Question 1

It is often not possible to determine the exact sequence of large polynucleotides such as RNA and DNA.

Accepted Answer

A) True 
 B)False

Question 2

Purines which are found mainly in both deoxyribonucleotides and ribonucleotides are

Accepted Answer

A) thymine and cytosine. 
B) thymine,cytosine and uracil. 
C) adenine and thymine. 
D) guanine and cytosine. 
E) adenine and guanine. 
A) thymine and cytosine. 
B) thymine,cytosine and uracil. 
C) adenine and thymine. 
D) guanine and cytosine. 
E) adenine and guanine.

Question 3

Which is not a biological function of RNA?

Accepted Answer

A) Participates in the modification of newly synthesized RNA. 
B) It is the genome of some viruses. 
C) Serves as a scaffold component for chromosomes. 
D) Composes an integral part of the ribosome. 
E) None of the above;all are functions of RNA. 
A) Participates in the modification of newly synthesized RNA. 
B) It is the genome of some viruses. 
C) Serves as a scaffold component for chromosomes. 
D) Composes an integral part of the ribosome. 
E) None of the above;all are functions of RNA.

Question 4

Much of the stability of the double-stranded DNA structure is the result of

Accepted Answer

A) hydrogen bonding between purines. 
B) the phosphodiester backbone. 
C) the angle of the planes of the bases with respect to the helix axis. 
D) the stacking interactions between base pair. 
A) hydrogen bonding between purines. 
B) the phosphodiester backbone. 
C) the angle of the planes of the bases with respect to the helix axis. 
D) the stacking interactions between base pair.

Question 5

The tetranucleotide AGTC (in DNA)has a free hydroxyl group on

Accepted Answer

A) A. 
B) A,G,T,and C. 
C) C. 
D) G,T,and C. 
A) A. 
B) A,G,T,and C. 
C) C. 
D) G,T,and C.

Question 6

Deoxythymidine is a ________ found mainly in DNA.

Accepted Answer

A) nucleotide 
B) deoxynucleotide 
C) nucleoside 
D) deoxynucleoside 
E) glycoside 
A) nucleotide 
B) deoxynucleotide 
C) nucleoside 
D) deoxynucleoside 
E) glycoside

Question 7

Examine the data given for a set of endonuclease digests of a 1000 bp DNA fragment.Then choose the restriction sites for Enzyme #2 on the restriction map shown.The restriction map displays all sites for both enzymes. Digestion with Enzyme #1 produces fragments of 200 bp and 400 bp.
Digestion with Enzyme #2 produces fragments of 200 bp,300 bp and 500 bp.
Simultaneous digestion with both enzymes yields fragments of 100 bp,200 bp and 300bp

Accepted Answer

A) Sites W and Y 
B) Sites X,Y and Z 
C) Sites X and Z 
D) Sites W and X 
A) Sites W and Y 
B) Sites X,Y and Z 
C) Sites X and Z 
D) Sites W and X

Question 8

Which are the products of the RNase A-catalyzed hydrolysis of pGpApUpApApCpG?

Accepted Answer

A) pG + pA + pUpA + pA + pCpG 
B) pGpApU + pApApC + pG 
C) pG + pApUpApApCpG 
D) pGpApUp + ApApCp + G 
E) two pG + three pA + pU + pC 
A) pG + pA + pUpA + pA + pCpG 
B) pGpApU + pApApC + pG 
C) pG + pApUpApApCpG 
D) pGpApUp + ApApCp + G 
E) two pG + three pA + pU + pC

Question 9

What is the effect of incubating a mixture of RNA and DNA with 0.1 M NaOH?

Accepted Answer

A) RNA is hydrolyzed to nucleoside monophosphates. 
B) DNA is hydrolyzed to nucleoside monophosphates. 
C) Both RNA and DNA are hydrolyzed to nucleoside monophosphates. 
D) No effect.RNA and DNA are resistant to base hydrolysis. 
A) RNA is hydrolyzed to nucleoside monophosphates. 
B) DNA is hydrolyzed to nucleoside monophosphates. 
C) Both RNA and DNA are hydrolyzed to nucleoside monophosphates. 
D) No effect.RNA and DNA are resistant to base hydrolysis.

Question 10

What is the approximate diameter of a chromatin fiber?

Accepted Answer

A) 3 nm 
B) 30 nm 
C) 300 nm 
D) 3 mm 
E) 30 mm 
A) 3 nm 
B) 30 nm 
C) 300 nm 
D) 3 mm 
E) 30 mm

Question 11

Protein X recognizes and binds to a particular (normal and undamaged)DNA sequence.Which statement applies?

