Question 1

The MOST effective antibiotics in preventing growth of disease-causing bacteria are

Accepted Answer

A)  bacteriocidal. 
B)  bacteriostatic. 
C)  bacteriolytic. 
D)  bacteriolytic or bacteriocidal. 
A)  bacteriocidal. 
B)  bacteriostatic. 
C)  bacteriolytic. 
D)  bacteriolytic or bacteriocidal.

Question 2

An unpressurized autoclave would not be able to sterilize a growth medium with contaminating bacteria but could still pasteurize it.

Accepted Answer

A) True 
 B)False

Question 3

What temperature is most commonly used for autoclaves to sterilize growth media and other devices prior to experimentation?

Accepted Answer

A)  95°C 
B)  101°C 
C)  121°C 
D)  140°C 
A)  95°C 
B)  101°C 
C)  121°C 
D)  140°C

Question 4

The addition of thioglycylate into a growth medium would NOT support the growth of a(n)

Accepted Answer

A)  aerotolerant anaerobe. 
B)  facultative aerobe. 
C)  microaerophilic aerobe. 
D)  obligate anaerobe. 
A)  aerotolerant anaerobe. 
B)  facultative aerobe. 
C)  microaerophilic aerobe. 
D)  obligate anaerobe.

Question 5

When viable cell concentrations are too high to count on an agar medium,it is most common to use larger sized plates to increase the surface area for counting the colonies.

Accepted Answer

A) True 
 B)False

Question 6

A bacterium such as a snow alga that is able to survive a cold temperature is called a psychrophile.

Accepted Answer

A) True 
 B)False

Question 7

The activity of MinC and MinD direct whether a bacterial cell will be coccoid or bacillus shaped.

Accepted Answer

A) True 
 B)False

Question 8

Superoxide dismutase and catalase work together to convert superoxide into

Accepted Answer

A)  peroxide. 
B)  oxygen. 
C)  ozone. 
D)  water. 
A)  peroxide. 
B)  oxygen. 
C)  ozone. 
D)  water.

Question 9

During elongation of a cell during binary fission,small gaps caused by transglycosylases are formed before cell membrane precursors can be inserted.

Accepted Answer

A) True 
 B)False

Question 10

Some microbes can grow in boiling water.

Accepted Answer

A) True 
 B)False

Question 11

The rates of exponential growth (in the exponential phase)vary greatly according to the bacterial species as well as the bioavailable nutrients.

Accepted Answer

A) True 
 B)False

Question 12

For most purposes of studying bacterial isolates,viable counting usually gives accurate information about the number of active cells present in a culture volume.

Accepted Answer

A) True 
 B)False

Question 13

An antibiotic that increased the activity of ________ would likely result in cell death.

Accepted Answer

A)  autolysin 
B)  binary fission 
C)  stationary growth 
D)  ionic bonds in proteins 
A)  autolysin 
B)  binary fission 
C)  stationary growth 
D)  ionic bonds in proteins

Question 14

Most mesophilic organisms can grow in a temperature range of

Accepted Answer

A)  0-15°C. 
B)  10-20°C. 
C)  20-40°C. 
D)  50-65°C. 
A)  0-15°C. 
B)  10-20°C. 
C)  20-40°C. 
D)  50-65°C.

Question 15

The optimum temperature is usually closer to the minimum temperature than the maximum temperature.

Accepted Answer

A) True 
 B)False

Question 16

The sensitivity of DNA and RNA is one major reason water activity can limit microbial growth.

Accepted Answer

A) True 
 B)False

Question 17

Identifying bactoprenol inside the rare bacteria that lack peptidoglycan would be unexpected considering its role in membrane biosynthesis.

Accepted Answer

A) True 
 B)False

Question 18

Consider solution A (pH 6)and solution B (pH 9).Which of the following statements is CORRECT?

Accepted Answer

A)  Solution A is 3 times more acidic than solution B. 
B)  Solution A is 300 times more acidic than solution B. 
C)  Solution B is 1,000 times more alkaline (basic) than solution A. 
D)  Solution B is 3,000 times more alkaline (basic) than solution A. 
A)  Solution A is 3 times more acidic than solution B. 
B)  Solution A is 300 times more acidic than solution B. 
C)  Solution B is 1,000 times more alkaline (basic) than solution A. 
D)  Solution B is 3,000 times more alkaline (basic) than solution A.

Question 19

Compare and contrast the spread plate method and the pour plate method of doing plate counts.Also describe which group of organisms would not be quantifiable in the pour plate method but would still be observed in the spread plate method.

Accepted Answer

Answers will vary,but one benefit to the

Question 20

A non-aerated or shaken liquid growth medium inoculated with a bacterium is one of the best ways to culture an obligate anaerobe.

Accepted Answer

A) True 
 B)False

Exam 5: Microbial Growth and Control

The MOST effective antibiotics in preventing growth of disease-causing bacteria are

An unpressurized autoclave would not be able to sterilize a growth medium with contaminating bacteria but could still pasteurize it.

What temperature is most commonly used for autoclaves to sterilize growth media and other devices prior to experimentation?

The addition of thioglycylate into a growth medium would NOT support the growth of a(n)

When viable cell concentrations are too high to count on an agar medium,it is most common to use larger sized plates to increase the surface area for counting the colonies.

A bacterium such as a snow alga that is able to survive a cold temperature is called a psychrophile.

The activity of MinC and MinD direct whether a bacterial cell will be coccoid or bacillus shaped.

Superoxide dismutase and catalase work together to convert superoxide into

During elongation of a cell during binary fission,small gaps caused by transglycosylases are formed before cell membrane precursors can be inserted.

Some microbes can grow in boiling water.

The rates of exponential growth (in the exponential phase)vary greatly according to the bacterial species as well as the bioavailable nutrients.

For most purposes of studying bacterial isolates,viable counting usually gives accurate information about the number of active cells present in a culture volume.

An antibiotic that increased the activity of ________ would likely result in cell death.

Most mesophilic organisms can grow in a temperature range of

The optimum temperature is usually closer to the minimum temperature than the maximum temperature.

The sensitivity of DNA and RNA is one major reason water activity can limit microbial growth.

Identifying bactoprenol inside the rare bacteria that lack peptidoglycan would be unexpected considering its role in membrane biosynthesis.

Consider solution A (pH 6)and solution B (pH 9).Which of the following statements is CORRECT?

Compare and contrast the spread plate method and the pour plate method of doing plate counts.Also describe which group of organisms would not be quantifiable in the pour plate method but would still be observed in the spread plate method.

A non-aerated or shaken liquid growth medium inoculated with a bacterium is one of the best ways to culture an obligate anaerobe.

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