecology

Robotic systems used to quickly test thousands of combinations of nutrients for enrichment of a particular prokaryotic species is an example of a(n)________ technology.

The rolled tube method is most commonly used for isolating which grouping of microorganisms?

Several fluorogenic dyes can be used simultaneously in a sample as long as they each emit light at a different measurable wavelength.

Microarrays are useful for assessing

Winogradsky columns are used primarily for enrichment of

Interpreting stable isotopic fractionation depends on the fact that most enzymes preferentially bind ________ isotope substrates over ________ isotope substrates.

In natural samples it is often difficult to differentiate live cells from dead cells or cells from nonliving matter.

The ¹³C/¹²C isotopic ratio of geological rocks of different ages has been used as evidence for or against past biological activity,because compounds of biological origin

Strict anaerobic organisms are not suited for GFP reporters due to the protein requiring oxygen to fluoresce.

Enrichment cultures can be established by placing the inoculum into selective media and incubating under specific conditions.

Amplification of chromosomal DNA from a single-cell is accomplished by

How can NanoSIMS be used to determine which Roseobacters most strongly use DMSP as a sulfur source within a coastal marine environment? Explain the NanoSIMS methodology in your answer.

Transcription of various genes under particular environmental conditions can be studied by

Which technique would be used to estimate the concentration of naturally occurring Escherichia coli in a wastewater sample?

Which of the following techniques can link prokaryotic phylogeny and function?

Culture-independent approaches avoid issues associated with

Radioisotopes are useful when

Which fluorescent molecule enables visualization of living microorganisms?

Analyses of sulfur isotopes have been used as evidence against life on the Moon,because the sulfides in lunar rocks have

Well-designed microbial activity measurements can reveal ________ of major metabolic reactions in a habitat.