Accepted Answer

A) The protein can recognize the sequence by accessing the bases at the major or minor groove. 
B) The protein can recognize sequence-dependent differences in the distance between the sugar-phosphate backbones of the two strands. 
C) The protein must have a cofactor to separate the DNA strands to access and recognize the sequence. 
D) The protein must be dimeric to recognize the complementary sequences on each strand. 
A) The protein can recognize the sequence by accessing the bases at the major or minor groove. 
B) The protein can recognize sequence-dependent differences in the distance between the sugar-phosphate backbones of the two strands. 
C) The protein must have a cofactor to separate the DNA strands to access and recognize the sequence. 
D) The protein must be dimeric to recognize the complementary sequences on each strand.

Question 12

In DNA,phosphodiester bonds form with hydroxyl groups of carbons number ________ and ________ of each deoxyribose sugar.

Accepted Answer

A) 2';3' 
B) 2';4' 
C) 3';4' 
D) 3';5' 
E) 4';5' 
A) 2';3' 
B) 2';4' 
C) 3';4' 
D) 3';5' 
E) 4';5'

Question 13

What is the order of packaging of chromatin in eukaryotic cells from smallest to largest size?

Accepted Answer

A) double-helix → solenoid → nucleosome → chromosome 
B) double-helix → chromatin → solenoid → chromosome 
C) single-stranded DNA → chromatin → histones → chromosome 
D) double-helix → nucleosomes → solenoid → chromosome 
A) double-helix → solenoid → nucleosome → chromosome 
B) double-helix → chromatin → solenoid → chromosome 
C) single-stranded DNA → chromatin → histones → chromosome 
D) double-helix → nucleosomes → solenoid → chromosome

Question 14

Which of the following is mismatched?

Accepted Answer

A) B-DNA: dehydrated. 
B) Z-DNA: left-handed helix. 
C) A-DNA: dehydrated. 
D) Z-DNA: no major or minor grooves. 
E) B-DNA: right-handed helix. 
A) B-DNA: dehydrated. 
B) Z-DNA: left-handed helix. 
C) A-DNA: dehydrated. 
D) Z-DNA: no major or minor grooves. 
E) B-DNA: right-handed helix.

Question 15

________ plays an important role in storing the energy needed for the unwinding of DNA during replication,recombination,repair and transcription.

Accepted Answer

A) A high percent of G:C base pairs 
B) The charge on basic side chains of histone proteins 
C) Negative supercoiling 
D) Overwinding of the helix 
A) A high percent of G:C base pairs 
B) The charge on basic side chains of histone proteins 
C) Negative supercoiling 
D) Overwinding of the helix

Question 16

RNA does not have a double strand form.

Accepted Answer

A) True 
 B)False

Question 17

Prokaryotic DNA is organized into protein-DNA structures called nucleoids.

Accepted Answer

A) True 
 B)False

Question 18

Adenylate and AMP each contain ________ phosphate group(s).

Accepted Answer

A) 0 
B) 1 
C) 2 
D) 3 
E) None of the above 
A) 0 
B) 1 
C) 2 
D) 3 
E) None of the above

Question 19

Messenger RNA is the least stable type of cellular ribonucleic acid.

Accepted Answer

A) True 
 B)False

Question 20

Strands in the stem of stem-loop RNA structures are parallel.

Accepted Answer

A) True 
 B)False

Exam 19: Nucleic Acids

It is often not possible to determine the exact sequence of large polynucleotides such as RNA and DNA.

Purines which are found mainly in both deoxyribonucleotides and ribonucleotides are

Which is not a biological function of RNA?

Much of the stability of the double-stranded DNA structure is the result of

The tetranucleotide AGTC (in DNA)has a free hydroxyl group on

Deoxythymidine is a ________ found mainly in DNA.

Which are the products of the RNase A-catalyzed hydrolysis of pGpApUpApApCpG?

What is the effect of incubating a mixture of RNA and DNA with 0.1 M NaOH?

What is the approximate diameter of a chromatin fiber?

Protein X recognizes and binds to a particular (normal and undamaged)DNA sequence.Which statement applies?

In DNA,phosphodiester bonds form with hydroxyl groups of carbons number ________ and ________ of each deoxyribose sugar.

What is the order of packaging of chromatin in eukaryotic cells from smallest to largest size?

Which of the following is mismatched?

________ plays an important role in storing the energy needed for the unwinding of DNA during replication,recombination,repair and transcription.

RNA does not have a double strand form.

Prokaryotic DNA is organized into protein-DNA structures called nucleoids.

Adenylate and AMP each contain ________ phosphate group(s).

Messenger RNA is the least stable type of cellular ribonucleic acid.

Strands in the stem of stem-loop RNA structures are parallel.

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Atp Synthesis

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

DNA Replication, repair, and Recombination

Transcription and RNA Processing

Protein Synthesis

Filters

In DNA,phosphodiester bonds form with hydroxyl groups of carbons number and of each deoxyribose sugar.